Effect of a gel containing pilocarpine on vaginal atrophy in castrated rats
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/116025 |
Resumo: | OBJECTIVES: To evaluate the effect of Carbopol gel formulations containing pilocarpine on the morphology and morphometry of the vaginal epithelium of castrated rats. METHODS: Thirty-one female Wistar-Hannover rats were randomly divided into four groups: the control Groups I (n=7, rats in persistent estrus; positive controls) and II (n=7, castrated rats, negative controls) and the experimental Groups, III (n=8) and IV (n=9). Persistent estrus (Group I) was achieved with a subcutaneous injection of testosterone propionate on the second postnatal day. At 90 days postnatal, rats in Groups II, III and IV were castrated and treated vaginally for 14 days with Carbopol gel (vehicle alone) or Carbopol gel containing 5% and 15% pilocarpine, respectively. Next, all of the animals were euthanized and their vaginas were removed for histological evaluation. A non-parametric test with a weighted linear regression model was used for data analysis (p |
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Clinics |
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Effect of a gel containing pilocarpine on vaginal atrophy in castrated rats OBJECTIVES: To evaluate the effect of Carbopol gel formulations containing pilocarpine on the morphology and morphometry of the vaginal epithelium of castrated rats. METHODS: Thirty-one female Wistar-Hannover rats were randomly divided into four groups: the control Groups I (n=7, rats in persistent estrus; positive controls) and II (n=7, castrated rats, negative controls) and the experimental Groups, III (n=8) and IV (n=9). Persistent estrus (Group I) was achieved with a subcutaneous injection of testosterone propionate on the second postnatal day. At 90 days postnatal, rats in Groups II, III and IV were castrated and treated vaginally for 14 days with Carbopol gel (vehicle alone) or Carbopol gel containing 5% and 15% pilocarpine, respectively. Next, all of the animals were euthanized and their vaginas were removed for histological evaluation. A non-parametric test with a weighted linear regression model was used for data analysis (pHospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2016-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/11602510.6061/clinics/2016(05)09Clinics; Vol. 71 No. 5 (2016); 291-294Clinics; v. 71 n. 5 (2016); 291-294Clinics; Vol. 71 Núm. 5 (2016); 291-2941980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/116025/113689Copyright (c) 2016 Clinicsinfo:eu-repo/semantics/openAccessde Sousa-Lages, Cristina A.de Deus-Lages, Lívio P.de Sousa, Gabriela V.de Moura-Leal, Adinaide C.Conde Júnior, Airton MendesCosta-Silva, Danylo Rafhaelda Conceição Barros-Oliveira, MariaBorges, Carine SoaresEscórcio-Dourado, Carla SolangeSampaio, Fabiane A.Cunha-Nunes, Lívio C.da-Silva, Benedito B.2016-06-03T16:10:35Zoai:revistas.usp.br:article/116025Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2016-06-03T16:10:35Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Effect of a gel containing pilocarpine on vaginal atrophy in castrated rats |
title |
Effect of a gel containing pilocarpine on vaginal atrophy in castrated rats |
spellingShingle |
Effect of a gel containing pilocarpine on vaginal atrophy in castrated rats de Sousa-Lages, Cristina A. |
title_short |
Effect of a gel containing pilocarpine on vaginal atrophy in castrated rats |
title_full |
Effect of a gel containing pilocarpine on vaginal atrophy in castrated rats |
title_fullStr |
Effect of a gel containing pilocarpine on vaginal atrophy in castrated rats |
title_full_unstemmed |
Effect of a gel containing pilocarpine on vaginal atrophy in castrated rats |
title_sort |
Effect of a gel containing pilocarpine on vaginal atrophy in castrated rats |
author |
de Sousa-Lages, Cristina A. |
author_facet |
de Sousa-Lages, Cristina A. de Deus-Lages, Lívio P. de Sousa, Gabriela V. de Moura-Leal, Adinaide C. Conde Júnior, Airton Mendes Costa-Silva, Danylo Rafhael da Conceição Barros-Oliveira, Maria Borges, Carine Soares Escórcio-Dourado, Carla Solange Sampaio, Fabiane A. Cunha-Nunes, Lívio C. da-Silva, Benedito B. |
author_role |
author |
author2 |
de Deus-Lages, Lívio P. de Sousa, Gabriela V. de Moura-Leal, Adinaide C. Conde Júnior, Airton Mendes Costa-Silva, Danylo Rafhael da Conceição Barros-Oliveira, Maria Borges, Carine Soares Escórcio-Dourado, Carla Solange Sampaio, Fabiane A. Cunha-Nunes, Lívio C. da-Silva, Benedito B. |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
de Sousa-Lages, Cristina A. de Deus-Lages, Lívio P. de Sousa, Gabriela V. de Moura-Leal, Adinaide C. Conde Júnior, Airton Mendes Costa-Silva, Danylo Rafhael da Conceição Barros-Oliveira, Maria Borges, Carine Soares Escórcio-Dourado, Carla Solange Sampaio, Fabiane A. Cunha-Nunes, Lívio C. da-Silva, Benedito B. |
description |
OBJECTIVES: To evaluate the effect of Carbopol gel formulations containing pilocarpine on the morphology and morphometry of the vaginal epithelium of castrated rats. METHODS: Thirty-one female Wistar-Hannover rats were randomly divided into four groups: the control Groups I (n=7, rats in persistent estrus; positive controls) and II (n=7, castrated rats, negative controls) and the experimental Groups, III (n=8) and IV (n=9). Persistent estrus (Group I) was achieved with a subcutaneous injection of testosterone propionate on the second postnatal day. At 90 days postnatal, rats in Groups II, III and IV were castrated and treated vaginally for 14 days with Carbopol gel (vehicle alone) or Carbopol gel containing 5% and 15% pilocarpine, respectively. Next, all of the animals were euthanized and their vaginas were removed for histological evaluation. A non-parametric test with a weighted linear regression model was used for data analysis (p |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/116025 10.6061/clinics/2016(05)09 |
url |
https://www.revistas.usp.br/clinics/article/view/116025 |
identifier_str_mv |
10.6061/clinics/2016(05)09 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/116025/113689 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2016 Clinics info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2016 Clinics |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 71 No. 5 (2016); 291-294 Clinics; v. 71 n. 5 (2016); 291-294 Clinics; Vol. 71 Núm. 5 (2016); 291-294 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222762620420096 |