Monocyte Activation Test (MAT) as a possibility of replacement for the rabbit pyrogen test in hyperimmune sera

Detalhes bibliográficos
Autor(a) principal: Utescher, Carla Lilian de Agostini
Data de Publicação: 2018
Outros Autores: Buosi, Klariane Laís, Botosso, Viviane Fungaro, Quintilio, Wagner
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Pharmaceutical Sciences
Texto Completo: https://www.revistas.usp.br/bjps/article/view/153803
Resumo: The use of a commercial kit for the monocyte-activation test (MAT) was evaluated for assessing pyrogenic contamination of hyperimmune sera . Three batches of sera, two pyrogen free and one pyrogenic, were tested. Endotoxin spike recover indicated that sample dilutions from 1/2 to 1/10 are suitable. Kit transport and storage conditions were also evaluated, proving that an adequate cold chain must be assured to achieve good results. Furthermore, the commercial MAT kit seemed suitable to replace the rabbit pyrogen test (RPT) for pyrogen testing of hyperimmune sera, although further tests are needed to a full validation.
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spelling Monocyte Activation Test (MAT) as a possibility of replacement for the rabbit pyrogen test in hyperimmune seraMonocytes Activation TestPyrogenHyperimmune serumPyrodetect kitCryobloodThe use of a commercial kit for the monocyte-activation test (MAT) was evaluated for assessing pyrogenic contamination of hyperimmune sera . Three batches of sera, two pyrogen free and one pyrogenic, were tested. Endotoxin spike recover indicated that sample dilutions from 1/2 to 1/10 are suitable. Kit transport and storage conditions were also evaluated, proving that an adequate cold chain must be assured to achieve good results. Furthermore, the commercial MAT kit seemed suitable to replace the rabbit pyrogen test (RPT) for pyrogen testing of hyperimmune sera, although further tests are needed to a full validation.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2018-07-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/15380310.1590/s2175-97902018000217530Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. 2 (2018); e17530Brazilian Journal of Pharmaceutical Sciences; v. 54 n. 2 (2018); e17530Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. 2 (2018); e175302175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/153803/150186Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)info:eu-repo/semantics/openAccessUtescher, Carla Lilian de AgostiniBuosi, Klariane LaísBotosso, Viviane FungaroQuintilio, Wagner2019-03-17T13:56:55Zoai:revistas.usp.br:article/153803Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2019-03-17T13:56:55Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Monocyte Activation Test (MAT) as a possibility of replacement for the rabbit pyrogen test in hyperimmune sera
title Monocyte Activation Test (MAT) as a possibility of replacement for the rabbit pyrogen test in hyperimmune sera
spellingShingle Monocyte Activation Test (MAT) as a possibility of replacement for the rabbit pyrogen test in hyperimmune sera
Utescher, Carla Lilian de Agostini
Monocytes Activation Test
Pyrogen
Hyperimmune serum
Pyrodetect kit
Cryoblood
title_short Monocyte Activation Test (MAT) as a possibility of replacement for the rabbit pyrogen test in hyperimmune sera
title_full Monocyte Activation Test (MAT) as a possibility of replacement for the rabbit pyrogen test in hyperimmune sera
title_fullStr Monocyte Activation Test (MAT) as a possibility of replacement for the rabbit pyrogen test in hyperimmune sera
title_full_unstemmed Monocyte Activation Test (MAT) as a possibility of replacement for the rabbit pyrogen test in hyperimmune sera
title_sort Monocyte Activation Test (MAT) as a possibility of replacement for the rabbit pyrogen test in hyperimmune sera
author Utescher, Carla Lilian de Agostini
author_facet Utescher, Carla Lilian de Agostini
Buosi, Klariane Laís
Botosso, Viviane Fungaro
Quintilio, Wagner
author_role author
author2 Buosi, Klariane Laís
Botosso, Viviane Fungaro
Quintilio, Wagner
author2_role author
author
author
dc.contributor.author.fl_str_mv Utescher, Carla Lilian de Agostini
Buosi, Klariane Laís
Botosso, Viviane Fungaro
Quintilio, Wagner
dc.subject.por.fl_str_mv Monocytes Activation Test
Pyrogen
Hyperimmune serum
Pyrodetect kit
Cryoblood
topic Monocytes Activation Test
Pyrogen
Hyperimmune serum
Pyrodetect kit
Cryoblood
description The use of a commercial kit for the monocyte-activation test (MAT) was evaluated for assessing pyrogenic contamination of hyperimmune sera . Three batches of sera, two pyrogen free and one pyrogenic, were tested. Endotoxin spike recover indicated that sample dilutions from 1/2 to 1/10 are suitable. Kit transport and storage conditions were also evaluated, proving that an adequate cold chain must be assured to achieve good results. Furthermore, the commercial MAT kit seemed suitable to replace the rabbit pyrogen test (RPT) for pyrogen testing of hyperimmune sera, although further tests are needed to a full validation.
publishDate 2018
dc.date.none.fl_str_mv 2018-07-26
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjps/article/view/153803
10.1590/s2175-97902018000217530
url https://www.revistas.usp.br/bjps/article/view/153803
identifier_str_mv 10.1590/s2175-97902018000217530
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjps/article/view/153803/150186
dc.rights.driver.fl_str_mv Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
dc.source.none.fl_str_mv Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. 2 (2018); e17530
Brazilian Journal of Pharmaceutical Sciences; v. 54 n. 2 (2018); e17530
Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. 2 (2018); e17530
2175-9790
1984-8250
reponame:Brazilian Journal of Pharmaceutical Sciences
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Pharmaceutical Sciences
collection Brazilian Journal of Pharmaceutical Sciences
repository.name.fl_str_mv Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)
repository.mail.fl_str_mv bjps@usp.br||elizabeth.igne@gmail.com
_version_ 1800222913735950336

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