Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/164494 |
Resumo: | To study the effect of small interfering RNA targeting metastasis-associated lung adenocarcinoma transcript1 (si-MALAT1) combining with curcumin on the invasion and migration abilities of human colon cancer SW480 cells, and to explore the involved molecular mechanism. The recombinant lentiviral vector expressing si-MALAT1 was constructed, and its titer was determined by gradient dilution method. The colon cancer SW480 cells with stable expression of si-MALAT1 was established, followed by treatment with curcumin at different concentrations. The effect of curcumin or si-MALAT1 alone and the combination of the two on the cell activity was detected by MTT assay. The cell invasion and migration abilities were detected by transwell and scratch-wound assay. The relative expression level of MALAT1 was detected by RT-qPCR. The protein expression was determined by Western blot analysis. The IC50 of curcumin alone was 77.69 µmol/L, which was 51.17 mol/Lwhen combined with curcumin and random sequence. The IC50 of curcumin was 30.02 µmol/L when combined with si-MALAT1. The increased susceptibility multiples was 2.58. The wound healing rates were 30.9% and 67.5% after treatment with si-MALAT1 combined with curcumin for 24 hrs and 48 hrs, respectively. The numbers of invasion cells were 200±12, 162±13, 66±8, 53±4 and 16±3 after treatment with si-MALAT1 combined with curcumin for 48 hrs. The relative expression level of lncRNA-MALAT1 in the curcumin group was 68%, and the relative expression level of lncRNA-MALAT1 in si-MALAT1group was 56%, and that for the combination treatment group was about 21%. The protein expression levels of β- catenin, c-myc and cyclinD1 were significantly down-regulated upon treatment with certain concentration of si-MALAT1 alone or combined with curcumin.si-MALAT1 could significantly inhibit the invasion and migration of SW480 cells by enhancing the sensitivity of SW480 cells to curcumin. The mechanism involved mignt be related to the down-regulation of β-catenin, c-myc and cyclinD1 proteins. |
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oai:revistas.usp.br:article/164494 |
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Brazilian Journal of Pharmaceutical Sciences |
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Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cellsCurcumin/ pharmacologyLncRNA-MALAT1RNA, Small Interfering/ drug effectsColon cancerInvasionMigrationColonic Neoplasms/ prevention & controlColonic Neoplasms/ drug therapyNeoplasm Invasiveness/ prevention & controlCell Migration Inhibition/ drug effectsTo study the effect of small interfering RNA targeting metastasis-associated lung adenocarcinoma transcript1 (si-MALAT1) combining with curcumin on the invasion and migration abilities of human colon cancer SW480 cells, and to explore the involved molecular mechanism. The recombinant lentiviral vector expressing si-MALAT1 was constructed, and its titer was determined by gradient dilution method. The colon cancer SW480 cells with stable expression of si-MALAT1 was established, followed by treatment with curcumin at different concentrations. The effect of curcumin or si-MALAT1 alone and the combination of the two on the cell activity was detected by MTT assay. The cell invasion and migration abilities were detected by transwell and scratch-wound assay. The relative expression level of MALAT1 was detected by RT-qPCR. The protein expression was determined by Western blot analysis. The IC50 of curcumin alone was 77.69 µmol/L, which was 51.17 mol/Lwhen combined with curcumin and random sequence. The IC50 of curcumin was 30.02 µmol/L when combined with si-MALAT1. The increased susceptibility multiples was 2.58. The wound healing rates were 30.9% and 67.5% after treatment with si-MALAT1 combined with curcumin for 24 hrs and 48 hrs, respectively. The numbers of invasion cells were 200±12, 162±13, 66±8, 53±4 and 16±3 after treatment with si-MALAT1 combined with curcumin for 48 hrs. The relative expression level of lncRNA-MALAT1 in the curcumin group was 68%, and the relative expression level of lncRNA-MALAT1 in si-MALAT1group was 56%, and that for the combination treatment group was about 21%. The protein expression levels of β- catenin, c-myc and cyclinD1 were significantly down-regulated upon treatment with certain concentration of si-MALAT1 alone or combined with curcumin.si-MALAT1 could significantly inhibit the invasion and migration of SW480 cells by enhancing the sensitivity of SW480 cells to curcumin. The mechanism involved mignt be related to the down-regulation of β-catenin, c-myc and cyclinD1 proteins.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2019-11-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/16449410.1590/s2175-97902019000118276Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e18276Brazilian Journal of Pharmaceutical Sciences; v. 55 (2019); e18276Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e182762175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/164494/157738Copyright (c) 2019 Brazilian Journal of Pharmaceutical Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessWei, DaiYun, LI ShiDejun, XiaoCong, LiuHe, Jin-HuaYan, Lin2021-01-11T17:43:32Zoai:revistas.usp.br:article/164494Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2021-01-11T17:43:32Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells |
