Development and Characterization of a Hydrogel Containing Silver Sulfadiazine for Antimicrobial Topical Applications: Part II: Stability, Cytotoxicity and Silver Release Patterns
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/204331 |
Resumo: | Hydrogels are interesting for use in the treatment of topical wounds due to their virtually zero toxicity, and capacity for extended release of pharmaceuticals. Silver sulfadiazine (SSDZ) is the drug of choice in the treatment of skin burns. The aim of the study was to determine cytotoxicity, antimicrobial activity and stability of a PVA hydrogel with integrated silver sulfadiazine. SSDZ-hydrogels were prepared using 10% (w/w) PVA (either 89% or 99% hydrolyzed) and 1% (w/w) silver sulfadiazine. Cellular viability was assessed via MTS assays, antimicrobial activity via disk-diffusion and accelerated stability tests were carried out with analysis at 0, 30, 60, 90 and 180 days of storage at 40 ± 2 °C and a relative humidity of 75 ± 5%. The parameters evaluated included organoleptic characteristics, moisture, swelling ability, mechanical strength, FTIR, XRD, TGA and DSC, and silver release patterns via XRD and potentiometry. Cell viability tests indicated some cytotoxicity, although within acceptable levels. After 90 days of storage, SSDZ hydrogel samples exhibited a brown coloration, probably due to the formation of Ag or Ag2O nanoparticles. The SSDZ-loaded hydrogels suffered visual and physical changes; however, these changes did not compromise its use as occlusive wound dressings or its antimicrobial properties. |
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oai:revistas.usp.br:article/204331 |
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Brazilian Journal of Pharmaceutical Sciences |
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Development and Characterization of a Hydrogel Containing Silver Sulfadiazine for Antimicrobial Topical Applications: Part II: Stability, Cytotoxicity and Silver Release PatternsHydrogelSilver sulfadiazineAntimicrobial topical applicationsHydrogels are interesting for use in the treatment of topical wounds due to their virtually zero toxicity, and capacity for extended release of pharmaceuticals. Silver sulfadiazine (SSDZ) is the drug of choice in the treatment of skin burns. The aim of the study was to determine cytotoxicity, antimicrobial activity and stability of a PVA hydrogel with integrated silver sulfadiazine. SSDZ-hydrogels were prepared using 10% (w/w) PVA (either 89% or 99% hydrolyzed) and 1% (w/w) silver sulfadiazine. Cellular viability was assessed via MTS assays, antimicrobial activity via disk-diffusion and accelerated stability tests were carried out with analysis at 0, 30, 60, 90 and 180 days of storage at 40 ± 2 °C and a relative humidity of 75 ± 5%. The parameters evaluated included organoleptic characteristics, moisture, swelling ability, mechanical strength, FTIR, XRD, TGA and DSC, and silver release patterns via XRD and potentiometry. Cell viability tests indicated some cytotoxicity, although within acceptable levels. After 90 days of storage, SSDZ hydrogel samples exhibited a brown coloration, probably due to the formation of Ag or Ag2O nanoparticles. The SSDZ-loaded hydrogels suffered visual and physical changes; however, these changes did not compromise its use as occlusive wound dressings or its antimicrobial properties.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2022-11-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/20433110.1590/s2175-97902022e18688Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)Brazilian Journal of Pharmaceutical Sciences; v. 58 (2022)Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)2175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/204331/194941Copyright (c) 2022 Brazilian Journal of Pharmaceutical Scienceshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessVaz, Lillian VieiraBalcão, Victor ManuelOliveira Junior, José MartinsTubino, MatthieuJozala, AngelaYoshida, Valquiria Miwa HanaiVila, Marta Maria Duarte Carvalho2023-08-18T19:56:31Zoai:revistas.usp.br:article/204331Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2023-08-18T19:56:31Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Development and Characterization of a Hydrogel Containing Silver Sulfadiazine for Antimicrobial Topical Applications: Part II: Stability, Cytotoxicity and Silver Release Patterns |
