Production, characterization and application of inulinase from fungal endophyte CCMB 328
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200014 |
Resumo: | Inulinase (β-2,1-D- fructan fructanohydrolase), EC 3.2.1.7, targets the β-2,1 linkage of inulin, a polyfructan consisting of linear β-2,1 linked fructose, and hydrolyzes it into fructose. This use provides an alternative to produce fructose syrup through the hydrolysis of inulin. The objective of this work was to study the production, characterization and applications of inulinases from the fungal endophyte CCMB 328 isolated from the Brazilian semi-arid region. Response Surface Methodology (RSM) was employed to evaluate the effect of variables (concentration of glucose and yeast extract), on secreted inulinase activities detected in the culture medium and also in the inulin hydrolysis. The results showed that the best conditions for inulinase production by CCMB 328 are 9.89 g / L for glucose and 1.09 g / L for yeast extract. The concentration of 0.20 mol/L of NaCl and KCl increased the activity of inulinase from CCMB 328 by approximately 63% and 37%, respectively. The results also showed that the inulinase has potential for inulin hydrolysis, whose conversion yields roughly 72.48 % for an initial concentration of inulin at 1% (w/v). |
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Production, characterization and application of inulinase from fungal endophyte CCMB 328Inulininulinaseyeastssemi-aridfungal endophyteInulinase (β-2,1-D- fructan fructanohydrolase), EC 3.2.1.7, targets the β-2,1 linkage of inulin, a polyfructan consisting of linear β-2,1 linked fructose, and hydrolyzes it into fructose. This use provides an alternative to produce fructose syrup through the hydrolysis of inulin. The objective of this work was to study the production, characterization and applications of inulinases from the fungal endophyte CCMB 328 isolated from the Brazilian semi-arid region. Response Surface Methodology (RSM) was employed to evaluate the effect of variables (concentration of glucose and yeast extract), on secreted inulinase activities detected in the culture medium and also in the inulin hydrolysis. The results showed that the best conditions for inulinase production by CCMB 328 are 9.89 g / L for glucose and 1.09 g / L for yeast extract. The concentration of 0.20 mol/L of NaCl and KCl increased the activity of inulinase from CCMB 328 by approximately 63% and 37%, respectively. The results also showed that the inulinase has potential for inulin hydrolysis, whose conversion yields roughly 72.48 % for an initial concentration of inulin at 1% (w/v).Academia Brasileira de Ciências2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200014Anais da Academia Brasileira de Ciências v.84 n.2 2012reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/S0001-37652012005000035info:eu-repo/semantics/openAccessNascimento,Diego S.Valasques Junior,GildomarFernandes,PedroRibeiro,Geise C.A.Lima,Danyo M.Góes-Neto,AristótelesOliveira,Rodrigo Q.Figueiredo-Ribeiro,Rita de Cassia L.Assis,Sandra A. deeng2012-05-25T00:00:00Zoai:scielo:S0001-37652012000200014Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2012-05-25T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Production, characterization and application of inulinase from fungal endophyte CCMB 328 |
title |
Production, characterization and application of inulinase from fungal endophyte CCMB 328 |
spellingShingle |
Production, characterization and application of inulinase from fungal endophyte CCMB 328 Nascimento,Diego S. Inulin inulinase yeasts semi-arid fungal endophyte |
title_short |
Production, characterization and application of inulinase from fungal endophyte CCMB 328 |
title_full |
Production, characterization and application of inulinase from fungal endophyte CCMB 328 |
title_fullStr |
Production, characterization and application of inulinase from fungal endophyte CCMB 328 |
title_full_unstemmed |
Production, characterization and application of inulinase from fungal endophyte CCMB 328 |
title_sort |
Production, characterization and application of inulinase from fungal endophyte CCMB 328 |
author |
Nascimento,Diego S. |
author_facet |
Nascimento,Diego S. Valasques Junior,Gildomar Fernandes,Pedro Ribeiro,Geise C.A. Lima,Danyo M. Góes-Neto,Aristóteles Oliveira,Rodrigo Q. Figueiredo-Ribeiro,Rita de Cassia L. Assis,Sandra A. de |
author_role |
author |
author2 |
Valasques Junior,Gildomar Fernandes,Pedro Ribeiro,Geise C.A. Lima,Danyo M. Góes-Neto,Aristóteles Oliveira,Rodrigo Q. Figueiredo-Ribeiro,Rita de Cassia L. Assis,Sandra A. de |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Nascimento,Diego S. Valasques Junior,Gildomar Fernandes,Pedro Ribeiro,Geise C.A. Lima,Danyo M. Góes-Neto,Aristóteles Oliveira,Rodrigo Q. Figueiredo-Ribeiro,Rita de Cassia L. Assis,Sandra A. de |
dc.subject.por.fl_str_mv |
Inulin inulinase yeasts semi-arid fungal endophyte |
topic |
Inulin inulinase yeasts semi-arid fungal endophyte |
description |
Inulinase (β-2,1-D- fructan fructanohydrolase), EC 3.2.1.7, targets the β-2,1 linkage of inulin, a polyfructan consisting of linear β-2,1 linked fructose, and hydrolyzes it into fructose. This use provides an alternative to produce fructose syrup through the hydrolysis of inulin. The objective of this work was to study the production, characterization and applications of inulinases from the fungal endophyte CCMB 328 isolated from the Brazilian semi-arid region. Response Surface Methodology (RSM) was employed to evaluate the effect of variables (concentration of glucose and yeast extract), on secreted inulinase activities detected in the culture medium and also in the inulin hydrolysis. The results showed that the best conditions for inulinase production by CCMB 328 are 9.89 g / L for glucose and 1.09 g / L for yeast extract. The concentration of 0.20 mol/L of NaCl and KCl increased the activity of inulinase from CCMB 328 by approximately 63% and 37%, respectively. The results also showed that the inulinase has potential for inulin hydrolysis, whose conversion yields roughly 72.48 % for an initial concentration of inulin at 1% (w/v). |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0001-37652012005000035 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.84 n.2 2012 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
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1754302858847584256 |