Mycobacteria in Minas cheese commercialized in open fairs in São Paulo, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/e146525 |
Resumo: | Mycobacterium bovis is the causative agent of bovine tuberculosis, a disease that affects dairy herds throughout the Brazilian territory, constituting a neglected zoonosis transmitted by raw milk and its derivatives. In this study, we evaluated the presence of M. bovis and other mycobacteria in Minas cheese obtained from open fairs in the city of São Paulo between 2012 and 2013. Samples (n = 133) were decontaminated using hexa-cetylpyridinium chloride and seeded on Stonebrink–Leslie medium. The isolates were submitted to molecular identification by TB Multiplex PCR targeting the 16S rRNA gene and amplicon nucleotide sequencing. From 16 cheese samples (12%), we obtained 26 putative colonies of Mycobacterium spp., none of which belonged to any of the Mycobacterium tuberculosis, Mycobacterium avium, or Mycobacterium intracellulare complexes. Phylogenetic analysis showed that sample sequences were grouped in a clade that includes only non-tuberculous mycobacteria with proximity to sequences obtained from Mycobacterium novocastrense (3 sequences), Mycobacterium holsaticum (1 sequence), andMycobacterium elephantis (2 sequences). Although no epidemiological evidence was found regarding the importance of oral transmission of mycobacteria in healthy people, their importance in the immunosuppressed population remains uncertain. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Mycobacteria in Minas cheese commercialized in open fairs in São Paulo, BrazilMycobacteria em queijos tipo Minas comercializados em feiras-livres de São Paulo, BrasilMycobacteriaNon-tuberculous mycobacteriaMycobacterium bovisMinas cheeseFree-trade fairMycobacteriaMicobactérias não tuberculosasMycobacterium bovisQueijo tipo minasFeiras-livresMycobacterium bovis is the causative agent of bovine tuberculosis, a disease that affects dairy herds throughout the Brazilian territory, constituting a neglected zoonosis transmitted by raw milk and its derivatives. In this study, we evaluated the presence of M. bovis and other mycobacteria in Minas cheese obtained from open fairs in the city of São Paulo between 2012 and 2013. Samples (n = 133) were decontaminated using hexa-cetylpyridinium chloride and seeded on Stonebrink–Leslie medium. The isolates were submitted to molecular identification by TB Multiplex PCR targeting the 16S rRNA gene and amplicon nucleotide sequencing. From 16 cheese samples (12%), we obtained 26 putative colonies of Mycobacterium spp., none of which belonged to any of the Mycobacterium tuberculosis, Mycobacterium avium, or Mycobacterium intracellulare complexes. Phylogenetic analysis showed that sample sequences were grouped in a clade that includes only non-tuberculous mycobacteria with proximity to sequences obtained from Mycobacterium novocastrense (3 sequences), Mycobacterium holsaticum (1 sequence), andMycobacterium elephantis (2 sequences). Although no epidemiological evidence was found regarding the importance of oral transmission of mycobacteria in healthy people, their importance in the immunosuppressed population remains uncertain.Mycobacterium bovis é o agente da tuberculose bovina, doença que acomete o rebanho em todo território brasileiro e é uma negligenciada zoonose transmitida pelo leite e seus derivados. Este trabalho avaliou a presença de M. bovis e outras micobactérias, em queijo minas meia-cura, obtidos em feiras-livres na cidade de São Paulo, entre os anos de 2012 e 2013. As amostras (n = 133) foram descontaminadas pelo método HPC (hexa-cetyl-pyridinium chloride) e semeadas em meio Stonebrink Leslie. Os isolados foram submetidos à identificação molecular por PCR TB multiplex, pesquisando-se o gene 16S rRNA, e ao sequenciamento nucleotídico. Dezesseis amostras (12%) possuiam 26 colônias sugestivas de Mycobacterium spp., mas nenhuma delas pertencia aos complexos Mycobacterium tuberculosis, Mycobacterium avium e Mycobacterium intracellulare. A análise filogenética mostrou que todas as amostras estavam agrupadas em clados que incluem apenas micobactérias não tuberculosas (MNT), sendo que algumas possuiam proximidade com sequências obtidas de Mycobacterium novocastrense (3 sequências), Mycobacterium hosaticum (1 sequência) e Mycobacterium elephantis (2 sequências). Embora no momento não haja evidência epidemiológica da importância da transmissão oral das micobactérias pra indivíduos saudáveis, sua importância na população imunossuprimida ainda é incerta.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2018-12-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/e14652510.11606/issn.1678-4456.bjvras.2018.146525Brazilian Journal of Veterinary Research and Animal Science; Vol. 55 Núm. 4 (2018); e146525Brazilian Journal of Veterinary Research and Animal Science; Vol. 55 No. 4 (2018); e146525Brazilian Journal of Veterinary Research and Animal Science; v. 55 n. 4 (2018); e146525Brazilian Journal of Veterinary Research and Animal Science; V. 55 N. 4 (2018); e1465251678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/e146525/150058Copyright (c) 2018 Brazilian Journal of Veterinary Research and Animal Scienceinfo:eu-repo/semantics/openAccessMoriconi, Patricia RossiIkuta, Cássia YumiGregori, FábioOliveira, Gisele deOliveira, Sheila deTonietti, Paloma de OliveiraFerreira Neto, José SoaresFerreira, FernandoCortez, AdrianaTelles, Evelise Oliveira2020-06-23T04:02:46Zoai:revistas.usp.br:article/146525Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:44:02.193932Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Mycobacteria in Minas cheese commercialized in open fairs in São Paulo, Brazil Mycobacteria em queijos tipo Minas comercializados em feiras-livres de São Paulo, Brasil |
