Behaviour of the T. cruzi blood forms obtained from mice used as biological filters in culture and triatomine
Autor(a) principal: | |
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Data de Publicação: | 1986 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista de Saúde Pública |
Texto Completo: | https://www.revistas.usp.br/rsp/article/view/23342 |
Resumo: | The study of the behaviour of T. cruzi strains in animals as in human beings, runs into great difficulties when the attempt is made to relate the morphology of this parasite to its biological behaviour. The thin forms are able to penetrate vertebrate cells faster than the broad forms. So, faced with the biological complexity of the T. cruzi strains and substrains it was decided to investigate the behaviour of its blood forms obtained from normal mice as biological filters. These mice were endovenously inoculated with blood trypomastigotes from derivated RCL of the T. cruzi RC strain. Forty-eight hours later, the animals went through xenodiagnosis and heart punch in order to sow the blood in Warren's medium. The trypomastigotes became round (spheromastigotes and/or amastigotes) in the stomach of the triatomines and cultures at 28°C. The behaviour of the broad forms has been frequently observed and leads to the conclusion that the biology of the T. cruzi is not only related to the strain, but, sometimes, to the parasite population. |
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Behaviour of the T. cruzi blood forms obtained from mice used as biological filters in culture and triatomine Comportamento de formas sangüícolas do Trypanosoma cruzi obtidas de camundongos utilizados como filtros biológicos, em cultura e em triatomíneo Trypanosoma cruziCamundongos^i1^sinfecção experimenTriatomíneos^i1^sparasitoloMacrófagos^i1^sparasitoloRelações hospedeiro-parasitaTrypanosoma cruziMice^i2^sexperimental infectiTriatomidae^i2^sparasitolMachophages^i2^sparasitolHost-parasite relations The study of the behaviour of T. cruzi strains in animals as in human beings, runs into great difficulties when the attempt is made to relate the morphology of this parasite to its biological behaviour. The thin forms are able to penetrate vertebrate cells faster than the broad forms. So, faced with the biological complexity of the T. cruzi strains and substrains it was decided to investigate the behaviour of its blood forms obtained from normal mice as biological filters. These mice were endovenously inoculated with blood trypomastigotes from derivated RCL of the T. cruzi RC strain. Forty-eight hours later, the animals went through xenodiagnosis and heart punch in order to sow the blood in Warren's medium. The trypomastigotes became round (spheromastigotes and/or amastigotes) in the stomach of the triatomines and cultures at 28°C. The behaviour of the broad forms has been frequently observed and leads to the conclusion that the biology of the T. cruzi is not only related to the strain, but, sometimes, to the parasite population. Camundongos normais, utilizados como filtros biológicos, foram inoculados endovenosamente, com tripomastigotas sangüícolas da derivada RCL da cepa RC do T. cruzi. Decorridas 48 horas, os animais foram submetidos ao xenodiagnóstico e à punção cardíaca para semeadura do sangue em meio de Warren. No estômago dos triatomíneos e em cultura a 28°C, os tripomastigotas diferenciaram-se em formas arredondadas (esferomastigotas e/ou amastigotas). Esse comportamento das formas sangüícolas largas tem sido observado com freqüência e nos leva a inferir que a biologia do T. cruzi não estaria apenas relacionada com a cepa, mas, eventualmente, com populações do parasita. Universidade de São Paulo. Faculdade de Saúde Pública1986-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rsp/article/view/2334210.1590/S0034-89101986000200003Revista de Saúde Pública; Vol. 20 No. 2 (1986); 129-132 Revista de Saúde Pública; Vol. 20 Núm. 2 (1986); 129-132 Revista de Saúde Pública; v. 20 n. 2 (1986); 129-132 1518-87870034-8910reponame:Revista de Saúde Públicainstname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/rsp/article/view/23342/25371Copyright (c) 2017 Revista de Saúde Públicainfo:eu-repo/semantics/openAccessRibeiro, Rosa DominguesBelda Neto, Francisco MiguelPrado Junior, José Clóvis doAlbuquerque, Sérgio de2012-05-28T16:22:46Zoai:revistas.usp.br:article/23342Revistahttps://www.revistas.usp.br/rsp/indexONGhttps://www.revistas.usp.br/rsp/oairevsp@org.usp.br||revsp1@usp.br1518-87870034-8910opendoar:2012-05-28T16:22:46Revista de Saúde Pública - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Behaviour of the T. cruzi blood forms obtained from mice used as biological filters in culture and triatomine Comportamento de formas sangüícolas do Trypanosoma cruzi obtidas de camundongos utilizados como filtros biológicos, em cultura e em triatomíneo |
