Uso do meio Roswell Park Memorial Institute (RPMI) como conservante de córneas de camundongos swiss
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5626 |
Resumo: | An important function of corneal endothelial cells is to provide barrier and pump to maintain tissue transparency. Therefore the purpose of this study was to investigate the RPMI tissue culture medium for mouse cornea preservation kept under refrigeration. In order to evaluate endothelium cells integrity and shape, corneas were processed, immediately after removal from medium, for light and scanning electron microscopy. Corneas became unavailable as of day 4 of storage, exhibiting morphological alterations, with cellular death characteristics and increased stromal thickness, indicating severe edema. It was concluded that RPMI medium is not viable to preserve corneas for penetrating keratoplasty, nevertheless, we believe that it may be used as a preservation medium for corneal lamellar grafts because it is maintained sterile. |
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SÁ, Fabrício Bezerra deEVÊNCIO NETO, JoaquimMENDONÇA, Fábio de SouzaMEDEIROS, Juliana Pinto dehttp://lattes.cnpq.br/6264181523688475LIRA, Fernando José Xavier de2016-10-07T12:50:23Z2012-02-29LIRA, Fernando José Xavier de. Uso do meio Roswell Park Memorial Institute (RPMI) como conservante de córneas de camundongos swiss. 2012. 30 f. Dissertação (Programa de Pós-Graduação em Ciência Veterinária) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5626An important function of corneal endothelial cells is to provide barrier and pump to maintain tissue transparency. Therefore the purpose of this study was to investigate the RPMI tissue culture medium for mouse cornea preservation kept under refrigeration. In order to evaluate endothelium cells integrity and shape, corneas were processed, immediately after removal from medium, for light and scanning electron microscopy. Corneas became unavailable as of day 4 of storage, exhibiting morphological alterations, with cellular death characteristics and increased stromal thickness, indicating severe edema. It was concluded that RPMI medium is not viable to preserve corneas for penetrating keratoplasty, nevertheless, we believe that it may be used as a preservation medium for corneal lamellar grafts because it is maintained sterile.As células do endotélio corneal apresentam a importante função de bomba e barreira, mantendo assim a córnea transparente.. Portanto, o propósito deste estudo foi investigar o meio de cultura de tecidos RPMI como forma de preservação de córneas de camundongos mantidas sob refrigeração. Imediatamente após a retirada do meio de preservação, as córneas foram processadas para microscopia de luz e eletrônica de varredura para análise da integridade e forma da célula endotelial. As córneas tornaram-se inviáveis a partir do dia 4 de conservação, apresentando alterações morfológicas, sinais de morte celular e espessura estromal aumentada, indicando edema importante. Concluiu-se que o meio RPMI não é viável pra conservação de córneas com a finalidade de ceratoplastia penetrante, embora, acreditamos que por se manter estéril poderá ser usado para enxertos lamelares.Submitted by (edna.saturno@ufrpe.br) on 2016-10-07T12:50:23Z No. of bitstreams: 1 Fernando Jose Xavier de Lira.pdf: 728148 bytes, checksum: 8e97658a43081f68d2680e041cb05bf2 (MD5)Made available in DSpace on 2016-10-07T12:50:23Z (GMT). No. of bitstreams: 1 Fernando Jose Xavier de Lira.pdf: 728148 bytes, checksum: 8e97658a43081f68d2680e041cb05bf2 (MD5) Previous issue date: 2012-02-29Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Ciência VeterináriaUFRPEBrasilDepartamento de Medicina VeterináriaCórneaEndotélio cornealPreservação de córneaMeios de culturaCorneal endothelialCorneal preservationCulture mediumCIENCIAS AGRARIAS::MEDICINA VETERINARIAUso do meio Roswell Park Memorial Institute (RPMI) como conservante de córneas de camundongos swissinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-3061482854177903105600600600600-30202105637636167804536702642350173192075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5626/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51ORIGINALFernando Jose Xavier de Lira.pdfFernando Jose Xavier de Lira.pdfapplication/pdf728148http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5626/2/Fernando+Jose+Xavier+de+Lira.pdf8e97658a43081f68d2680e041cb05bf2MD52tede2/56262016-10-07 09:50:23.572oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:33:18.383827Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Uso do meio Roswell Park Memorial Institute (RPMI) como conservante de córneas de camundongos swiss |
