Selenium supplementation during puberty and young adult-hood mitigates obesity-induced metabolic, cellular and epigenetic alterations in male rat physiology

Detalhes bibliográficos
Autor(a) principal: Pascoal, Gabriela de Freitas Laiber
Data de Publicação: 2022
Tipo de documento: Tese
Idioma: eng
Título da fonte: Biblioteca Digital de Teses e Dissertações da USP
Texto Completo: https://www.teses.usp.br/teses/disponiveis/9/9132/tde-25082022-172904/
Resumo: Selenium (Se) role in obesity is not clear. In addition, information on Ses role in male physiology, specifically in obesity, is scarce. We conducted this study to evaluate the efficacy of Se supplementation, specifically during puberty until young adulthood, against obesity-induced deregulation of metabolic, cellular, and epigenetic parameters in epididymal fat and/or sperm cells in a rat model. High-fat-diet consumption by male rats during puberty and young adulthood significantly increased body weight, adipocyte size, oxidative stress, deregulated expression of genes associated with inflammation (Adiponectin, IL-6, TNF-α), adipogenesis (CEBPα), estrogen biosynthesis (CYP19) and epigenetic processes in epididymal adipose tissue (Dnmt3a), as well as altered microRNA expression vital for spermatogenesis in sperm cells (miR-15b and miR-497). On the other hand, Se supplementation significantly decreased oxidative stress and mitigated these molecular/epigenetic alterations in epididymal adipose tissue or sperm cells. Our results indicate that selenium supplementation during puberty/young adulthood could improve male physiology in the context of obesity. In addition, it suggests that Se could potentially positively affect offspring health
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spelling Selenium supplementation during puberty and young adult-hood mitigates obesity-induced metabolic, cellular and epigenetic alterations in male rat physiologyA suplementação de selênio durante a puberdade e a idade adulta jovem atenua as alterações metabólicas, celulares e epigenéticas induzidas pela obesidade na fisiologia de ratos machosEpigenetics reprogrammingFisiologia reprodutiva masculinaMale reproductive physiologyObesidadeObesityReprogramação epigenéticaSelênioSeleniumSelenium (Se) role in obesity is not clear. In addition, information on Ses role in male physiology, specifically in obesity, is scarce. We conducted this study to evaluate the efficacy of Se supplementation, specifically during puberty until young adulthood, against obesity-induced deregulation of metabolic, cellular, and epigenetic parameters in epididymal fat and/or sperm cells in a rat model. High-fat-diet consumption by male rats during puberty and young adulthood significantly increased body weight, adipocyte size, oxidative stress, deregulated expression of genes associated with inflammation (Adiponectin, IL-6, TNF-α), adipogenesis (CEBPα), estrogen biosynthesis (CYP19) and epigenetic processes in epididymal adipose tissue (Dnmt3a), as well as altered microRNA expression vital for spermatogenesis in sperm cells (miR-15b and miR-497). On the other hand, Se supplementation significantly decreased oxidative stress and mitigated these molecular/epigenetic alterations in epididymal adipose tissue or sperm cells. Our results indicate that selenium supplementation during puberty/young adulthood could improve male physiology in the context of obesity. In addition, it suggests that Se could potentially positively affect offspring healthO papel do selênio (Se) na obesidade não é claro. Além disso, a informação sobre o papel do Se na fisiologia reprodutiva masculina, especificamente na obesidade, é escassa. Conduzimos este estudo para avaliar a eficácia da suplementação de Se especificamente durante a puberdade até à idade adulta jovem contra a desregulação induzida pela obesidade nos parâmetros metabólicos, celulares e epigenéticos na gordura epididimal e/ou nas células espermáticas num modelo de rato. O consumo elevado de gordura por ratos machos durante a puberdade e na idade adulta jovem aumentou significativamente o peso corporal, o tamanho dos adipócitos, o stress oxidativo, a expressão desregulada de genes associados à inflamação (Adiponectina, IL- 6, TNF-α), adipogênese (CEBPα), biossíntese de estrogênio (CYP19) e processos epigenéticos em tecido adiposo epididimal (Dnmt3a), bem como expressão alterada de microRNA vital para a espermatogênese em células espermáticas (miR-15b e miR-497). Por outro lado, a suplementação com Se aumentou a atividade antioxidante, diminuiu o stress oxidativo e mitigou estas alterações moleculares/epigenéticas tanto no tecido adiposo epididimal como nas células espermáticas. Os nossos resultados indicam que a suplementação com selênio durante a puberdade/ idade adulta poderia melhorar a fisiologia reprodutiva masculina num contexto de obesidade. Além disso, sugere que o Se poderia potencialmente afetar positivamente a saúde da descendência.Biblioteca Digitais de Teses e Dissertações da USPOng, Thomas PratesPascoal, Gabriela de Freitas Laiber2022-07-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttps://www.teses.usp.br/teses/disponiveis/9/9132/tde-25082022-172904/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2024-07-08T13:00:13Zoai:teses.usp.br:tde-25082022-172904Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212024-07-08T13:00:13Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Selenium supplementation during puberty and young adult-hood mitigates obesity-induced metabolic, cellular and epigenetic alterations in male rat physiology
