Somatic embryogenesis from leaf tissues of macaw palm [Acrocomia aculeata (Jacq.) Lodd. ex Mart.]
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000501019 |
Resumo: | Abstract A somatic embryogenesis protocol was developed from the immature leaves of adult plants of the macaw palm. Leaf explants from different regions of the palm heart were used for callus initiation in a modified Y3 medium, supplemented with 2,4-D or Picloram at 450 μM. Calli were separated from the leaf explants at 6-, 9- and 12-month periods and transferred to a fresh culture medium of the same composition. They were multiplied for up to 120 days. Reduced concentrations of 2,4-D and Picloram were used to differentiate somatic embryos. They were then germinated in a medium without plant growth regulators. Morphological and anatomical analyses were conducted at different stages of the embryogenic process. The best results for callus induction were achieved by Picloram, when explants were maintained for up to 9 months on culture medium (64.9%). The farthest portions of the apical meristem were those that provided the biggest calli formation. The formation of the somatic embryos was observed from the calli multiplication phase. Reduction in concentrations of growth regulators failed to promote the formation of complete plants. Picloram at 450 μM promotes high callogenesis in leaf tissues of macaw palm, with a potential for somatic embryo formation. |
id |
ABC-1_330255fcb693ecae4ac3861c2c780f16 |
---|---|
oai_identifier_str |
oai:scielo:S0001-37652020000501019 |
network_acronym_str |
ABC-1 |
network_name_str |
Anais da Academia Brasileira de Ciências (Online) |
repository_id_str |
|
spelling |
Somatic embryogenesis from leaf tissues of macaw palm [Acrocomia aculeata (Jacq.) Lodd. ex Mart.]Arecaceaesomatic embryogenesisleafcallus culturewoody plantsAbstract A somatic embryogenesis protocol was developed from the immature leaves of adult plants of the macaw palm. Leaf explants from different regions of the palm heart were used for callus initiation in a modified Y3 medium, supplemented with 2,4-D or Picloram at 450 μM. Calli were separated from the leaf explants at 6-, 9- and 12-month periods and transferred to a fresh culture medium of the same composition. They were multiplied for up to 120 days. Reduced concentrations of 2,4-D and Picloram were used to differentiate somatic embryos. They were then germinated in a medium without plant growth regulators. Morphological and anatomical analyses were conducted at different stages of the embryogenic process. The best results for callus induction were achieved by Picloram, when explants were maintained for up to 9 months on culture medium (64.9%). The farthest portions of the apical meristem were those that provided the biggest calli formation. The formation of the somatic embryos was observed from the calli multiplication phase. Reduction in concentrations of growth regulators failed to promote the formation of complete plants. Picloram at 450 μM promotes high callogenesis in leaf tissues of macaw palm, with a potential for somatic embryo formation.Academia Brasileira de Ciências2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000501019Anais da Academia Brasileira de Ciências v.92 n.3 2020reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765202020180709info:eu-repo/semantics/openAccessMEIRA,FILIPE S.LUIS,ZANDERLUCE G.CARDOSO,INAÊ MARIÊ A.S.SCHERWINSKI-PEREIRA,JONNY E.eng2020-11-09T00:00:00Zoai:scielo:S0001-37652020000501019Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2020-11-09T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Somatic embryogenesis from leaf tissues of macaw palm [Acrocomia aculeata (Jacq.) Lodd. ex Mart.] |
title |
Somatic embryogenesis from leaf tissues of macaw palm [Acrocomia aculeata (Jacq.) Lodd. ex Mart.] |
spellingShingle |
Somatic embryogenesis from leaf tissues of macaw palm [Acrocomia aculeata (Jacq.) Lodd. ex Mart.] MEIRA,FILIPE S. Arecaceae somatic embryogenesis leaf callus culture woody plants |
title_short |
Somatic embryogenesis from leaf tissues of macaw palm [Acrocomia aculeata (Jacq.) Lodd. ex Mart.] |
title_full |
Somatic embryogenesis from leaf tissues of macaw palm [Acrocomia aculeata (Jacq.) Lodd. ex Mart.] |
title_fullStr |
Somatic embryogenesis from leaf tissues of macaw palm [Acrocomia aculeata (Jacq.) Lodd. ex Mart.] |
title_full_unstemmed |
Somatic embryogenesis from leaf tissues of macaw palm [Acrocomia aculeata (Jacq.) Lodd. ex Mart.] |
title_sort |
Somatic embryogenesis from leaf tissues of macaw palm [Acrocomia aculeata (Jacq.) Lodd. ex Mart.] |
author |
MEIRA,FILIPE S. |
author_facet |
MEIRA,FILIPE S. LUIS,ZANDERLUCE G. CARDOSO,INAÊ MARIÊ A.S. SCHERWINSKI-PEREIRA,JONNY E. |
author_role |
author |
author2 |
LUIS,ZANDERLUCE G. CARDOSO,INAÊ MARIÊ A.S. SCHERWINSKI-PEREIRA,JONNY E. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
MEIRA,FILIPE S. LUIS,ZANDERLUCE G. CARDOSO,INAÊ MARIÊ A.S. SCHERWINSKI-PEREIRA,JONNY E. |
dc.subject.por.fl_str_mv |
Arecaceae somatic embryogenesis leaf callus culture woody plants |
topic |
Arecaceae somatic embryogenesis leaf callus culture woody plants |
description |
Abstract A somatic embryogenesis protocol was developed from the immature leaves of adult plants of the macaw palm. Leaf explants from different regions of the palm heart were used for callus initiation in a modified Y3 medium, supplemented with 2,4-D or Picloram at 450 μM. Calli were separated from the leaf explants at 6-, 9- and 12-month periods and transferred to a fresh culture medium of the same composition. They were multiplied for up to 120 days. Reduced concentrations of 2,4-D and Picloram were used to differentiate somatic embryos. They were then germinated in a medium without plant growth regulators. Morphological and anatomical analyses were conducted at different stages of the embryogenic process. The best results for callus induction were achieved by Picloram, when explants were maintained for up to 9 months on culture medium (64.9%). The farthest portions of the apical meristem were those that provided the biggest calli formation. The formation of the somatic embryos was observed from the calli multiplication phase. Reduction in concentrations of growth regulators failed to promote the formation of complete plants. Picloram at 450 μM promotes high callogenesis in leaf tissues of macaw palm, with a potential for somatic embryo formation. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000501019 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000501019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0001-3765202020180709 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.92 n.3 2020 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
_version_ |
1754302869329149952 |