title |
Behaviour of the T. cruzi blood forms obtained from mice used as biological filters in culture and triatomine |
spellingShingle |
Behaviour of the T. cruzi blood forms obtained from mice used as biological filters in culture and triatomine Ribeiro, Rosa Domingues Trypanosoma cruzi Camundongos^i1^sinfecção experimen Triatomíneos^i1^sparasitolo Macrófagos^i1^sparasitolo Relações hospedeiro-parasita Trypanosoma cruzi Mice^i2^sexperimental infecti Triatomidae^i2^sparasitol Machophages^i2^sparasitol Host-parasite relations |
title_short |
Behaviour of the T. cruzi blood forms obtained from mice used as biological filters in culture and triatomine |
title_full |
Behaviour of the T. cruzi blood forms obtained from mice used as biological filters in culture and triatomine |
title_fullStr |
Behaviour of the T. cruzi blood forms obtained from mice used as biological filters in culture and triatomine |
title_full_unstemmed |
Behaviour of the T. cruzi blood forms obtained from mice used as biological filters in culture and triatomine |
title_sort |
Behaviour of the T. cruzi blood forms obtained from mice used as biological filters in culture and triatomine |
author |
Ribeiro, Rosa Domingues |
author_facet |
Ribeiro, Rosa Domingues Belda Neto, Francisco Miguel Prado Junior, José Clóvis do Albuquerque, Sérgio de |
author_role |
author |
author2 |
Belda Neto, Francisco Miguel Prado Junior, José Clóvis do Albuquerque, Sérgio de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Ribeiro, Rosa Domingues Belda Neto, Francisco Miguel Prado Junior, José Clóvis do Albuquerque, Sérgio de |
dc.subject.por.fl_str_mv |
Trypanosoma cruzi Camundongos^i1^sinfecção experimen Triatomíneos^i1^sparasitolo Macrófagos^i1^sparasitolo Relações hospedeiro-parasita Trypanosoma cruzi Mice^i2^sexperimental infecti Triatomidae^i2^sparasitol Machophages^i2^sparasitol Host-parasite relations |
topic |
Trypanosoma cruzi Camundongos^i1^sinfecção experimen Triatomíneos^i1^sparasitolo Macrófagos^i1^sparasitolo Relações hospedeiro-parasita Trypanosoma cruzi Mice^i2^sexperimental infecti Triatomidae^i2^sparasitol Machophages^i2^sparasitol Host-parasite relations |
description |
The study of the behaviour of T. cruzi strains in animals as in human beings, runs into great difficulties when the attempt is made to relate the morphology of this parasite to its biological behaviour. The thin forms are able to penetrate vertebrate cells faster than the broad forms. So, faced with the biological complexity of the T. cruzi strains and substrains it was decided to investigate the behaviour of its blood forms obtained from normal mice as biological filters. These mice were endovenously inoculated with blood trypomastigotes from derivated RCL of the T. cruzi RC strain. Forty-eight hours later, the animals went through xenodiagnosis and heart punch in order to sow the blood in Warren's medium. The trypomastigotes became round (spheromastigotes and/or amastigotes) in the stomach of the triatomines and cultures at 28°C. The behaviour of the broad forms has been frequently observed and leads to the conclusion that the biology of the T. cruzi is not only related to the strain, but, sometimes, to the parasite population. |
publishDate |
1986 |
dc.date.none.fl_str_mv |
1986-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rsp/article/view/23342 10.1590/S0034-89101986000200003 |
url |
https://www.revistas.usp.br/rsp/article/view/23342 |
identifier_str_mv |
10.1590/S0034-89101986000200003 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rsp/article/view/23342/25371 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Revista de Saúde Pública info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Revista de Saúde Pública |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Saúde Pública |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Saúde Pública |
dc.source.none.fl_str_mv |
Revista de Saúde Pública; Vol. 20 No. 2 (1986); 129-132 Revista de Saúde Pública; Vol. 20 Núm. 2 (1986); 129-132 Revista de Saúde Pública; v. 20 n. 2 (1986); 129-132 1518-8787 0034-8910 reponame:Revista de Saúde Pública instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Revista de Saúde Pública |
collection |
Revista de Saúde Pública |
repository.name.fl_str_mv |
Revista de Saúde Pública - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
revsp@org.usp.br||revsp1@usp.br |
_version_ |
1800221773177815040 |