Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/3589 |
Resumo: | From a biological standpoint, the best material for reconstruction of bone defects is the autogenous bone graft. However, as tissue healing is affected under diabetic conditions, major changes might take place in the revascularization, incorporation, replacement and remodeling phases of the grafted area. The purpose of this study was to assess the bone healing process in surgical wounds prepared in tibiae of diabetic rats and filled with autogenous bone. Forty male rats (Rattus norvegicus albinus, Wistar) were randomly assigned to receive an endovenous injection (penile vein) of either citrate buffer solution (Group 1 - control; n=20) or streptozotocin dissolved in citrate buffer solution (35 mg/kg) to induce diabetes (Group 2 - diabetic; n=20). After determination of glycemia, the animals were anesthetized and the anterolateral regions of the tibiae of both limbs were shaved, antisepsis was performed and longitudinal incisions were made in each limb. The tibiae were exposed and two 2mm-diameter surgical cavities were prepared: one in the right limb, filled with particulate autogenous bone and the other in the left limb, filled with blood clot. The animals were euthanized at 10 and 30 postoperative days. The anatomic pieces were obtained, submitted to laboratory processing and sections were stained by hematoxylin and eosin and Masson's Trichrome for histomorphologic and histometric analyses. In both groups, the wounds filled with autogenous bone graft showed better results than those filled with blood clot. The control group showed higher new bone formation in wounds filled with autogenous bone graft at 30 days than the diabetic group, but without statistical significance. It may be concluded that, in general, the new bone formation occurred with autogenous graft was quantitatively similar between control and diabetic groups and qualitatively better in the control group. |
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Journal of applied oral science (Online) |
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Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats Bone transplantationDiabetes mellitusWound healing From a biological standpoint, the best material for reconstruction of bone defects is the autogenous bone graft. However, as tissue healing is affected under diabetic conditions, major changes might take place in the revascularization, incorporation, replacement and remodeling phases of the grafted area. The purpose of this study was to assess the bone healing process in surgical wounds prepared in tibiae of diabetic rats and filled with autogenous bone. Forty male rats (Rattus norvegicus albinus, Wistar) were randomly assigned to receive an endovenous injection (penile vein) of either citrate buffer solution (Group 1 - control; n=20) or streptozotocin dissolved in citrate buffer solution (35 mg/kg) to induce diabetes (Group 2 - diabetic; n=20). After determination of glycemia, the animals were anesthetized and the anterolateral regions of the tibiae of both limbs were shaved, antisepsis was performed and longitudinal incisions were made in each limb. The tibiae were exposed and two 2mm-diameter surgical cavities were prepared: one in the right limb, filled with particulate autogenous bone and the other in the left limb, filled with blood clot. The animals were euthanized at 10 and 30 postoperative days. The anatomic pieces were obtained, submitted to laboratory processing and sections were stained by hematoxylin and eosin and Masson's Trichrome for histomorphologic and histometric analyses. In both groups, the wounds filled with autogenous bone graft showed better results than those filled with blood clot. The control group showed higher new bone formation in wounds filled with autogenous bone graft at 30 days than the diabetic group, but without statistical significance. It may be concluded that, in general, the new bone formation occurred with autogenous graft was quantitatively similar between control and diabetic groups and qualitatively better in the control group. Universidade de São Paulo. Faculdade de Odontologia de Bauru2008-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/358910.1590/S1678-77572008000500003Journal of Applied Oral Science; Vol. 16 No. 5 (2008); 316-320 Journal of Applied Oral Science; Vol. 16 Núm. 5 (2008); 316-320 Journal of Applied Oral Science; v. 16 n. 5 (2008); 316-320 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3589/4279Copyright (c) 2008 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessEsteves, Jônatas CaldeiraAranega, Alessandra MarcondesBorrasca, Albanir GabrielFattah, Cristiane Mara Ruiz de SouzaGarcia-Júnior, Idelmo Rangel2012-04-27T12:01:32Zoai:revistas.usp.br:article/3589Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T12:01:32Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
title |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
spellingShingle |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats Esteves, Jônatas Caldeira Bone transplantation Diabetes mellitus Wound healing |
title_short |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
title_full |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
title_fullStr |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
title_full_unstemmed |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
title_sort |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
author |
Esteves, Jônatas Caldeira |
author_facet |
Esteves, Jônatas Caldeira Aranega, Alessandra Marcondes Borrasca, Albanir Gabriel Fattah, Cristiane Mara Ruiz de Souza Garcia-Júnior, Idelmo Rangel |
author_role |
author |
author2 |
Aranega, Alessandra Marcondes Borrasca, Albanir Gabriel Fattah, Cristiane Mara Ruiz de Souza Garcia-Júnior, Idelmo Rangel |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Esteves, Jônatas Caldeira Aranega, Alessandra Marcondes Borrasca, Albanir Gabriel Fattah, Cristiane Mara Ruiz de Souza Garcia-Júnior, Idelmo Rangel |
dc.subject.por.fl_str_mv |
Bone transplantation Diabetes mellitus Wound healing |
topic |
Bone transplantation Diabetes mellitus Wound healing |
description |
From a biological standpoint, the best material for reconstruction of bone defects is the autogenous bone graft. However, as tissue healing is affected under diabetic conditions, major changes might take place in the revascularization, incorporation, replacement and remodeling phases of the grafted area. The purpose of this study was to assess the bone healing process in surgical wounds prepared in tibiae of diabetic rats and filled with autogenous bone. Forty male rats (Rattus norvegicus albinus, Wistar) were randomly assigned to receive an endovenous injection (penile vein) of either citrate buffer solution (Group 1 - control; n=20) or streptozotocin dissolved in citrate buffer solution (35 mg/kg) to induce diabetes (Group 2 - diabetic; n=20). After determination of glycemia, the animals were anesthetized and the anterolateral regions of the tibiae of both limbs were shaved, antisepsis was performed and longitudinal incisions were made in each limb. The tibiae were exposed and two 2mm-diameter surgical cavities were prepared: one in the right limb, filled with particulate autogenous bone and the other in the left limb, filled with blood clot. The animals were euthanized at 10 and 30 postoperative days. The anatomic pieces were obtained, submitted to laboratory processing and sections were stained by hematoxylin and eosin and Masson's Trichrome for histomorphologic and histometric analyses. In both groups, the wounds filled with autogenous bone graft showed better results than those filled with blood clot. The control group showed higher new bone formation in wounds filled with autogenous bone graft at 30 days than the diabetic group, but without statistical significance. It may be concluded that, in general, the new bone formation occurred with autogenous graft was quantitatively similar between control and diabetic groups and qualitatively better in the control group. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3589 10.1590/S1678-77572008000500003 |
url |
https://www.revistas.usp.br/jaos/article/view/3589 |
identifier_str_mv |
10.1590/S1678-77572008000500003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3589/4279 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2008 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2008 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 16 No. 5 (2008); 316-320 Journal of Applied Oral Science; Vol. 16 Núm. 5 (2008); 316-320 Journal of Applied Oral Science; v. 16 n. 5 (2008); 316-320 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221673975185408 |