First description and evaluation of SNPs in the ADH and ALDH genes in a population of alcoholics in Central-West Brazil
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.alcohol.2017.04.006 http://hdl.handle.net/11449/163505 |
Resumo: | Worldwide, different studies have reported an association of alcohol-use disorder (AUD) with different types of Single Nucleotide Polymorphisms (SNPs) in the genes for aldehyde dehydrogenase (ALDH) and alcohol dehydrogenase (ADH). In Brazil, there is little information about the occurrence of these SNPs in the AUD population and an absence of studies characterizing the population in the Central-West Region of Brazil. Actually, in Brazil, there are more than 4 million people with AUD. Despite the major health hazards of AUD, information on alcohol consumption and its consequences are not well understood. Therefore, it is extremely important to characterize these SNPs for the better understanding of AUD as a genetic disease in the Brazilian population. The present study, unlike other studies in other countries, is done with a subject population that shows a significant amount of racial homogenization. We evaluated the presence of SNPs in the ADH (ADH1B, ADH1C, and ADH4) and ALDH (ALDH2) genes in alcohol users of Goiania, State of Goias - Brazil, and then we established a possible relationship with AUD by allelic and genotypic study. This study was conducted with a population of people with AUD (n = 99) from Goias Alcohol Dependence Recovery Center (GO CEREA) and Psychosocial Care Center for Alcohol and Drugs (CAPS AD), and with a population of people without AUD as controls (n = 100). DNA was extracted from whole-blood samples and the genotyping was performed using TaqMan (R) SNP genotyping assays. For characterization and evaluation of SNPs in the population, genotype frequency, allele frequency, haplotype frequency, Hardy-Weinberg equilibrium, and linkage disequilibrium were analyzed. Statistical analyses were calculated by GENEPOP 4.5 and Haploview software. The allele 1 was considered as wild (or *1) and allele 2 as mutant (or *2). Significant differences were found for ADH1B*, ADH4*2, and ALDH2*2 SNPs when the genotype and allele frequencies were analyzed. In addition, four haplotypes were observed between ADH1B*2 and ADH1C*2 through linkage disequilibrium analysis. The genetic variants may be associated with protection against AUD in the population studied. (C) 2017 Elsevier Inc. All rights reserved. |
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First description and evaluation of SNPs in the ADH and ALDH genes in a population of alcoholics in Central-West BrazilADHAlcohol-use disorderALDHLinkage disequilibriumPolymorphismWorldwide, different studies have reported an association of alcohol-use disorder (AUD) with different types of Single Nucleotide Polymorphisms (SNPs) in the genes for aldehyde dehydrogenase (ALDH) and alcohol dehydrogenase (ADH). In Brazil, there is little information about the occurrence of these SNPs in the AUD population and an absence of studies characterizing the population in the Central-West Region of Brazil. Actually, in Brazil, there are more than 4 million people with AUD. Despite the major health hazards of AUD, information on alcohol consumption and its consequences are not well understood. Therefore, it is extremely important to characterize these SNPs for the better understanding of AUD as a genetic disease in the Brazilian population. The present study, unlike other studies in other countries, is done with a subject population that shows a significant amount of racial homogenization. We evaluated the presence of SNPs in the ADH (ADH1B, ADH1C, and ADH4) and ALDH (ALDH2) genes in alcohol users of Goiania, State of Goias - Brazil, and then we established a possible relationship with AUD by allelic and genotypic study. This study was conducted with a population of people with AUD (n = 99) from Goias Alcohol Dependence Recovery Center (GO CEREA) and