Desenvolvimento de um processo integrado de produção e extração de queratinases por Aspergillus spp utilizando sistema de duas fases aquosas

• Autor(a) principal: CORREIA, Patyanne Carvalho
• Data de Publicação: 2013
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRPE
• Texto Completo: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4654
• topic: Queratinases; Aspergillus; Produção de enzimas; Extração de enzimas; keratinases; Production of enzymes; Extraction of enzymes; CIENCIAS AGRARIAS::MEDICINA VETERINARIA

Keratinase are proteolytic enzymes whose substrate are a group of fibers and insoluble proteins denominate keratin. Some several micro-organisms such as fungi are capable to produce keratinase and to hydrolyze disulfide bonds present in keratinous substrates. The aim of this study was to select a producer keratinase and develop an integrated system which combines production, extraction and purification of keratinase by extractive fermentation in aqueous two-phase system (ATPS), prepared with polyethylene glycol (PEG) and citrate salts. Were observed the fermentation with Aspergillus sp SIS 11 was the higher producer of keratinases (7.65 U / mL), which the best conditions for enzyme production showed feather 0.5% o and pH 9.0. An ATPS which is composed of 20% (w/w) PEG 400 molar mass (g/mol), pH 8.0, 20% (w / w) sodium citrate, provided the best conditions for extractive keratinase production. Promoting greater (purification) 7.41, partition coefficient 0.74 and enzyme recovery 503.6%. In extractive fermentation enzyme produced presented partition coefficient K = 1.39 and enzyme recovery 65.08%. The studied strain was effective for enzyme production and biotechnological potential to use in animal feed.