Influence of human and bovine substrate on the microleakage of two adhesive systems

Detalhes bibliográficos
Autor(a) principal: Almeida,Karoline Guará Brusaca
Data de Publicação: 2009
Outros Autores: Scheibe,Kristine Guará Brusaca Almeida, Oliveira,Ana Emília Figuerêdo, Alves,Cláudia Maria Coêlho, Costa,José Ferreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572009000200004
Resumo: The aim of this study was to evaluate in vitro the marginal sealing of two adhesive systems and to analyze the influence of human and bovine substrates on marginal microleakage in enamel. Rectangular-shaped class V cavities (4 mm wide x 2 mm high x 2 mm deep) were made as follows: 8 cavities were prepared on the buccal and lingual surfaces of the human teeth with margins located on enamel and 16 cavities were prepared on the buccal surfaces of the bovine teeth. The cavities were randomly assigned to 4 groups of 8 cavities according to the adhesive system and substrate: G1 - Prime & Bond 2.1 (Dentsply)/human substrate; G2 - Adhese (Ivoclar/Vivadent)/human substrate; G3 - Prime & Bond 2.1 (Dentsply)/bovine substrate; G4 - Adhese (Ivoclar/Vivadent)/bovine substrate. The cavities were filled with microhybrid composite resin (Fillmagic) and after polishing/finishing procedures, the teeth were subjected to a thermocycling regimen of 500 cycles with 1-min immersions in water at 55° ±2°C and 5° ± 2°C. Next, the teeth were coated with two layers of nail polish to within 1 mm of the margin, submerged in a 50% silver nitrate solution for 2 h, rinsed thoroughly in running tap and immersed in developing solution for 8 h. The restorations were bisected resulting in 16 specimens. Microleakage was observed under a stereomicroscope at x25 and recorded using four-point (0-3) scoring system. The data were analyzed statistically by the Mann Whitney U-test at 5% significance level. Leakage was present in all specimens and there was statistically significant difference between the adhesive systems. Adhese self-etching system showed significantly more leakage in both substrates (human - p= 0.0001 and bovine - p= 0.0031). There was no statistically significant difference between human and bovine substrates for either of the adhesive systems based on different bonding mechanisms (Prime & Bond 2.1 - p= 0.6923 and Adhese - p= 0.6109). Neither of the adhesive systems was capable to completely prevent microleakage and the self-etching system was more susceptible to microleakage.
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spelling Influence of human and bovine substrate on the microleakage of two adhesive systemsToothMicroleakageBovine and human teethSelf-etching adhesivesThe aim of this study was to evaluate in vitro the marginal sealing of two adhesive systems and to analyze the influence of human and bovine substrates on marginal microleakage in enamel. Rectangular-shaped class V cavities (4 mm wide x 2 mm high x 2 mm deep) were made as follows: 8 cavities were prepared on the buccal and lingual surfaces of the human teeth with margins located on enamel and 16 cavities were prepared on the buccal surfaces of the bovine teeth. The cavities were randomly assigned to 4 groups of 8 cavities according to the adhesive system and substrate: G1 - Prime & Bond 2.1 (Dentsply)/human substrate; G2 - Adhese (Ivoclar/Vivadent)/human substrate; G3 - Prime & Bond 2.1 (Dentsply)/bovine substrate; G4 - Adhese (Ivoclar/Vivadent)/bovine substrate. The cavities were filled with microhybrid composite resin (Fillmagic) and after polishing/finishing procedures, the teeth were subjected to a thermocycling regimen of 500 cycles with 1-min immersions in water at 55° ±2°C and 5° ± 2°C. Next, the teeth were coated with two layers of nail polish to within 1 mm of the margin, submerged in a 50% silver nitrate solution for 2 h, rinsed thoroughly in running tap and immersed in developing solution for 8 h. The restorations were bisected resulting in 16 specimens. Microleakage was observed under a stereomicroscope at x25 and recorded using four-point (0-3) scoring system. The data were analyzed statistically by the Mann Whitney U-test at 5% significance level. Leakage was present in all specimens and there was statistically significant difference between the adhesive systems. Adhese self-etching system showed significantly more leakage in both substrates (human - p= 0.0001 and bovine - p= 0.0031). There was no statistically significant difference between human and bovine substrates for either of the adhesive systems based on different bonding mechanisms (Prime & Bond 2.1 - p= 0.6923 and Adhese - p= 0.6109). Neither of the adhesive systems was capable to completely prevent microleakage and the self-etching system was more susceptible to microleakage.Faculdade De Odontologia De Bauru - USP2009-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572009000200004Journal of Applied Oral Science v.17 n.2 2009reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S1678-77572009000200004info:eu-repo/semantics/openAccessAlmeida,Karoline Guará BrusacaScheibe,Kristine Guará Brusaca AlmeidaOliveira,Ana Emília FiguerêdoAlves,Cláudia Maria CoêlhoCosta,José Ferreiraeng2009-01-19T00:00:00Zoai:scielo:S1678-77572009000200004Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2009-01-19T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Influence of human and bovine substrate on the microleakage of two adhesive systems