Psychosocial Care Center for Alcohol and Drugs (CAPS AD), and with a population of people without AUD as controls (n = 100). DNA was extracted from whole-blood samples and the genotyping was performed using TaqMan (R) SNP genotyping assays. For characterization and evaluation of SNPs in the population, genotype frequency, allele frequency, haplotype frequency, Hardy-Weinberg equilibrium, and linkage disequilibrium were analyzed. Statistical analyses were calculated by GENEPOP 4.5 and Haploview software. The allele 1 was considered as wild (or *1) and allele 2 as mutant (or *2). Significant differences were found for ADH1B*, ADH4*2, and ALDH2*2 SNPs when the genotype and allele frequencies were analyzed. In addition, four haplotypes were observed between ADH1B*2 and ADH1C*2 through linkage disequilibrium analysis. The genetic variants may be associated with protection against AUD in the population studied. (C) 2017 Elsevier Inc. All rights reserved.FAPEGCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Goias, Inst Ciencias Biol, Lab Genet Mol & Citogenet, Ave Esperanca S-N,Campus Samambaia Campus 2, BR-74690900 Goiania, Go, BrazilUniv Estadual Paulista, Inst Biociencias Botucatu, Lab Pesquisa & Anal Genet, Sao Paulo, BrazilUniv Fed Goias, Inst Ciencias Biol, Lab Mutagenese, Goiania, Go, BrazilUniv Fed Goias, Inst Ciencias Biol, Lab Genet & Biodiversidade, Goiania, Go, BrazilUniv Estadual Paulista, Inst Biociencias Botucatu, Lab Pesquisa & Anal Genet, Sao Paulo, BrazilFAPEG: 006/2012FAPEG: 2012102670001220Elsevier B.V.Universidade Federal de Goiás (UFG)Universidade Estadual Paulista (Unesp)Teixeira, Thallita MonteiroSilva, Hugo Delleon daGoveia, Rebeca MotaMartins Ribolla, Paulo Eduardo [UNESP]Alonso, Diego Peres [UNESP]Alves, Alessandro ArrudaMelo e Silva, DanielaCollevatti, Rosane GarciaBicudo, Lucilene ArilhoBergamo, Nadia AparecidaSilveira-Lacerda, Elisangela de Paula2018-11-26T17:42:18Z2018-11-26T17:42:18Z2017-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article37-43application/pdfhttp://dx.doi.org/10.1016/j.alcohol.2017.04.006Alcohol. New York: Elsevier Science Inc, v. 65, p. 37-43, 2017.0741-8329http://hdl.handle.net/11449/16350510.1016/j.alcohol.2017.04.006WOS:000415910000006WOS000415910000006.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAlcohol1,153info:eu-repo/semantics/openAccess2024-01-29T06:29:44Zoai:repositorio.unesp.br:11449/163505Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:13:50.879182Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
First description and evaluation of SNPs in the ADH and ALDH genes in a population of alcoholics in Central-West Brazil |
title |
First description and evaluation of SNPs in the ADH and ALDH genes in a population of alcoholics in Central-West Brazil |
spellingShingle |
First description and evaluation of SNPs in the ADH and ALDH genes in a population of alcoholics in Central-West Brazil Teixeira, Thallita Monteiro ADH Alcohol-use disorder ALDH Linkage disequilibrium Polymorphism |
title_short |
First description and evaluation of SNPs in the ADH and ALDH genes in a population of alcoholics in Central-West Brazil |
title_full |
First description and evaluation of SNPs in the ADH and ALDH genes in a population of alcoholics in Central-West Brazil |
title_fullStr |
First description and evaluation of SNPs in the ADH and ALDH genes in a population of alcoholics in Central-West Brazil |
title_full_unstemmed |
First description and evaluation of SNPs in the ADH and ALDH genes in a population of alcoholics in Central-West Brazil |
title_sort |
First description and evaluation of SNPs in the ADH and ALDH genes in a population of alcoholics in Central-West Brazil |
author |
Teixeira, Thallita Monteiro |
author_facet |
Teixeira, Thallita Monteiro Silva, Hugo Delleon da Goveia, Rebeca Mota Martins Ribolla, Paulo Eduardo [UNESP] Alonso, Diego Peres [UNESP] Alves, Alessandro Arruda Melo e Silva, Daniela Collevatti, Rosane Garcia Bicudo, Lucilene Arilho Bergamo, Nadia Aparecida Silveira-Lacerda, Elisangela de Paula |
