Gender differences in microcirculation: Observation using the hamster cheek pouch
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/77054 |
Resumo: | OBJECTIVES: Estrogen has been shown to play an important protective role in non-reproductive systems, such as the cardiovascular system. Our aim was to observe gender differences in vivo with regard to the increase in macromolecular permeability and leukocyte-endothelium interaction induced by ischemia/reperfusion as well as in microvascular reactivity to vasoactive substances using the hamster cheek pouch preparation. METHODS: Thirty-six male and 36 female hamsters, 21 weeks old, were selected for this study, and their cheek pouches were prepared for intravital microscopy. An increase in the macromolecular permeability of post-capillary venules was quantified as a leakage of intravenously injected fluorescein-labeled dextran, and the leukocyte-endothelium interaction was measured as the number of fluorescent rolling leukocytes or leukocytes adherent to the venular wall, labeled with rhodamin G, during reperfusion after 30 min of local ischemia. For microvascular reactivity, the mean internal diameter of arterioles was evaluated after the topical application of different concentrations of two vasoconstrictors, phenylephrine (α1-agonist) and endothelin-1, and two vasodilators, acetylcholine (endothelial-dependent) and sodium nitroprusside (endothelial-independent). RESULTS: The increase in macromolecular permeability induced by ischemia/reperfusion was significantly lower in females compared with males [19 (17-22) leaks/cm2 vs. 124 (123-128) leaks/cm2, respectively, p |
id |
USP-19_c94d6bef4e95eb75035af330096b906c |
---|---|
oai_identifier_str |
oai:revistas.usp.br:article/77054 |
network_acronym_str |
USP-19 |
network_name_str |
Clinics |
repository_id_str |
|
spelling |
Gender differences in microcirculation: Observation using the hamster cheek pouchOBJECTIVES: Estrogen has been shown to play an important protective role in non-reproductive systems, such as the cardiovascular system. Our aim was to observe gender differences in vivo with regard to the increase in macromolecular permeability and leukocyte-endothelium interaction induced by ischemia/reperfusion as well as in microvascular reactivity to vasoactive substances using the hamster cheek pouch preparation. METHODS: Thirty-six male and 36 female hamsters, 21 weeks old, were selected for this study, and their cheek pouches were prepared for intravital microscopy. An increase in the macromolecular permeability of post-capillary venules was quantified as a leakage of intravenously injected fluorescein-labeled dextran, and the leukocyte-endothelium interaction was measured as the number of fluorescent rolling leukocytes or leukocytes adherent to the venular wall, labeled with rhodamin G, during reperfusion after 30 min of local ischemia. For microvascular reactivity, the mean internal diameter of arterioles was evaluated after the topical application of different concentrations of two vasoconstrictors, phenylephrine (α1-agonist) and endothelin-1, and two vasodilators, acetylcholine (endothelial-dependent) and sodium nitroprusside (endothelial-independent). RESULTS: The increase in macromolecular permeability induced by ischemia/reperfusion was significantly lower in females compared with males [19 (17-22) leaks/cm2 vs. 124 (123-128) leaks/cm2, respectively, pHospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2013-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/7705410.1590/clin.v68i12.77054Clinics; Vol. 68 No. 12 (2013); 1537-1542Clinics; v. 68 n. 12 (2013); 1537-1542Clinics; Vol. 68 Núm. 12 (2013); 1537-15421980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/77054/80919Panazzolo, Diogo Guarnierida Silva, Lucia Henriques Alvesde Almeida Cyrino, Fatima Zely GarciaSicuro, Fernando LencastreKraemer-Aguiar, Luiz GuilhermeBouskela, Elieteinfo:eu-repo/semantics/openAccess2014-03-24T18:38:58Zoai:revistas.usp.br:article/77054Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2014-03-24T18:38:58Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Gender differences in microcirculation: Observation using the hamster cheek pouch |
title |
Gender differences in microcirculation: Observation using the hamster cheek pouch |
spellingShingle |
Gender differences in microcirculation: Observation using the hamster cheek pouch Panazzolo, Diogo Guarnieri |
title_short |
Gender differences in microcirculation: Observation using the hamster cheek pouch |
title_full |
Gender differences in microcirculation: Observation using the hamster cheek pouch |
title_fullStr |
Gender differences in microcirculation: Observation using the hamster cheek pouch |
title_full_unstemmed |
Gender differences in microcirculation: Observation using the hamster cheek pouch |
title_sort |
Gender differences in microcirculation: Observation using the hamster cheek pouch |
author |
Panazzolo, Diogo Guarnieri |
author_facet |
Panazzolo, Diogo Guarnieri da Silva, Lucia Henriques Alves de Almeida Cyrino, Fatima Zely Garcia Sicuro, Fernando Lencastre Kraemer-Aguiar, Luiz Guilherme Bouskela, Eliete |
author_role |
author |
author2 |
da Silva, Lucia Henriques Alves de Almeida Cyrino, Fatima Zely Garcia Sicuro, Fernando Lencastre Kraemer-Aguiar, Luiz Guilherme Bouskela, Eliete |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Panazzolo, Diogo Guarnieri da Silva, Lucia Henriques Alves de Almeida Cyrino, Fatima Zely Garcia Sicuro, Fernando Lencastre Kraemer-Aguiar, Luiz Guilherme Bouskela, Eliete |
description |
OBJECTIVES: Estrogen has been shown to play an important protective role in non-reproductive systems, such as the cardiovascular system. Our aim was to observe gender differences in vivo with regard to the increase in macromolecular permeability and leukocyte-endothelium interaction induced by ischemia/reperfusion as well as in microvascular reactivity to vasoactive substances using the hamster cheek pouch preparation. METHODS: Thirty-six male and 36 female hamsters, 21 weeks old, were selected for this study, and their cheek pouches were prepared for intravital microscopy. An increase in the macromolecular permeability of post-capillary venules was quantified as a leakage of intravenously injected fluorescein-labeled dextran, and the leukocyte-endothelium interaction was measured as the number of fluorescent rolling leukocytes or leukocytes adherent to the venular wall, labeled with rhodamin G, during reperfusion after 30 min of local ischemia. For microvascular reactivity, the mean internal diameter of arterioles was evaluated after the topical application of different concentrations of two vasoconstrictors, phenylephrine (α1-agonist) and endothelin-1, and two vasodilators, acetylcholine (endothelial-dependent) and sodium nitroprusside (endothelial-independent). RESULTS: The increase in macromolecular permeability induced by ischemia/reperfusion was significantly lower in females compared with males [19 (17-22) leaks/cm2 vs. 124 (123-128) leaks/cm2, respectively, p |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-31 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/77054 10.1590/clin.v68i12.77054 |
url |
https://www.revistas.usp.br/clinics/article/view/77054 |
identifier_str_mv |
10.1590/clin.v68i12.77054 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/77054/80919 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 68 No. 12 (2013); 1537-1542 Clinics; v. 68 n. 12 (2013); 1537-1542 Clinics; Vol. 68 Núm. 12 (2013); 1537-1542 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222760806383616 |