The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps

Detalhes bibliográficos
Autor(a) principal: MARTINELLI,ARTURO B.
Data de Publicação: 2017
Outros Autores: WAICHERT,CECILIA, BARBOSA,DIEGO N., FAGUNDES,VALÉRIA, AZEVEDO,CELSO O.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401629
Resumo: ABSTRACT Genitalia are rich source of characters in insect taxonomy. Usually, they are examined after dissection and cleaning with potassium hydroxide (KOH), procedure that may damage both genital morphological structures and intracellular molecular contents. Enzymatic procedure with Proteinase K has been used to clean muscle off the genitalia while extract DNA, but its damage to the genital structures has not been evaluated. Herein, we qualitatively compare the use of KOH and Proteinase K to prepare genital structures in minute insects (Hymenoptera: Bethylidae). We show that Proteinase K is better to preserve the genital structure and provides quality DNA.
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spelling The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute waspsDiaphanizationgenitalia preparationKOHWolbachiaABSTRACT Genitalia are rich source of characters in insect taxonomy. Usually, they are examined after dissection and cleaning with potassium hydroxide (KOH), procedure that may damage both genital morphological structures and intracellular molecular contents. Enzymatic procedure with Proteinase K has been used to clean muscle off the genitalia while extract DNA, but its damage to the genital structures has not been evaluated. Herein, we qualitatively compare the use of KOH and Proteinase K to prepare genital structures in minute insects (Hymenoptera: Bethylidae). We show that Proteinase K is better to preserve the genital structure and provides quality DNA.Academia Brasileira de Ciências2017-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401629Anais da Academia Brasileira de Ciências v.89 n.3 2017reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201720160825info:eu-repo/semantics/openAccessMARTINELLI,ARTURO B.WAICHERT,CECILIABARBOSA,DIEGO N.FAGUNDES,VALÉRIAAZEVEDO,CELSO O.eng2019-11-29T00:00:00Zoai:scielo:S0001-37652017000401629Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2019-11-29T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps
title The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps
spellingShingle The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps
MARTINELLI,ARTURO B.
Diaphanization
genitalia preparation
KOH
Wolbachia
title_short The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps
title_full The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps
title_fullStr The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps
title_full_unstemmed The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps
title_sort The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps
author MARTINELLI,ARTURO B.
author_facet MARTINELLI,ARTURO B.
WAICHERT,CECILIA
BARBOSA,DIEGO N.
FAGUNDES,VALÉRIA
AZEVEDO,CELSO O.
author_role author
author2 WAICHERT,CECILIA
BARBOSA,DIEGO N.
FAGUNDES,VALÉRIA
AZEVEDO,CELSO O.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv MARTINELLI,ARTURO B.
WAICHERT,CECILIA
BARBOSA,DIEGO N.
FAGUNDES,VALÉRIA
AZEVEDO,CELSO O.
dc.subject.por.fl_str_mv Diaphanization
genitalia preparation
KOH
Wolbachia
topic Diaphanization
genitalia preparation
KOH
Wolbachia
description ABSTRACT Genitalia are rich source of characters in insect taxonomy. Usually, they are examined after dissection and cleaning with potassium hydroxide (KOH), procedure that may damage both genital morphological structures and intracellular molecular contents. Enzymatic procedure with Proteinase K has been used to clean muscle off the genitalia while extract DNA, but its damage to the genital structures has not been evaluated. Herein, we qualitatively compare the use of KOH and Proteinase K to prepare genital structures in minute insects (Hymenoptera: Bethylidae). We show that Proteinase K is better to preserve the genital structure and provides quality DNA.
publishDate 2017
dc.date.none.fl_str_mv 2017-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401629
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401629
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765201720160825
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.89 n.3 2017
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
instacron_str ABC
institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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