author_role |
author |
author2 |
Silva, Hugo Delleon da Goveia, Rebeca Mota Martins Ribolla, Paulo Eduardo [UNESP] Alonso, Diego Peres [UNESP] Alves, Alessandro Arruda Melo e Silva, Daniela Collevatti, Rosane Garcia Bicudo, Lucilene Arilho Bergamo, Nadia Aparecida Silveira-Lacerda, Elisangela de Paula |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Goiás (UFG) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Teixeira, Thallita Monteiro Silva, Hugo Delleon da Goveia, Rebeca Mota Martins Ribolla, Paulo Eduardo [UNESP] Alonso, Diego Peres [UNESP] Alves, Alessandro Arruda Melo e Silva, Daniela Collevatti, Rosane Garcia Bicudo, Lucilene Arilho Bergamo, Nadia Aparecida Silveira-Lacerda, Elisangela de Paula |
dc.subject.por.fl_str_mv |
ADH Alcohol-use disorder ALDH Linkage disequilibrium Polymorphism |
topic |
ADH Alcohol-use disorder ALDH Linkage disequilibrium Polymorphism |
description |
Worldwide, different studies have reported an association of alcohol-use disorder (AUD) with different types of Single Nucleotide Polymorphisms (SNPs) in the genes for aldehyde dehydrogenase (ALDH) and alcohol dehydrogenase (ADH). In Brazil, there is little information about the occurrence of these SNPs in the AUD population and an absence of studies characterizing the population in the Central-West Region of Brazil. Actually, in Brazil, there are more than 4 million people with AUD. Despite the major health hazards of AUD, information on alcohol consumption and its consequences are not well understood. Therefore, it is extremely important to characterize these SNPs for the better understanding of AUD as a genetic disease in the Brazilian population. The present study, unlike other studies in other countries, is done with a subject population that shows a significant amount of racial homogenization. We evaluated the presence of SNPs in the ADH (ADH1B, ADH1C, and ADH4) and ALDH (ALDH2) genes in alcohol users of Goiania, State of Goias - Brazil, and then we established a possible relationship with AUD by allelic and genotypic study. This study was conducted with a population of people with AUD (n = 99) from Goias Alcohol Dependence Recovery Center (GO CEREA) and Psychosocial Care Center for Alcohol and Drugs (CAPS AD), and with a population of people without AUD as controls (n = 100). DNA was extracted from whole-blood samples and the genotyping was performed using TaqMan (R) SNP genotyping assays. For characterization and evaluation of SNPs in the population, genotype frequency, allele frequency, haplotype frequency, Hardy-Weinberg equilibrium, and linkage disequilibrium were analyzed. Statistical analyses were calculated by GENEPOP 4.5 and Haploview software. The allele 1 was considered as wild (or *1) and allele 2 as mutant (or *2). Significant differences were found for ADH1B*, ADH4*2, and ALDH2*2 SNPs when the genotype and allele frequencies were analyzed. In addition, four haplotypes were observed between ADH1B*2 and ADH1C*2 through linkage disequilibrium analysis. The genetic variants may be associated with protection against AUD in the population studied. (C) 2017 Elsevier Inc. All rights reserved. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-01 2018-11-26T17:42:18Z 2018-11-26T17:42:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.alcohol.2017.04.006 Alcohol. New York: Elsevier Science Inc, v. 65, p. 37-43, 2017. 0741-8329 http://hdl.handle.net/11449/163505 10.1016/j.alcohol.2017.04.006 WOS:000415910000006 WOS000415910000006.pdf |
url |
http://dx.doi.org/10.1016/j.alcohol.2017.04.006 http://hdl.handle.net/11449/163505 |
identifier_str_mv |
Alcohol. New York: Elsevier Science Inc, v. 65, p. 37-43, 2017. 0741-8329 10.1016/j.alcohol.2017.04.006 WOS:000415910000006 WOS000415910000006.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Alcohol 1,153 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
37-43 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129598005706752 |