Aspectos epidemiológicos e manejo integrado do brusone do trigo
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) |
Texto Completo: | http://tede.upf.br:8080/jspui/handle/tede/1746 |
Resumo: | The emergence of blast pathogen (Magnaporthe oryzae) infecting wheat (Triticum aestivum L.) is relatively recent in Brazil. The studies published so far have indicated the occurrence of a genetic variability and virulence variability between the M. oryzae populations that are infecting wheat in Brazil. Seedlings and detached heads from 11 wheat cultivars and one barley lineage (T & C) were used in experiments conducted under controlled conditions in which individualized conidial suspension of 89 M. oryzae isolates and two M. grisea isolates were used in inoculation procedures. Presence of the 2NS chromosomal segment in the T & C genotypes was determined via PCR. It was verified that M. oryzae virulence groups are widely distributed in the country and that, due to the high resistance to blast demonstrated, Santa Fe wheat cultivar plays a prominent role as a source of resistance to the disease. In addition, it was found that the evaluation of wheat M. oryzae isolates for virulence generates a very distinct classification patterns depending on whether the object of infection analysis is leaf or head. To monitor the saprotrophic development of M. oryzae on wheat debris and explore the importance of crop residues as a source of inoculum, wheat cultivars BRS 229 and Anahuac 75, moderately and highly susceptible to the disease, respectively, were inoculated with a spore suspension of 10 -5 conidia mL -1 using an aggressive (Py 12.1.209) and a less aggressive (Py 12.1.132) isolate. At maturity, a portion of leaves, stems and spikes were detached from plants, and a group of ten lesions were randomly selected and marked on each type of plant organ. The air -dried plant organs were placed separately inside bags and exposed outside. Each 14 days, samples were taken from the field and tested for sporulation. Sporulation of the fungus was observed on the wheat residues for up to five months. For managing blast disease in wheat, the chemical control is the main option, due to absence of resistance wheat cu ltivars, but it needs to improve wheat head coverage. The experiments were arranged in a randomized block design in a 4x2 factorial scheme, four spray nozzles and two adjuvants, under three replications. The fungicides were sprayed 24 hours before the wheat heads inoculation. In the greenhouse experiment were evaluated the coverage quality by four different spray nozzles in artificial wheat heads surrounded by a hydrosensitive cards, the head blast severity - SEVE and the number of infection points on the rachis – NPI. In the greenhouse experiment, the lowest values to SEVE and NPI were found in the treatment T9 (mancozeb + Break-Thru + Grip: TJ60 10002-60 o ). In field conditions the wheat blast incidence - INC and head blast severity - SEVE were evaluated. The treatment with mancozeb (T9) showed the lowest averages for both disease variables, however, treatment T9 did not differ statistically of treatment T8 for INC variable, for the variable SEVE, treatment T9 not differ statistically of treatments that Break-Thru® adjuvant was added in the spray solution (T5,T6,T7 e T8). |
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Boller, Walter20893140015http://lattes.cnpq.br/849076613195441802644503027http://lattes.cnpq.br/1066717681138634Pizolotto, Carlos Augusto2019-07-10T17:43:26Z2019-02-19PIZOLOTTO, Carlos Augusto. Aspectos epidemiológicos e manejo integrado do brusone do trigo. 2019. 167 f. Tese (Doutorado em Agronomia) - Universidade de Passo Fundo, Passo Fundo, RS, 2019.http://tede.upf.br:8080/jspui/handle/tede/1746The emergence of blast pathogen (Magnaporthe oryzae) infecting wheat (Triticum aestivum L.) is relatively recent in Brazil. The studies published so far have indicated the occurrence of a genetic variability and virulence variability between the M. oryzae populations that are infecting wheat in Brazil. Seedlings and detached heads from 11 wheat cultivars and one barley lineage (T & C) were used in experiments conducted under controlled conditions in which individualized conidial suspension of 89 M. oryzae isolates and two M. grisea isolates were used in inoculation procedures. Presence of the 2NS chromosomal segment in the T & C genotypes was determined via PCR. It was verified that M. oryzae virulence groups are widely distributed in the country and that, due to the high resistance to blast demonstrated, Santa Fe wheat cultivar plays a prominent role as a source of resistance to the disease. In addition, it was found that the evaluation of wheat M. oryzae isolates for virulence generates a very distinct classification patterns depending on whether the object of infection analysis is leaf or head. To monitor the saprotrophic development of M. oryzae on wheat debris and explore the importance of crop residues as a source of inoculum, wheat cultivars BRS 229 and Anahuac 75, moderately and highly susceptible to the disease, respectively, were inoculated with a spore suspension of 10 -5 conidia mL -1 using an aggressive (Py 12.1.209) and a less aggressive (Py 12.1.132) isolate. At maturity, a portion of leaves, stems and spikes were detached from plants, and a group of ten lesions were randomly selected and marked on each type of plant organ. The air -dried plant organs were placed