title |
Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells |
spellingShingle |
Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells Wei, Dai Curcumin/ pharmacology LncRNA-MALAT1 RNA, Small Interfering/ drug effects Colon cancer Invasion Migration Colonic Neoplasms/ prevention & control Colonic Neoplasms/ drug therapy Neoplasm Invasiveness/ prevention & control Cell Migration Inhibition/ drug effects |
title_short |
Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells |
title_full |
Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells |
title_fullStr |
Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells |
title_full_unstemmed |
Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells |
title_sort |
Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells |
author |
Wei, Dai |
author_facet |
Wei, Dai Yun, LI Shi Dejun, Xiao Cong, Liu He, Jin-Hua Yan, Lin |
author_role |
author |
author2 |
Yun, LI Shi Dejun, Xiao Cong, Liu He, Jin-Hua Yan, Lin |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Wei, Dai Yun, LI Shi Dejun, Xiao Cong, Liu He, Jin-Hua Yan, Lin |
dc.subject.por.fl_str_mv |
Curcumin/ pharmacology LncRNA-MALAT1 RNA, Small Interfering/ drug effects Colon cancer Invasion Migration Colonic Neoplasms/ prevention & control Colonic Neoplasms/ drug therapy Neoplasm Invasiveness/ prevention & control Cell Migration Inhibition/ drug effects |
topic |
Curcumin/ pharmacology LncRNA-MALAT1 RNA, Small Interfering/ drug effects Colon cancer Invasion Migration Colonic Neoplasms/ prevention & control Colonic Neoplasms/ drug therapy Neoplasm Invasiveness/ prevention & control Cell Migration Inhibition/ drug effects |
description |
To study the effect of small interfering RNA targeting metastasis-associated lung adenocarcinoma transcript1 (si-MALAT1) combining with curcumin on the invasion and migration abilities of human colon cancer SW480 cells, and to explore the involved molecular mechanism. The recombinant lentiviral vector expressing si-MALAT1 was constructed, and its titer was determined by gradient dilution method. The colon cancer SW480 cells with stable expression of si-MALAT1 was established, followed by treatment with curcumin at different concentrations. The effect of curcumin or si-MALAT1 alone and the combination of the two on the cell activity was detected by MTT assay. The cell invasion and migration abilities were detected by transwell and scratch-wound assay. The relative expression level of MALAT1 was detected by RT-qPCR. The protein expression was determined by Western blot analysis. The IC50 of curcumin alone was 77.69 µmol/L, which was 51.17 mol/Lwhen combined with curcumin and random sequence. The IC50 of curcumin was 30.02 µmol/L when combined with si-MALAT1. The increased susceptibility multiples was 2.58. The wound healing rates were 30.9% and 67.5% after treatment with si-MALAT1 combined with curcumin for 24 hrs and 48 hrs, respectively. The numbers of invasion cells were 200±12, 162±13, 66±8, 53±4 and 16±3 after treatment with si-MALAT1 combined with curcumin for 48 hrs. The relative expression level of lncRNA-MALAT1 in the curcumin group was 68%, and the relative expression level of lncRNA-MALAT1 in si-MALAT1group was 56%, and that for the combination treatment group was about 21%. The protein expression levels of β- catenin, c-myc and cyclinD1 were significantly down-regulated upon treatment with certain concentration of si-MALAT1 alone or combined with curcumin.si-MALAT1 could significantly inhibit the invasion and migration of SW480 cells by enhancing the sensitivity of SW480 cells to curcumin. The mechanism involved mignt be related to the down-regulation of β-catenin, c-myc and cyclinD1 proteins. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/164494 10.1590/s2175-97902019000118276 |
url |
https://www.revistas.usp.br/bjps/article/view/164494 |
identifier_str_mv |
10.1590/s2175-97902019000118276 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/164494/157738 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Brazilian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Brazilian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e18276 Brazilian Journal of Pharmaceutical Sciences; v. 55 (2019); e18276 Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e18276 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1800222914434301952 |