title |
Uso do meio Roswell Park Memorial Institute (RPMI) como conservante de córneas de camundongos swiss |
spellingShingle |
Uso do meio Roswell Park Memorial Institute (RPMI) como conservante de córneas de camundongos swiss LIRA, Fernando José Xavier de Córnea Endotélio corneal Preservação de córnea Meios de cultura Corneal endothelial Corneal preservation Culture medium CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Uso do meio Roswell Park Memorial Institute (RPMI) como conservante de córneas de camundongos swiss |
title_full |
Uso do meio Roswell Park Memorial Institute (RPMI) como conservante de córneas de camundongos swiss |
title_fullStr |
Uso do meio Roswell Park Memorial Institute (RPMI) como conservante de córneas de camundongos swiss |
title_full_unstemmed |
Uso do meio Roswell Park Memorial Institute (RPMI) como conservante de córneas de camundongos swiss |
title_sort |
Uso do meio Roswell Park Memorial Institute (RPMI) como conservante de córneas de camundongos swiss |
author |
LIRA, Fernando José Xavier de |
author_facet |
LIRA, Fernando José Xavier de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
SÁ, Fabrício Bezerra de |
dc.contributor.referee1.fl_str_mv |
EVÊNCIO NETO, Joaquim |
dc.contributor.referee2.fl_str_mv |
MENDONÇA, Fábio de Souza |
dc.contributor.referee3.fl_str_mv |
MEDEIROS, Juliana Pinto de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6264181523688475 |
dc.contributor.author.fl_str_mv |
LIRA, Fernando José Xavier de |
contributor_str_mv |
SÁ, Fabrício Bezerra de EVÊNCIO NETO, Joaquim MENDONÇA, Fábio de Souza MEDEIROS, Juliana Pinto de |
dc.subject.por.fl_str_mv |
Córnea Endotélio corneal Preservação de córnea Meios de cultura |
topic |
Córnea Endotélio corneal Preservação de córnea Meios de cultura Corneal endothelial Corneal preservation Culture medium CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Corneal endothelial Corneal preservation Culture medium |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
An important function of corneal endothelial cells is to provide barrier and pump to maintain tissue transparency. Therefore the purpose of this study was to investigate the RPMI tissue culture medium for mouse cornea preservation kept under refrigeration. In order to evaluate endothelium cells integrity and shape, corneas were processed, immediately after removal from medium, for light and scanning electron microscopy. Corneas became unavailable as of day 4 of storage, exhibiting morphological alterations, with cellular death characteristics and increased stromal thickness, indicating severe edema. It was concluded that RPMI medium is not viable to preserve corneas for penetrating keratoplasty, nevertheless, we believe that it may be used as a preservation medium for corneal lamellar grafts because it is maintained sterile. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-02-29 |
dc.date.accessioned.fl_str_mv |
2016-10-07T12:50:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
LIRA, Fernando José Xavier de. Uso do meio Roswell Park Memorial Institute (RPMI) como conservante de córneas de camundongos swiss. 2012. 30 f. Dissertação (Programa de Pós-Graduação em Ciência Veterinária) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5626 |
identifier_str_mv |
LIRA, Fernando José Xavier de. Uso do meio Roswell Park Memorial Institute (RPMI) como conservante de córneas de camundongos swiss. 2012. 30 f. Dissertação (Programa de Pós-Graduação em Ciência Veterinária) - Universidade Federal Rural de Pernambuco, Recife. |
url |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5626 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-3061482854177903105 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
-3020210563763616780 |
dc.relation.cnpq.fl_str_mv |
453670264235017319 |
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2075167498588264571 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural de Pernambuco |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciência Veterinária |
dc.publisher.initials.fl_str_mv |
UFRPE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Departamento de Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal Rural de Pernambuco |
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Biblioteca Digital de Teses e Dissertações da UFRPE |
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