separately inside bags and exposed outside. Each 14 days, samples were taken from the field and tested for sporulation. Sporulation of the fungus was observed on the wheat residues for up to five months. For managing blast disease in wheat, the chemical control is the main option, due to absence of resistance wheat cu ltivars, but it needs to improve wheat head coverage. The experiments were arranged in a randomized block design in a 4x2 factorial scheme, four spray nozzles and two adjuvants, under three replications. The fungicides were sprayed 24 hours before the wheat heads inoculation. In the greenhouse experiment were evaluated the coverage quality by four different spray nozzles in artificial wheat heads surrounded by a hydrosensitive cards, the head blast severity - SEVE and the number of infection points on the rachis – NPI. In the greenhouse experiment, the lowest values to SEVE and NPI were found in the treatment T9 (mancozeb + Break-Thru + Grip: TJ60 10002-60 o ). In field conditions the wheat blast incidence - INC and head blast severity - SEVE were evaluated. The treatment with mancozeb (T9) showed the lowest averages for both disease variables, however, treatment T9 did not differ statistically of treatment T8 for INC variable, for the variable SEVE, treatment T9 not differ statistically of treatments that Break-Thru® adjuvant was added in the spray solution (T5,T6,T7 e T8).A emergência do patógeno causador da brusone (Magnaporthe oryzae) infectando a cultura do trigo (Triticum aestivum L.), é relativamente recente no Brasil. Os estudos publicados até o momento indicaram a ocorrência de variabilidade genética e de virulência entre as populações de M. oryzae infectantes do trigo no Brasil. Plântulas e espigas destacadas de 11 genótipos de trigo e um de cevada (T&C) foram utilizados em experimentos conduzidos em condições controlada, nos quais suspensões individualizadas de conídios de 89 isolados de M. oryzae e dois isolados de M. grisea foram utilizadas em procedimentos de inoculação. A presença do segmento cromossomal 2NS nos genótipos T&C foi determinada via PCR. Verificou-se que grupos de virulência de M. oryzae estão amplamente distribuídos no país e que, devido à alta resistência à brusone demonstrada, a cultivar de trigo Santa Fé assume um papel de destaque como fonte de resistência à doença. A avaliação de isolados quanto à virulência gerou padrões de classificação muito distintos quanto as infecções em folhas e espigas. Para monitorar a sobrevivência saprofítica de M. oryzae em restos de trigo e explorar a importância dos resíduos culturais como fonte de inóculo, as cultivares de trigo Anahuac 75 e BRS 229 foram inoculadas com uma suspensão de esporos de 100.000 conídios mL-1, sendo utilizado um isolado mais virulento (Py 12.1.209) e um menos virulento (Py 12.1.132). Após a manifestação dos sintomas, as lesões foram circundadas em vermelho, com a utilização de pincel atômico. Posteriormente foram destacados folhas, colmos e espigas de cada planta, sendo esses acondicionados em sacos de nylon separadamente e distribuídos no campo. A cada 14 dias, foram coletadas amostras de cada material, e em laboratório as lesões marcadas foram examinadas em microscópio óptico para verificar a presença de conidióforos e conídios de M. oryzae. A esporulação de M. oryzae foi observada nos resíduos de trigo por até cinco meses. Em relação ao manejo da doença na cultura do trigo, o controle químico ainda é a principal estratégia, mas necessita de estudos que visem a melhor cobertura da espiga. Os experimentos foram conduzidos em delineamento de blocos casualizados em esquema fatorial 4x2, com quatro pontas/bicos de pulverização e dois adjuvantes, sob três repetições. No experimento em casa-de-vegetação foram avaliados a qualidade de cobertura proporcionada por quatro diferentes pontas/bicos de pulverização em espigas artificiais de trigo envoltas por cartão hidrossensível, a severidade da doença nas espigas - SEVE e o número de pontos de infecção na ráquis NPI. No experimento em casa-de-vegetação as menores médias de SEVE e NPI foram encontradas no tratamento T9 (mancozebe + Break-Thru + Grip ponta: TJ60 10002 -60o). Em condições de campo foram avaliadas a incidência INC e a severidade da doença nas espigas SEVE. O tratamento com mancozebe (T9) apresentou as menores médias para as duas variáveis da doença, no entanto, sem diferir de T8 para a variável INC, e não diferindo dos tratamentos onde foi adicionado o adjuvante Break-Thru® (T5, T6, T7 e T8), para a variável SEVE.Submitted by Aline Rezende (alinerezende@upf.br) on 2019-07-10T17:43:26Z No. of bitstreams: 1 2019CarlosAugustoPizolotto.pdf: 11164129 bytes, checksum: 9d5994ea166d6a7107d3056e30ea8001 (MD5)Made available in DSpace on 2019-07-10T17:43:26Z (GMT). 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dc.title.por.fl_str_mv |
Aspectos epidemiológicos e manejo integrado do brusone do trigo |
dc.title.alternative.eng.fl_str_mv |
Epidemiological aspects and integrated management of wheat blast |
title |
Aspectos epidemiológicos e manejo integrado do brusone do trigo |
spellingShingle |
Aspectos epidemiológicos e manejo integrado do brusone do trigo Pizolotto, Carlos Augusto Trigo Trigo - Doenças e pragas Brusone CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Aspectos epidemiológicos e manejo integrado do brusone do trigo |
title_full |
Aspectos epidemiológicos e manejo integrado do brusone do trigo |
title_fullStr |