title Influence of human and bovine substrate on the microleakage of two adhesive systems
spellingShingle Influence of human and bovine substrate on the microleakage of two adhesive systems
Almeida,Karoline Guará Brusaca
Tooth
Microleakage
Bovine and human teeth
Self-etching adhesives
title_short Influence of human and bovine substrate on the microleakage of two adhesive systems
title_full Influence of human and bovine substrate on the microleakage of two adhesive systems
title_fullStr Influence of human and bovine substrate on the microleakage of two adhesive systems
title_full_unstemmed Influence of human and bovine substrate on the microleakage of two adhesive systems
title_sort Influence of human and bovine substrate on the microleakage of two adhesive systems
author Almeida,Karoline Guará Brusaca
author_facet Almeida,Karoline Guará Brusaca
Scheibe,Kristine Guará Brusaca Almeida
Oliveira,Ana Emília Figuerêdo
Alves,Cláudia Maria Coêlho
Costa,José Ferreira
author_role author
author2 Scheibe,Kristine Guará Brusaca Almeida
Oliveira,Ana Emília Figuerêdo
Alves,Cláudia Maria Coêlho
Costa,José Ferreira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Almeida,Karoline Guará Brusaca
Scheibe,Kristine Guará Brusaca Almeida
Oliveira,Ana Emília Figuerêdo
Alves,Cláudia Maria Coêlho
Costa,José Ferreira
dc.subject.por.fl_str_mv Tooth
Microleakage
Bovine and human teeth
Self-etching adhesives
topic Tooth
Microleakage
Bovine and human teeth
Self-etching adhesives
description The aim of this study was to evaluate in vitro the marginal sealing of two adhesive systems and to analyze the influence of human and bovine substrates on marginal microleakage in enamel. Rectangular-shaped class V cavities (4 mm wide x 2 mm high x 2 mm deep) were made as follows: 8 cavities were prepared on the buccal and lingual surfaces of the human teeth with margins located on enamel and 16 cavities were prepared on the buccal surfaces of the bovine teeth. The cavities were randomly assigned to 4 groups of 8 cavities according to the adhesive system and substrate: G1 - Prime & Bond 2.1 (Dentsply)/human substrate; G2 - Adhese (Ivoclar/Vivadent)/human substrate; G3 - Prime & Bond 2.1 (Dentsply)/bovine substrate; G4 - Adhese (Ivoclar/Vivadent)/bovine substrate. The cavities were filled with microhybrid composite resin (Fillmagic) and after polishing/finishing procedures, the teeth were subjected to a thermocycling regimen of 500 cycles with 1-min immersions in water at 55° ±2°C and 5° ± 2°C. Next, the teeth were coated with two layers of nail polish to within 1 mm of the margin, submerged in a 50% silver nitrate solution for 2 h, rinsed thoroughly in running tap and immersed in developing solution for 8 h. The restorations were bisected resulting in 16 specimens. Microleakage was observed under a stereomicroscope at x25 and recorded using four-point (0-3) scoring system. The data were analyzed statistically by the Mann Whitney U-test at 5% significance level. Leakage was present in all specimens and there was statistically significant difference between the adhesive systems. Adhese self-etching system showed significantly more leakage in both substrates (human - p= 0.0001 and bovine - p= 0.0031). There was no statistically significant difference between human and bovine substrates for either of the adhesive systems based on different bonding mechanisms (Prime & Bond 2.1 - p= 0.6923 and Adhese - p= 0.6109). Neither of the adhesive systems was capable to completely prevent microleakage and the self-etching system was more susceptible to microleakage.
publishDate 2009
dc.date.none.fl_str_mv 2009-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572009000200004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572009000200004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1678-77572009000200004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
dc.source.none.fl_str_mv Journal of Applied Oral Science v.17 n.2 2009
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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