Associative diazotrophic bacteria in grass roots and soils from heavy metal contaminated sites
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652008000400014 |
Resumo: | This work aimed to evaluate density of associative diazotrophic bacteria populations in soil and grass root samples from heavy metal contaminated sites, and to characterize isolates from these populations, both, phenotypically (Zinc, Cadmium and NaCl tolerance in vitro, and protein profiles) and genotypically (16S rDNA sequencing), as compared to type strains of known diazotrophic species. Densities were evaluated by using NFb, Fam and JNFb media, commonly used for enrichment cultures of diazotrophic bacteria. Bacterial densities found in soil and grass root samples from contaminated sites were similar to those reported for agricultural soils. Azospirillum spp. isolates from contaminated sites and type strains from non-contaminated sites varied substantially in their in vitro tolerance to Zn+2 and Cd+2, being Cd+2 more toxic than Zn+2. Among the most tolerant isolates (UFLA 1S, 1R, S181, S34 and S22), some (1R, S34 and S22) were more tolerant to heavy metals than rhizobia from tropical and temperate soils. The majority of the isolates tolerant to heavy metals were also tolerant to salt stress as indicated by their ability to grow in solid medium supplemented with 30 g L-1 NaCl. Five isolates exhibited high dissimilarity in protein profiles, and the 16S rDNA sequence analysis of two of them revealed new sequences for Azospirillum. |
id |
ABC-1_8199b51b6fd6a3005d9729456b445b93 |
---|---|
oai_identifier_str |
oai:scielo:S0001-37652008000400014 |
network_acronym_str |
ABC-1 |
network_name_str |
Anais da Academia Brasileira de Ciências (Online) |
repository_id_str |
|
spelling |
Associative diazotrophic bacteria in grass roots and soils from heavy metal contaminated sitesAzospirillumZincCadmiumsoil contaminantsNitrogen-fixing bacteriaThis work aimed to evaluate density of associative diazotrophic bacteria populations in soil and grass root samples from heavy metal contaminated sites, and to characterize isolates from these populations, both, phenotypically (Zinc, Cadmium and NaCl tolerance in vitro, and protein profiles) and genotypically (16S rDNA sequencing), as compared to type strains of known diazotrophic species. Densities were evaluated by using NFb, Fam and JNFb media, commonly used for enrichment cultures of diazotrophic bacteria. Bacterial densities found in soil and grass root samples from contaminated sites were similar to those reported for agricultural soils. Azospirillum spp. isolates from contaminated sites and type strains from non-contaminated sites varied substantially in their in vitro tolerance to Zn+2 and Cd+2, being Cd+2 more toxic than Zn+2. Among the most tolerant isolates (UFLA 1S, 1R, S181, S34 and S22), some (1R, S34 and S22) were more tolerant to heavy metals than rhizobia from tropical and temperate soils. The majority of the isolates tolerant to heavy metals were also tolerant to salt stress as indicated by their ability to grow in solid medium supplemented with 30 g L-1 NaCl. Five isolates exhibited high dissimilarity in protein profiles, and the 16S rDNA sequence analysis of two of them revealed new sequences for Azospirillum.Academia Brasileira de Ciências2008-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652008000400014Anais da Academia Brasileira de Ciências v.80 n.4 2008reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/S0001-37652008000400014info:eu-repo/semantics/openAccessMoreira,Fátima M.S.Lange,AndersonKlauberg-Filho,OsmarSiqueira,José O.Nóbrega,Rafaela S.A.Lima,Adriana S.eng2008-11-25T00:00:00Zoai:scielo:S0001-37652008000400014Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2008-11-25T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Associative diazotrophic bacteria in grass roots and soils from heavy metal contaminated sites |
title |
Associative diazotrophic bacteria in grass roots and soils from heavy metal contaminated sites |
spellingShingle |
Associative diazotrophic bacteria in grass roots and soils from heavy metal contaminated sites Moreira,Fátima M.S. Azospirillum Zinc Cadmium soil contaminants Nitrogen-fixing bacteria |
title_short |
Associative diazotrophic bacteria in grass roots and soils from heavy metal contaminated sites |
title_full |
Associative diazotrophic bacteria in grass roots and soils from heavy metal contaminated sites |
title_fullStr |
Associative diazotrophic bacteria in grass roots and soils from heavy metal contaminated sites |
title_full_unstemmed |
Associative diazotrophic bacteria in grass roots and soils from heavy metal contaminated sites |
title_sort |
Associative diazotrophic bacteria in grass roots and soils from heavy metal contaminated sites |
author |
Moreira,Fátima M.S. |
author_facet |
Moreira,Fátima M.S. Lange,Anderson Klauberg-Filho,Osmar Siqueira,José O. Nóbrega,Rafaela S.A. Lima,Adriana S. |
author_role |
author |
author2 |
Lange,Anderson Klauberg-Filho,Osmar Siqueira,José O. Nóbrega,Rafaela S.A. Lima,Adriana S. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Moreira,Fátima M.S. Lange,Anderson Klauberg-Filho,Osmar Siqueira,José O. Nóbrega,Rafaela S.A. Lima,Adriana S. |
dc.subject.por.fl_str_mv |
Azospirillum Zinc Cadmium soil contaminants Nitrogen-fixing bacteria |
topic |
Azospirillum Zinc Cadmium soil contaminants Nitrogen-fixing bacteria |
description |
This work aimed to evaluate density of associative diazotrophic bacteria populations in soil and grass root samples from heavy metal contaminated sites, and to characterize isolates from these populations, both, phenotypically (Zinc, Cadmium and NaCl tolerance in vitro, and protein profiles) and genotypically (16S rDNA sequencing), as compared to type strains of known diazotrophic species. Densities were evaluated by using NFb, Fam and JNFb media, commonly used for enrichment cultures of diazotrophic bacteria. Bacterial densities found in soil and grass root samples from contaminated sites were similar to those reported for agricultural soils. Azospirillum spp. isolates from contaminated sites and type strains from non-contaminated sites varied substantially in their in vitro tolerance to Zn+2 and Cd+2, being Cd+2 more toxic than Zn+2. Among the most tolerant isolates (UFLA 1S, 1R, S181, S34 and S22), some (1R, S34 and S22) were more tolerant to heavy metals than rhizobia from tropical and temperate soils. The majority of the isolates tolerant to heavy metals were also tolerant to salt stress as indicated by their ability to grow in solid medium supplemented with 30 g L-1 NaCl. Five isolates exhibited high dissimilarity in protein profiles, and the 16S rDNA sequence analysis of two of them revealed new sequences for Azospirillum. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652008000400014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652008000400014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0001-37652008000400014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.80 n.4 2008 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
_version_ |
1754302857217048576 |