Aspectos epidemiológicos e manejo integrado do brusone do trigo |
title_full_unstemmed |
Aspectos epidemiológicos e manejo integrado do brusone do trigo |
title_sort |
Aspectos epidemiológicos e manejo integrado do brusone do trigo |
author |
Pizolotto, Carlos Augusto |
author_facet |
Pizolotto, Carlos Augusto |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Boller, Walter |
dc.contributor.advisor1ID.fl_str_mv |
20893140015 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8490766131954418 |
dc.contributor.authorID.fl_str_mv |
02644503027 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1066717681138634 |
dc.contributor.author.fl_str_mv |
Pizolotto, Carlos Augusto |
contributor_str_mv |
Boller, Walter |
dc.subject.por.fl_str_mv |
Trigo Trigo - Doenças e pragas Brusone |
topic |
Trigo Trigo - Doenças e pragas Brusone CIENCIAS AGRARIAS::AGRONOMIA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::AGRONOMIA |
description |
The emergence of blast pathogen (Magnaporthe oryzae) infecting wheat (Triticum aestivum L.) is relatively recent in Brazil. The studies published so far have indicated the occurrence of a genetic variability and virulence variability between the M. oryzae populations that are infecting wheat in Brazil. Seedlings and detached heads from 11 wheat cultivars and one barley lineage (T & C) were used in experiments conducted under controlled conditions in which individualized conidial suspension of 89 M. oryzae isolates and two M. grisea isolates were used in inoculation procedures. Presence of the 2NS chromosomal segment in the T & C genotypes was determined via PCR. It was verified that M. oryzae virulence groups are widely distributed in the country and that, due to the high resistance to blast demonstrated, Santa Fe wheat cultivar plays a prominent role as a source of resistance to the disease. In addition, it was found that the evaluation of wheat M. oryzae isolates for virulence generates a very distinct classification patterns depending on whether the object of infection analysis is leaf or head. To monitor the saprotrophic development of M. oryzae on wheat debris and explore the importance of crop residues as a source of inoculum, wheat cultivars BRS 229 and Anahuac 75, moderately and highly susceptible to the disease, respectively, were inoculated with a spore suspension of 10 -5 conidia mL -1 using an aggressive (Py 12.1.209) and a less aggressive (Py 12.1.132) isolate. At maturity, a portion of leaves, stems and spikes were detached from plants, and a group of ten lesions were randomly selected and marked on each type of plant organ. The air -dried plant organs were placed separately inside bags and exposed outside. Each 14 days, samples were taken from the field and tested for sporulation. Sporulation of the fungus was observed on the wheat residues for up to five months. For managing blast disease in wheat, the chemical control is the main option, due to absence of resistance wheat cu ltivars, but it needs to improve wheat head coverage. The experiments were arranged in a randomized block design in a 4x2 factorial scheme, four spray nozzles and two adjuvants, under three replications. The fungicides were sprayed 24 hours before the wheat heads inoculation. In the greenhouse experiment were evaluated the coverage quality by four different spray nozzles in artificial wheat heads surrounded by a hydrosensitive cards, the head blast severity - SEVE and the number of infection points on the rachis – NPI. In the greenhouse experiment, the lowest values to SEVE and NPI were found in the treatment T9 (mancozeb + Break-Thru + Grip: TJ60 10002-60 o ). In field conditions the wheat blast incidence - INC and head blast severity - SEVE were evaluated. The treatment with mancozeb (T9) showed the lowest averages for both disease variables, however, treatment T9 did not differ statistically of treatment T8 for INC variable, for the variable SEVE, treatment T9 not differ statistically of treatments that Break-Thru® adjuvant was added in the spray solution (T5,T6,T7 e T8). |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-07-10T17:43:26Z |
dc.date.issued.fl_str_mv |
2019-02-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
PIZOLOTTO, Carlos Augusto. Aspectos epidemiológicos e manejo integrado do brusone do trigo. 2019. 167 f. Tese (Doutorado em Agronomia) - Universidade de Passo Fundo, Passo Fundo, RS, 2019. |
dc.identifier.uri.fl_str_mv |
http://tede.upf.br:8080/jspui/handle/tede/1746 |
identifier_str_mv |
PIZOLOTTO, Carlos Augusto. Aspectos epidemiológicos e manejo integrado do brusone do trigo. 2019. 167 f. Tese (Doutorado em Agronomia) - Universidade de Passo Fundo, Passo Fundo, RS, 2019. |
url |
http://tede.upf.br:8080/jspui/handle/tede/1746 |
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por |
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por |
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5624066117035054290 |
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500 500 600 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade de Passo Fundo |
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Programa de Pós-Graduação em Agronomia |
dc.publisher.initials.fl_str_mv |
UPF |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Faculdade de Agronomia e Medicina Veterinária – FAMV |
publisher.none.fl_str_mv |
Universidade de Passo Fundo |
dc.source.none.fl_str_mv |
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