Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses

Detalhes bibliográficos
Autor(a) principal: Arena, Gabriella Dias
Data de Publicação: 2017
Outros Autores: Ramos-González, Pedro Luis, Nunes, Maria Andréia, Jesus, Camila Chabi, Calegario, Renata Faier, Kitajima, Elliot Watanabe, Novelli, Valdenice Moreira, Freitas-Astúa, Juliana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: https://www.revistas.usp.br/sa/article/view/130927
Resumo: Brevipalpus-transmitted viruses (BTV) are a taxonomically diverse group of plant viruses which severely affect a number of major crops. Members of the group can be sub-classified into cytoplasmic (BTV-C) or nuclear type (BTV-N) according to the accumulation sites of virions in the infected plant cells. Both types of BTV produce only local infections near the point of inoculation by viruliferous mites. Features of BTV-plant interactions such as the failure of systemic spread in their natural hosts are poorly understood. In this study we evaluated Arabidopsis thaliana, a model plant commonly used for the study of plant-virus interactions, as an alternative host for BTV. Infection of Arabidopsis with the BTV-N Coffee ringspot virus and Clerodendrum chlorotic spot virus, and the BTV-C Solanum violaefolium ringspot virus, were mediated by viruliferous Brevipalpus mites collected in the wild. Upon infestation, local lesions appeared in 7 to 10 days on leaves of, at least, 80 % of the assayed plants. Presence of viral particles and characteristic cytopathic effects were detected by transmission electron microscopy (TEM) and the viral identities confirmed by specific reverse-transcriptase polymerase chain reaction (RT-PCR) and further amplicon sequencing. The high infection rate and reproducibility of symptoms of the three different viruses assayed validate A. thaliana as a feasible alternative experimental host for BTV.
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spelling Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted virusesClerodendrum chlorotic spot virusCilevirusSolanum violaefolium ringspot virusCoffee ringspot virusDichorhavirusBrevipalpus-transmitted viruses (BTV) are a taxonomically diverse group of plant viruses which severely affect a number of major crops. Members of the group can be sub-classified into cytoplasmic (BTV-C) or nuclear type (BTV-N) according to the accumulation sites of virions in the infected plant cells. Both types of BTV produce only local infections near the point of inoculation by viruliferous mites. Features of BTV-plant interactions such as the failure of systemic spread in their natural hosts are poorly understood. In this study we evaluated Arabidopsis thaliana, a model plant commonly used for the study of plant-virus interactions, as an alternative host for BTV. Infection of Arabidopsis with the BTV-N Coffee ringspot virus and Clerodendrum chlorotic spot virus, and the BTV-C Solanum violaefolium ringspot virus, were mediated by viruliferous Brevipalpus mites collected in the wild. Upon infestation, local lesions appeared in 7 to 10 days on leaves of, at least, 80 % of the assayed plants. Presence of viral particles and characteristic cytopathic effects were detected by transmission electron microscopy (TEM) and the viral identities confirmed by specific reverse-transcriptase polymerase chain reaction (RT-PCR) and further amplicon sequencing. The high infection rate and reproducibility of symptoms of the three different viruses assayed validate A. thaliana as a feasible alternative experimental host for BTV.Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz2017-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/sa/article/view/13092710.1590/1678-992x-2015-0380Scientia Agricola; v. 74 n. 1 (2017); 85-89Scientia Agricola; Vol. 74 Núm. 1 (2017); 85-89Scientia Agricola; Vol. 74 No. 1 (2017); 85-891678-992X0103-9016reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/sa/article/view/130927/127386Copyright (c) 2017 Scientia Agricolainfo:eu-repo/semantics/openAccessArena, Gabriella DiasRamos-González, Pedro LuisNunes, Maria AndréiaJesus, Camila ChabiCalegario, Renata FaierKitajima, Elliot WatanabeNovelli, Valdenice MoreiraFreitas-Astúa, Juliana2017-06-12T11:44:51Zoai:revistas.usp.br:article/130927Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2017-06-12T11:44:51Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses
title Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses
spellingShingle Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses
Arena, Gabriella Dias
Clerodendrum chlorotic spot virus
Cilevirus
Solanum violaefolium ringspot virus
Coffee ringspot virus
Dichorhavirus
title_short Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses
title_full Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses
title_fullStr Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses
title_full_unstemmed Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses
title_sort Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses
author Arena, Gabriella Dias
author_facet Arena, Gabriella Dias
Ramos-González, Pedro Luis
Nunes, Maria Andréia
Jesus, Camila Chabi
Calegario, Renata Faier
Kitajima, Elliot Watanabe
Novelli, Valdenice Moreira
Freitas-Astúa, Juliana
author_role author
author2 Ramos-González, Pedro Luis
Nunes, Maria Andréia
Jesus, Camila Chabi
Calegario, Renata Faier
Kitajima, Elliot Watanabe
Novelli, Valdenice Moreira
Freitas-Astúa, Juliana
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Arena, Gabriella Dias
Ramos-González, Pedro Luis
Nunes, Maria Andréia
Jesus, Camila Chabi
Calegario, Renata Faier
Kitajima, Elliot Watanabe
Novelli, Valdenice Moreira
Freitas-Astúa, Juliana
dc.subject.por.fl_str_mv Clerodendrum chlorotic spot virus
Cilevirus
Solanum violaefolium ringspot virus
Coffee ringspot virus
Dichorhavirus
topic Clerodendrum chlorotic spot virus
Cilevirus
Solanum violaefolium ringspot virus
Coffee ringspot virus
Dichorhavirus
description Brevipalpus-transmitted viruses (BTV) are a taxonomically diverse group of plant viruses which severely affect a number of major crops. Members of the group can be sub-classified into cytoplasmic (BTV-C) or nuclear type (BTV-N) according to the accumulation sites of virions in the infected plant cells. Both types of BTV produce only local infections near the point of inoculation by viruliferous mites. Features of BTV-plant interactions such as the failure of systemic spread in their natural hosts are poorly understood. In this study we evaluated Arabidopsis thaliana, a model plant commonly used for the study of plant-virus interactions, as an alternative host for BTV. Infection of Arabidopsis with the BTV-N Coffee ringspot virus and Clerodendrum chlorotic spot virus, and the BTV-C Solanum violaefolium ringspot virus, were mediated by viruliferous Brevipalpus mites collected in the wild. Upon infestation, local lesions appeared in 7 to 10 days on leaves of, at least, 80 % of the assayed plants. Presence of viral particles and characteristic cytopathic effects were detected by transmission electron microscopy (TEM) and the viral identities confirmed by specific reverse-transcriptase polymerase chain reaction (RT-PCR) and further amplicon sequencing. The high infection rate and reproducibility of symptoms of the three different viruses assayed validate A. thaliana as a feasible alternative experimental host for BTV.
publishDate 2017
dc.date.none.fl_str_mv 2017-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/sa/article/view/130927
10.1590/1678-992x-2015-0380
url https://www.revistas.usp.br/sa/article/view/130927
identifier_str_mv 10.1590/1678-992x-2015-0380
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/sa/article/view/130927/127386
dc.rights.driver.fl_str_mv Copyright (c) 2017 Scientia Agricola
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Scientia Agricola
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
publisher.none.fl_str_mv Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
dc.source.none.fl_str_mv Scientia Agricola; v. 74 n. 1 (2017); 85-89
Scientia Agricola; Vol. 74 Núm. 1 (2017); 85-89
Scientia Agricola; Vol. 74 No. 1 (2017); 85-89
1678-992X
0103-9016
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
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