title |
Development and Characterization of a Hydrogel Containing Silver Sulfadiazine for Antimicrobial Topical Applications: Part II: Stability, Cytotoxicity and Silver Release Patterns |
spellingShingle |
Development and Characterization of a Hydrogel Containing Silver Sulfadiazine for Antimicrobial Topical Applications: Part II: Stability, Cytotoxicity and Silver Release Patterns Vaz, Lillian Vieira Hydrogel Silver sulfadiazine Antimicrobial topical applications |
title_short |
Development and Characterization of a Hydrogel Containing Silver Sulfadiazine for Antimicrobial Topical Applications: Part II: Stability, Cytotoxicity and Silver Release Patterns |
title_full |
Development and Characterization of a Hydrogel Containing Silver Sulfadiazine for Antimicrobial Topical Applications: Part II: Stability, Cytotoxicity and Silver Release Patterns |
title_fullStr |
Development and Characterization of a Hydrogel Containing Silver Sulfadiazine for Antimicrobial Topical Applications: Part II: Stability, Cytotoxicity and Silver Release Patterns |
title_full_unstemmed |
Development and Characterization of a Hydrogel Containing Silver Sulfadiazine for Antimicrobial Topical Applications: Part II: Stability, Cytotoxicity and Silver Release Patterns |
title_sort |
Development and Characterization of a Hydrogel Containing Silver Sulfadiazine for Antimicrobial Topical Applications: Part II: Stability, Cytotoxicity and Silver Release Patterns |
author |
Vaz, Lillian Vieira |
author_facet |
Vaz, Lillian Vieira Balcão, Victor Manuel Oliveira Junior, José Martins Tubino, Matthieu Jozala, Angela Yoshida, Valquiria Miwa Hanai Vila, Marta Maria Duarte Carvalho |
author_role |
author |
author2 |
Balcão, Victor Manuel Oliveira Junior, José Martins Tubino, Matthieu Jozala, Angela Yoshida, Valquiria Miwa Hanai Vila, Marta Maria Duarte Carvalho |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Vaz, Lillian Vieira Balcão, Victor Manuel Oliveira Junior, José Martins Tubino, Matthieu Jozala, Angela Yoshida, Valquiria Miwa Hanai Vila, Marta Maria Duarte Carvalho |
dc.subject.por.fl_str_mv |
Hydrogel Silver sulfadiazine Antimicrobial topical applications |
topic |
Hydrogel Silver sulfadiazine Antimicrobial topical applications |
description |
Hydrogels are interesting for use in the treatment of topical wounds due to their virtually zero toxicity, and capacity for extended release of pharmaceuticals. Silver sulfadiazine (SSDZ) is the drug of choice in the treatment of skin burns. The aim of the study was to determine cytotoxicity, antimicrobial activity and stability of a PVA hydrogel with integrated silver sulfadiazine. SSDZ-hydrogels were prepared using 10% (w/w) PVA (either 89% or 99% hydrolyzed) and 1% (w/w) silver sulfadiazine. Cellular viability was assessed via MTS assays, antimicrobial activity via disk-diffusion and accelerated stability tests were carried out with analysis at 0, 30, 60, 90 and 180 days of storage at 40 ± 2 °C and a relative humidity of 75 ± 5%. The parameters evaluated included organoleptic characteristics, moisture, swelling ability, mechanical strength, FTIR, XRD, TGA and DSC, and silver release patterns via XRD and potentiometry. Cell viability tests indicated some cytotoxicity, although within acceptable levels. After 90 days of storage, SSDZ hydrogel samples exhibited a brown coloration, probably due to the formation of Ag or Ag2O nanoparticles. The SSDZ-loaded hydrogels suffered visual and physical changes; however, these changes did not compromise its use as occlusive wound dressings or its antimicrobial properties. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/204331 10.1590/s2175-97902022e18688 |
url |
https://www.revistas.usp.br/bjps/article/view/204331 |
identifier_str_mv |
10.1590/s2175-97902022e18688 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/204331/194941 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022) Brazilian Journal of Pharmaceutical Sciences; v. 58 (2022) Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022) 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1800222916047011840 |