In vitro remineralization of primary teeth with a mineralization-promoting peptide containing dental varnish
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/190456 |
Resumo: | Mineralization-promoting peptides are attractive candidates for new remineralization systems. In previous studies, peptides have been applied as aqueous solutions, which is not a clinically relevant form. Objective: This study aims to investigate the efficiency of a mineralization-promoting peptide, applied in varnish, on remineralizing artificial caries on primary teeth. Methodology: 55 primary molars were collected. Specimens were immersed in a demineralizing solution for 7 days and then, divided into 7 groups: Baseline: No-remineralization, Placebo: Blank colophony, F: Colophony 5% fluoride, P: Colophony 10% peptide, P+F: Colophony 5% fluoride and 10% peptide, Embrace: Embrace™ varnish, Durashield: Durashield™ varnish. A mixture of 35% w/v colophony varnishes were prepared in ethanol and applied accordingly. Specimens were immersed in a remineralization solution for 4 weeks and it was evaluated using PLM and SEM. Lesion depth reduction was examined by one-way ANOVA. Results: There was no significant difference in mean lesion depths between baseline (147.04±10.18 µm) and placebo groups (139.73±14.92 µm), between F (120.95±12.23 µm) and Durashield (113.47±14.36 µm) groups and between P (81.79±23.15 µm) and Embrace (90.26±17.72 µm) groups. Lesion depth for the P+F group (66.95±10.59 µm) was significantly higher compared to all other groups. All groups contained samples with subsurface demineralized regions. Number of subsurface demineralized regions were higher in fluoride-containing groups. Conclusions: We conclude that the mineralization-promoting peptide (MPP3) is effective in this in vitro study and the peptide shows benefits over fluoride as it yields less subsurface demineralized regions. |
id |
USP-17_5e4a43762af68e2b1d90a8221cd863e6 |
---|---|
oai_identifier_str |
oai:revistas.usp.br:article/190456 |
network_acronym_str |
USP-17 |
network_name_str |
Journal of applied oral science (Online) |
repository_id_str |
|
spelling |
In vitro remineralization of primary teeth with a mineralization-promoting peptide containing dental varnishRemineralizationMineralization-promoting peptideVarnishPLMSEMMineralization-promoting peptides are attractive candidates for new remineralization systems. In previous studies, peptides have been applied as aqueous solutions, which is not a clinically relevant form. Objective: This study aims to investigate the efficiency of a mineralization-promoting peptide, applied in varnish, on remineralizing artificial caries on primary teeth. Methodology: 55 primary molars were collected. Specimens were immersed in a demineralizing solution for 7 days and then, divided into 7 groups: Baseline: No-remineralization, Placebo: Blank colophony, F: Colophony 5% fluoride, P: Colophony 10% peptide, P+F: Colophony 5% fluoride and 10% peptide, Embrace: Embrace™ varnish, Durashield: Durashield™ varnish. A mixture of 35% w/v colophony varnishes were prepared in ethanol and applied accordingly. Specimens were immersed in a remineralization solution for 4 weeks and it was evaluated using PLM and SEM. Lesion depth reduction was examined by one-way ANOVA. Results: There was no significant difference in mean lesion depths between baseline (147.04±10.18 µm) and placebo groups (139.73±14.92 µm), between F (120.95±12.23 µm) and Durashield (113.47±14.36 µm) groups and between P (81.79±23.15 µm) and Embrace (90.26±17.72 µm) groups. Lesion depth for the P+F group (66.95±10.59 µm) was significantly higher compared to all other groups. All groups contained samples with subsurface demineralized regions. Number of subsurface demineralized regions were higher in fluoride-containing groups. Conclusions: We conclude that the mineralization-promoting peptide (MPP3) is effective in this in vitro study and the peptide shows benefits over fluoride as it yields less subsurface demineralized regions.Universidade de São Paulo. Faculdade de Odontologia de Bauru2021-09-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/19045610.1590/1678-7757-2020-0259 Journal of Applied Oral Science; Vol. 28 (2020); e20200259Journal of Applied Oral Science; Vol. 28 (2020); e20200259Journal of Applied Oral Science; v. 28 (2020); e202002591678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/190456/175998Copyright (c) 2021 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessGungormus, MustafaTulumbaci, Fatih2021-09-08T13:56:00Zoai:revistas.usp.br:article/190456Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2021-09-08T13:56Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