A suplementação de selênio durante a puberdade e a idade adulta jovem atenua as alterações metabólicas, celulares e epigenéticas induzidas pela obesidade na fisiologia de ratos machos
title Selenium supplementation during puberty and young adult-hood mitigates obesity-induced metabolic, cellular and epigenetic alterations in male rat physiology
spellingShingle Selenium supplementation during puberty and young adult-hood mitigates obesity-induced metabolic, cellular and epigenetic alterations in male rat physiology
Pascoal, Gabriela de Freitas Laiber
Epigenetics reprogramming
Fisiologia reprodutiva masculina
Male reproductive physiology
Obesidade
Obesity
Reprogramação epigenética
Selênio
Selenium
title_short Selenium supplementation during puberty and young adult-hood mitigates obesity-induced metabolic, cellular and epigenetic alterations in male rat physiology
title_full Selenium supplementation during puberty and young adult-hood mitigates obesity-induced metabolic, cellular and epigenetic alterations in male rat physiology
title_fullStr Selenium supplementation during puberty and young adult-hood mitigates obesity-induced metabolic, cellular and epigenetic alterations in male rat physiology
title_full_unstemmed Selenium supplementation during puberty and young adult-hood mitigates obesity-induced metabolic, cellular and epigenetic alterations in male rat physiology
title_sort Selenium supplementation during puberty and young adult-hood mitigates obesity-induced metabolic, cellular and epigenetic alterations in male rat physiology
author Pascoal, Gabriela de Freitas Laiber
author_facet Pascoal, Gabriela de Freitas Laiber
author_role author
dc.contributor.none.fl_str_mv Ong, Thomas Prates
dc.contributor.author.fl_str_mv Pascoal, Gabriela de Freitas Laiber
dc.subject.por.fl_str_mv Epigenetics reprogramming
Fisiologia reprodutiva masculina
Male reproductive physiology
Obesidade
Obesity
Reprogramação epigenética
Selênio
Selenium
topic Epigenetics reprogramming
Fisiologia reprodutiva masculina
Male reproductive physiology
Obesidade
Obesity
Reprogramação epigenética
Selênio
Selenium
description Selenium (Se) role in obesity is not clear. In addition, information on Ses role in male physiology, specifically in obesity, is scarce. We conducted this study to evaluate the efficacy of Se supplementation, specifically during puberty until young adulthood, against obesity-induced deregulation of metabolic, cellular, and epigenetic parameters in epididymal fat and/or sperm cells in a rat model. High-fat-diet consumption by male rats during puberty and young adulthood significantly increased body weight, adipocyte size, oxidative stress, deregulated expression of genes associated with inflammation (Adiponectin, IL-6, TNF-α), adipogenesis (CEBPα), estrogen biosynthesis (CYP19) and epigenetic processes in epididymal adipose tissue (Dnmt3a), as well as altered microRNA expression vital for spermatogenesis in sperm cells (miR-15b and miR-497). On the other hand, Se supplementation significantly decreased oxidative stress and mitigated these molecular/epigenetic alterations in epididymal adipose tissue or sperm cells. Our results indicate that selenium supplementation during puberty/young adulthood could improve male physiology in the context of obesity. In addition, it suggests that Se could potentially positively affect offspring health
publishDate 2022
dc.date.none.fl_str_mv 2022-07-08
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.teses.usp.br/teses/disponiveis/9/9132/tde-25082022-172904/
url https://www.teses.usp.br/teses/disponiveis/9/9132/tde-25082022-172904/
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv
dc.rights.driver.fl_str_mv Liberar o conteúdo para acesso público.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Liberar o conteúdo para acesso público.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv
dc.publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
dc.source.none.fl_str_mv
reponame:Biblioteca Digital de Teses e Dissertações da USP
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Biblioteca Digital de Teses e Dissertações da USP
collection Biblioteca Digital de Teses e Dissertações da USP
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)
repository.mail.fl_str_mv virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br
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