First Chromosomal Analysis in Hepsetidae (Actinopterygii, Characiformes): Insights into Relationship between African and Neotropical Fish Groups
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fgene.2017.00203 http://hdl.handle.net/11449/163618 |
Resumo: | Hepsetidae is a small fish family with only the genus Hepsetus, with six described species distributed throughout the South, Central and Western regions of Africa, showing a close relationship with the Alestidae and some Neotropical fish families. However, no cytogenetic information is available for both Hepsetidae and Alestidae species, thus preventing any evolutionary comparative studies at the chromosomal level. In the present study, we are providing new cytogenetic data for Hepsetus odoe, including the standard karyotype, C-banding, repetitive DNAs mapping, comparative genomic hybridization (CGH) and whole chromosome painting (WCP), providing chromosomal patterns and subsidies for comparative cytogenetics with other characiform families. Both males and females H. odoe have 2n = 58 chromosomes (10m + 28sm + 20st/a), with most of the C-band positive heterochromatin localized in the centromeric and subtelomeric regions. Only one pair of chromosomes bears proximal 5S rDNA sites in the short arms, contrasting with the 18S rDNA sequences which are located in the terminal regions of four chromosome pairs. Clear interstitial hybridization signals are evidenced for the U1 and U2 snDNA probes, but in only one and two chromosome pairs, respectively. Microsatellite motifs are widely distributed in the karyotype, with exception for the (CGG)(10), (GAA)(10) and (GAG)(10) probes, which highlight conspicuous interstitial signals on an unique pair of chromosomes. Comparative data from conventional and molecular cytogenetics, including CGH and WCP experiments, indicate that H. odoe and some Erythrinidae species, particularly Erythrinus erythrinus, share similar chromosomal sequences suggesting some relatedness among them, although bearing genomic specificities in view of their divergent evolutionary histories. |
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First Chromosomal Analysis in Hepsetidae (Actinopterygii, Characiformes): Insights into Relationship between African and Neotropical Fish Groupsfishesmolecular cytogeneticschromosomal paintingcomparative genomic hybridization (CGH)karyotype evolutionHepsetidae is a small fish family with only the genus Hepsetus, with six described species distributed throughout the South, Central and Western regions of Africa, showing a close relationship with the Alestidae and some Neotropical fish families. However, no cytogenetic information is available for both Hepsetidae and Alestidae species, thus preventing any evolutionary comparative studies at the chromosomal level. In the present study, we are providing new cytogenetic data for Hepsetus odoe, including the standard karyotype, C-banding, repetitive DNAs mapping, comparative genomic hybridization (CGH) and whole chromosome painting (WCP), providing chromosomal patterns and subsidies for comparative cytogenetics with other characiform families. Both males and females H. odoe have 2n = 58 chromosomes (10m + 28sm + 20st/a), with most of the C-band positive heterochromatin localized in the centromeric and subtelomeric regions. Only one pair of chromosomes bears proximal 5S rDNA sites in the short arms, contrasting with the 18S rDNA sequences which are located in the terminal regions of four chromosome pairs. Clear interstitial hybridization signals are evidenced for the U1 and U2 snDNA probes, but in only one and two chromosome pairs, respectively. Microsatellite motifs are widely distributed in the karyotype, with exception for the (CGG)(10), (GAA)(10) and (GAG)(10) probes, which highlight conspicuous interstitial signals on an unique pair of chromosomes. Comparative data from conventional and molecular cytogenetics, including CGH and WCP experiments, indicate that H. odoe and some Erythrinidae species, particularly Erythrinus erythrinus, share similar chromosomal sequences suggesting some relatedness among them, although bearing genomic specificities in view of their divergent evolutionary histories.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Fed Sao Carlos, Dept Genet & Evolucao, Sao Carlos, SP, BrazilEstado Educ Mato Grosso Seduc MT, Cuiaba, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Morfol, Botucatu, SP, BrazilRoyal Museum Cent Africa, Sect Vertebrates Ichthyol, Tervuren, BelgiumAdamawa State Univ, Dept Fisheries & Aquaculture, Mubi, NigeriaUniv Hosp Jena, Inst Human Genet, Jena, GermanyUniv Estadual Paulista, Inst Biociencias, Dept Morfol, Botucatu, SP, BrazilCNPq: 304992/2015-1CNPq: 401575/2016-0FAPESP: 2016/21411-7FAPESP: 2016/17556-0FAPESP: 2017/08471-3FAPESP: FAPESP 2014/26508-3FAPESP: CNPq 306054/2006-0Frontiers Media SaUniversidade Federal de São Carlos (UFSCar)Estado Educ Mato Grosso Seduc MTUniversidade Estadual Paulista (Unesp)Royal Museum Cent AfricaAdamawa State UnivUniv Hosp JenaCarvalho, Pedro C.Oliveira, Ezequiel A. deBertollo, Luiz A. C.Yano, Cassia F.Oliveira, Claudio [UNESP]Decru, EvaJegede, Oladele I.Hatanaka, TerumiLiehr, ThomasAl-Rikabi, Ahmed B. H.Cioffi, Marcelo de B.2018-11-26T17:42:46Z2018-11-26T17:42:46Z2017-12-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttp://dx.doi.org/10.3389/fgene.2017.00203Frontiers In Genetics. Lausanne: Frontiers Media Sa, v. 8, 12 p., 2017.1664-8021http://hdl.handle.net/11449/16361810.3389/fgene.2017.00203WOS:000417740600002WOS000417740600002.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers In Genetics2,274info:eu-repo/semantics/openAccess2024-01-17T06:30:52Zoai:repositorio.unesp.br:11449/163618Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:18:44.488902Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