In vitro remineralization of primary teeth with a mineralization-promoting peptide containing dental varnish |
title |
In vitro remineralization of primary teeth with a mineralization-promoting peptide containing dental varnish |
spellingShingle |
In vitro remineralization of primary teeth with a mineralization-promoting peptide containing dental varnish Gungormus, Mustafa Remineralization Mineralization-promoting peptide Varnish PLM SEM |
title_short |
In vitro remineralization of primary teeth with a mineralization-promoting peptide containing dental varnish |
title_full |
In vitro remineralization of primary teeth with a mineralization-promoting peptide containing dental varnish |
title_fullStr |
In vitro remineralization of primary teeth with a mineralization-promoting peptide containing dental varnish |
title_full_unstemmed |
In vitro remineralization of primary teeth with a mineralization-promoting peptide containing dental varnish |
title_sort |
In vitro remineralization of primary teeth with a mineralization-promoting peptide containing dental varnish |
author |
Gungormus, Mustafa |
author_facet |
Gungormus, Mustafa Tulumbaci, Fatih |
author_role |
author |
author2 |
Tulumbaci, Fatih |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Gungormus, Mustafa Tulumbaci, Fatih |
dc.subject.por.fl_str_mv |
Remineralization Mineralization-promoting peptide Varnish PLM SEM |
topic |
Remineralization Mineralization-promoting peptide Varnish PLM SEM |
description |
Mineralization-promoting peptides are attractive candidates for new remineralization systems. In previous studies, peptides have been applied as aqueous solutions, which is not a clinically relevant form. Objective: This study aims to investigate the efficiency of a mineralization-promoting peptide, applied in varnish, on remineralizing artificial caries on primary teeth. Methodology: 55 primary molars were collected. Specimens were immersed in a demineralizing solution for 7 days and then, divided into 7 groups: Baseline: No-remineralization, Placebo: Blank colophony, F: Colophony 5% fluoride, P: Colophony 10% peptide, P+F: Colophony 5% fluoride and 10% peptide, Embrace: Embrace™ varnish, Durashield: Durashield™ varnish. A mixture of 35% w/v colophony varnishes were prepared in ethanol and applied accordingly. Specimens were immersed in a remineralization solution for 4 weeks and it was evaluated using PLM and SEM. Lesion depth reduction was examined by one-way ANOVA. Results: There was no significant difference in mean lesion depths between baseline (147.04±10.18 µm) and placebo groups (139.73±14.92 µm), between F (120.95±12.23 µm) and Durashield (113.47±14.36 µm) groups and between P (81.79±23.15 µm) and Embrace (90.26±17.72 µm) groups. Lesion depth for the P+F group (66.95±10.59 µm) was significantly higher compared to all other groups. All groups contained samples with subsurface demineralized regions. Number of subsurface demineralized regions were higher in fluoride-containing groups. Conclusions: We conclude that the mineralization-promoting peptide (MPP3) is effective in this in vitro study and the peptide shows benefits over fluoride as it yields less subsurface demineralized regions. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-22 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/190456 10.1590/1678-7757-2020-0259 |
url |
https://www.revistas.usp.br/jaos/article/view/190456 |
identifier_str_mv |
10.1590/1678-7757-2020-0259 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/190456/175998 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 28 (2020); e20200259 Journal of Applied Oral Science; Vol. 28 (2020); e20200259 Journal of Applied Oral Science; v. 28 (2020); e20200259 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221682004131840 |