Relevant proteins for the monitoring of engraftment phases after allogeneic hematopoietic stem cell transplantation
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/213591 |
Resumo: | Introduction: Hematopoietic Stem Cell Transplant (HSCT) has been successfully used as standard therapy for hematological disorders. After conditioning therapy, patients undergoing allogeneic HSCT, present three different phases of engraftment: early pre-engraftment, early post-engraftment, and late engraftment. Severe complications are associated with morbidity, mortality, and malignancies in these phases, which include effects on the oral cavity. Objectives: The changes in the salivary composition after HSCT may contribute to identifying relevant proteins that could map differences among the phases of diseases, driven for personalized diagnostics and therapy. Methods: Unstimulated whole saliva was collected from patients submitted to HSCT. The samples were submitted to trypsin digestion for a Mass spectrometry analysis. MaxQuant processed the Data analysis, and the relevant expressed proteins were subjected to pathway and network analyses. Results: Differences were observed in the most identified proteins, specifically in proteins involved with the regulation of body fluid levels and the mucosal immune response. The heatmap showed a list of proteins exclusively expressed during the different phases of HSCT: HBB, KNG1, HSPA, FGB, APOA1, PFN1, PRTN3, TMSB4X, YWHAZ, CAP1, ACTN1, CLU and ALDOA. Bioinformatics analysis implicated pathways involved in protein processing in the endoplasmic reticulum, complement and coagulation cascades, apoptosis signaling, and cholesterol metabolism. Conclusion: The compositional changes in saliva reflected the three phases of HSCT and demonstrated the usefulness of proteomics and computational approaches as a revolutionary field in diagnostic methods. |
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Clinics |
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Relevant proteins for the monitoring of engraftment phases after allogeneic hematopoietic stem cell transplantationProteomicsHematopoietic stem cell transplantationRelevant proteinsSalivaBiological processIntroduction: Hematopoietic Stem Cell Transplant (HSCT) has been successfully used as standard therapy for hematological disorders. After conditioning therapy, patients undergoing allogeneic HSCT, present three different phases of engraftment: early pre-engraftment, early post-engraftment, and late engraftment. Severe complications are associated with morbidity, mortality, and malignancies in these phases, which include effects on the oral cavity. Objectives: The changes in the salivary composition after HSCT may contribute to identifying relevant proteins that could map differences among the phases of diseases, driven for personalized diagnostics and therapy. Methods: Unstimulated whole saliva was collected from patients submitted to HSCT. The samples were submitted to trypsin digestion for a Mass spectrometry analysis. MaxQuant processed the Data analysis, and the relevant expressed proteins were subjected to pathway and network analyses. Results: Differences were observed in the most identified proteins, specifically in proteins involved with the regulation of body fluid levels and the mucosal immune response. The heatmap showed a list of proteins exclusively expressed during the different phases of HSCT: HBB, KNG1, HSPA, FGB, APOA1, PFN1, PRTN3, TMSB4X, YWHAZ, CAP1, ACTN1, CLU and ALDOA. Bioinformatics analysis implicated pathways involved in protein processing in the endoplasmic reticulum, complement and coagulation cascades, apoptosis signaling, and cholesterol metabolism. Conclusion: The compositional changes in saliva reflected the three phases of HSCT and demonstrated the usefulness of proteomics and computational approaches as a revolutionary field in diagnostic methods.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2022-11-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/21359110.1016/j.clinsp.2022.100134Clinics; Vol. 77 (2022); 100134Clinics; v. 77 (2022); 100134Clinics; Vol. 77 (2022); 1001341980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/213591/195676Copyright (c) 2023 Clinicsinfo:eu-repo/semantics/openAccessSouza, Milena MonteiroCoutinho-Camillo, Cláudia MalheirosPaula, Fabiana Martins dePaula, Fernanda deBologna, Sheyla BatistaLourenço, Silvia Vanessa2023-07-06T13:05:00Zoai:revistas.usp.br:article/213591Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2023-07-06T13:05Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Relevant proteins for the monitoring of engraftment phases after allogeneic hematopoietic stem cell transplantation |