First Chromosomal Analysis in Hepsetidae (Actinopterygii, Characiformes): Insights into Relationship between African and Neotropical Fish Groups |
title |
First Chromosomal Analysis in Hepsetidae (Actinopterygii, Characiformes): Insights into Relationship between African and Neotropical Fish Groups |
spellingShingle |
First Chromosomal Analysis in Hepsetidae (Actinopterygii, Characiformes): Insights into Relationship between African and Neotropical Fish Groups Carvalho, Pedro C. fishes molecular cytogenetics chromosomal painting comparative genomic hybridization (CGH) karyotype evolution |
title_short |
First Chromosomal Analysis in Hepsetidae (Actinopterygii, Characiformes): Insights into Relationship between African and Neotropical Fish Groups |
title_full |
First Chromosomal Analysis in Hepsetidae (Actinopterygii, Characiformes): Insights into Relationship between African and Neotropical Fish Groups |
title_fullStr |
First Chromosomal Analysis in Hepsetidae (Actinopterygii, Characiformes): Insights into Relationship between African and Neotropical Fish Groups |
title_full_unstemmed |
First Chromosomal Analysis in Hepsetidae (Actinopterygii, Characiformes): Insights into Relationship between African and Neotropical Fish Groups |
title_sort |
First Chromosomal Analysis in Hepsetidae (Actinopterygii, Characiformes): Insights into Relationship between African and Neotropical Fish Groups |
author |
Carvalho, Pedro C. |
author_facet |
Carvalho, Pedro C. Oliveira, Ezequiel A. de Bertollo, Luiz A. C. Yano, Cassia F. Oliveira, Claudio [UNESP] Decru, Eva Jegede, Oladele I. Hatanaka, Terumi Liehr, Thomas Al-Rikabi, Ahmed B. H. Cioffi, Marcelo de B. |
author_role |
author |
author2 |
Oliveira, Ezequiel A. de Bertollo, Luiz A. C. Yano, Cassia F. Oliveira, Claudio [UNESP] Decru, Eva Jegede, Oladele I. Hatanaka, Terumi Liehr, Thomas Al-Rikabi, Ahmed B. H. Cioffi, Marcelo de B. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Estado Educ Mato Grosso Seduc MT Universidade Estadual Paulista (Unesp) Royal Museum Cent Africa Adamawa State Univ Univ Hosp Jena |
dc.contributor.author.fl_str_mv |
Carvalho, Pedro C. Oliveira, Ezequiel A. de Bertollo, Luiz A. C. Yano, Cassia F. Oliveira, Claudio [UNESP] Decru, Eva Jegede, Oladele I. Hatanaka, Terumi Liehr, Thomas Al-Rikabi, Ahmed B. H. Cioffi, Marcelo de B. |
dc.subject.por.fl_str_mv |
fishes molecular cytogenetics chromosomal painting comparative genomic hybridization (CGH) karyotype evolution |
topic |
fishes molecular cytogenetics chromosomal painting comparative genomic hybridization (CGH) karyotype evolution |
description |
Hepsetidae is a small fish family with only the genus Hepsetus, with six described species distributed throughout the South, Central and Western regions of Africa, showing a close relationship with the Alestidae and some Neotropical fish families. However, no cytogenetic information is available for both Hepsetidae and Alestidae species, thus preventing any evolutionary comparative studies at the chromosomal level. In the present study, we are providing new cytogenetic data for Hepsetus odoe, including the standard karyotype, C-banding, repetitive DNAs mapping, comparative genomic hybridization (CGH) and whole chromosome painting (WCP), providing chromosomal patterns and subsidies for comparative cytogenetics with other characiform families. Both males and females H. odoe have 2n = 58 chromosomes (10m + 28sm + 20st/a), with most of the C-band positive heterochromatin localized in the centromeric and subtelomeric regions. Only one pair of chromosomes bears proximal 5S rDNA sites in the short arms, contrasting with the 18S rDNA sequences which are located in the terminal regions of four chromosome pairs. Clear interstitial hybridization signals are evidenced for the U1 and U2 snDNA probes, but in only one and two chromosome pairs, respectively. Microsatellite motifs are widely distributed in the karyotype, with exception for the (CGG)(10), (GAA)(10) and (GAG)(10) probes, which highlight conspicuous interstitial signals on an unique pair of chromosomes. Comparative data from conventional and molecular cytogenetics, including CGH and WCP experiments, indicate that H. odoe and some Erythrinidae species, particularly Erythrinus erythrinus, share similar chromosomal sequences suggesting some relatedness among them, although bearing genomic specificities in view of their divergent evolutionary histories. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-12 2018-11-26T17:42:46Z 2018-11-26T17:42:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fgene.2017.00203 Frontiers In Genetics. Lausanne: Frontiers Media Sa, v. 8, 12 p., 2017. 1664-8021 http://hdl.handle.net/11449/163618 10.3389/fgene.2017.00203 WOS:000417740600002 WOS000417740600002.pdf |
url |
http://dx.doi.org/10.3389/fgene.2017.00203 http://hdl.handle.net/11449/163618 |
identifier_str_mv |
Frontiers In Genetics. Lausanne: Frontiers Media Sa, v. 8, 12 p., 2017. 1664-8021 10.3389/fgene.2017.00203 WOS:000417740600002 WOS000417740600002.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers In Genetics 2,274 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
12 application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media Sa |
publisher.none.fl_str_mv |
Frontiers Media Sa |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129506703048704 |