title |
Mycobacteria in Minas cheese commercialized in open fairs in São Paulo, Brazil |
spellingShingle |
Mycobacteria in Minas cheese commercialized in open fairs in São Paulo, Brazil Moriconi, Patricia Rossi Mycobacteria Non-tuberculous mycobacteria Mycobacterium bovis Minas cheese Free-trade fair Mycobacteria Micobactérias não tuberculosas Mycobacterium bovis Queijo tipo minas Feiras-livres |
title_short |
Mycobacteria in Minas cheese commercialized in open fairs in São Paulo, Brazil |
title_full |
Mycobacteria in Minas cheese commercialized in open fairs in São Paulo, Brazil |
title_fullStr |
Mycobacteria in Minas cheese commercialized in open fairs in São Paulo, Brazil |
title_full_unstemmed |
Mycobacteria in Minas cheese commercialized in open fairs in São Paulo, Brazil |
title_sort |
Mycobacteria in Minas cheese commercialized in open fairs in São Paulo, Brazil |
author |
Moriconi, Patricia Rossi |
author_facet |
Moriconi, Patricia Rossi Ikuta, Cássia Yumi Gregori, Fábio Oliveira, Gisele de Oliveira, Sheila de Tonietti, Paloma de Oliveira Ferreira Neto, José Soares Ferreira, Fernando Cortez, Adriana Telles, Evelise Oliveira |
author_role |
author |
author2 |
Ikuta, Cássia Yumi Gregori, Fábio Oliveira, Gisele de Oliveira, Sheila de Tonietti, Paloma de Oliveira Ferreira Neto, José Soares Ferreira, Fernando Cortez, Adriana Telles, Evelise Oliveira |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Moriconi, Patricia Rossi Ikuta, Cássia Yumi Gregori, Fábio Oliveira, Gisele de Oliveira, Sheila de Tonietti, Paloma de Oliveira Ferreira Neto, José Soares Ferreira, Fernando Cortez, Adriana Telles, Evelise Oliveira |
dc.subject.por.fl_str_mv |
Mycobacteria Non-tuberculous mycobacteria Mycobacterium bovis Minas cheese Free-trade fair Mycobacteria Micobactérias não tuberculosas Mycobacterium bovis Queijo tipo minas Feiras-livres |
topic |
Mycobacteria Non-tuberculous mycobacteria Mycobacterium bovis Minas cheese Free-trade fair Mycobacteria Micobactérias não tuberculosas Mycobacterium bovis Queijo tipo minas Feiras-livres |
description |
Mycobacterium bovis is the causative agent of bovine tuberculosis, a disease that affects dairy herds throughout the Brazilian territory, constituting a neglected zoonosis transmitted by raw milk and its derivatives. In this study, we evaluated the presence of M. bovis and other mycobacteria in Minas cheese obtained from open fairs in the city of São Paulo between 2012 and 2013. Samples (n = 133) were decontaminated using hexa-cetylpyridinium chloride and seeded on Stonebrink–Leslie medium. The isolates were submitted to molecular identification by TB Multiplex PCR targeting the 16S rRNA gene and amplicon nucleotide sequencing. From 16 cheese samples (12%), we obtained 26 putative colonies of Mycobacterium spp., none of which belonged to any of the Mycobacterium tuberculosis, Mycobacterium avium, or Mycobacterium intracellulare complexes. Phylogenetic analysis showed that sample sequences were grouped in a clade that includes only non-tuberculous mycobacteria with proximity to sequences obtained from Mycobacterium novocastrense (3 sequences), Mycobacterium holsaticum (1 sequence), andMycobacterium elephantis (2 sequences). Although no epidemiological evidence was found regarding the importance of oral transmission of mycobacteria in healthy people, their importance in the immunosuppressed population remains uncertain. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-26 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/e146525 10.11606/issn.1678-4456.bjvras.2018.146525 |
url |
https://www.revistas.usp.br/bjvras/article/view/e146525 |
identifier_str_mv |
10.11606/issn.1678-4456.bjvras.2018.146525 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/e146525/150058 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Brazilian Journal of Veterinary Research and Animal Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Brazilian Journal of Veterinary Research and Animal Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 55 Núm. 4 (2018); e146525 Brazilian Journal of Veterinary Research and Animal Science; Vol. 55 No. 4 (2018); e146525 Brazilian Journal of Veterinary Research and Animal Science; v. 55 n. 4 (2018); e146525 Brazilian Journal of Veterinary Research and Animal Science; V. 55 N. 4 (2018); e146525 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051567340257280 |