title |
Relevant proteins for the monitoring of engraftment phases after allogeneic hematopoietic stem cell transplantation |
spellingShingle |
Relevant proteins for the monitoring of engraftment phases after allogeneic hematopoietic stem cell transplantation Souza, Milena Monteiro Proteomics Hematopoietic stem cell transplantation Relevant proteins Saliva Biological process |
title_short |
Relevant proteins for the monitoring of engraftment phases after allogeneic hematopoietic stem cell transplantation |
title_full |
Relevant proteins for the monitoring of engraftment phases after allogeneic hematopoietic stem cell transplantation |
title_fullStr |
Relevant proteins for the monitoring of engraftment phases after allogeneic hematopoietic stem cell transplantation |
title_full_unstemmed |
Relevant proteins for the monitoring of engraftment phases after allogeneic hematopoietic stem cell transplantation |
title_sort |
Relevant proteins for the monitoring of engraftment phases after allogeneic hematopoietic stem cell transplantation |
author |
Souza, Milena Monteiro |
author_facet |
Souza, Milena Monteiro Coutinho-Camillo, Cláudia Malheiros Paula, Fabiana Martins de Paula, Fernanda de Bologna, Sheyla Batista Lourenço, Silvia Vanessa |
author_role |
author |
author2 |
Coutinho-Camillo, Cláudia Malheiros Paula, Fabiana Martins de Paula, Fernanda de Bologna, Sheyla Batista Lourenço, Silvia Vanessa |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Souza, Milena Monteiro Coutinho-Camillo, Cláudia Malheiros Paula, Fabiana Martins de Paula, Fernanda de Bologna, Sheyla Batista Lourenço, Silvia Vanessa |
dc.subject.por.fl_str_mv |
Proteomics Hematopoietic stem cell transplantation Relevant proteins Saliva Biological process |
topic |
Proteomics Hematopoietic stem cell transplantation Relevant proteins Saliva Biological process |
description |
Introduction: Hematopoietic Stem Cell Transplant (HSCT) has been successfully used as standard therapy for hematological disorders. After conditioning therapy, patients undergoing allogeneic HSCT, present three different phases of engraftment: early pre-engraftment, early post-engraftment, and late engraftment. Severe complications are associated with morbidity, mortality, and malignancies in these phases, which include effects on the oral cavity. Objectives: The changes in the salivary composition after HSCT may contribute to identifying relevant proteins that could map differences among the phases of diseases, driven for personalized diagnostics and therapy. Methods: Unstimulated whole saliva was collected from patients submitted to HSCT. The samples were submitted to trypsin digestion for a Mass spectrometry analysis. MaxQuant processed the Data analysis, and the relevant expressed proteins were subjected to pathway and network analyses. Results: Differences were observed in the most identified proteins, specifically in proteins involved with the regulation of body fluid levels and the mucosal immune response. The heatmap showed a list of proteins exclusively expressed during the different phases of HSCT: HBB, KNG1, HSPA, FGB, APOA1, PFN1, PRTN3, TMSB4X, YWHAZ, CAP1, ACTN1, CLU and ALDOA. Bioinformatics analysis implicated pathways involved in protein processing in the endoplasmic reticulum, complement and coagulation cascades, apoptosis signaling, and cholesterol metabolism. Conclusion: The compositional changes in saliva reflected the three phases of HSCT and demonstrated the usefulness of proteomics and computational approaches as a revolutionary field in diagnostic methods. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-17 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/213591 10.1016/j.clinsp.2022.100134 |
url |
https://www.revistas.usp.br/clinics/article/view/213591 |
identifier_str_mv |
10.1016/j.clinsp.2022.100134 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/213591/195676 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Clinics info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Clinics |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 77 (2022); 100134 Clinics; v. 77 (2022); 100134 Clinics; Vol. 77 (2022); 100134 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222767027585024 |