Cytogenotoxicity and protective effect of piperine and capsaicin on meristematic cells of Allium cepa L.
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000600804 |
Resumo: | Abstract Piperine and capsaicin are important molecules with biological and pharmacological activities. This study aimed to evaluate the cytogenotoxic and protective effect of piperine and capsaicin on Allium cepa cells. A. cepa roots were exposed to negative (2% Dimethylsulfoxide) and positive (Methylmethanesulfonate, MMS, 10 µg/mL) controls, and four concentrations (25-200 µM) of piperine or capsaicin (alone) or associated before, simultaneously or after with the MMS. Only the lowest concentration of piperine (25 µM) showed a protective effect because it was not genotoxic. Piperine and capsaicin were cytotoxic (50, 100 and 200 µM). Piperine (50 to 200 µM) caused a significant increase in the total average of chromosomal alterations of in A. cepa cells. For capsaicin, the genotoxic effect was dose-dependent with a significant increase for all concentrations, highlighting the significant presence of micronuclei and nuclear buds for the two isolates. In general, bioactive compounds reduced the total average of chromosomal alterations against damage caused by MMS, mainly micronuclei and/or nuclear buds. Therefore, the two molecules were cytotoxic and genotoxic at the highest concentrations, and did not have cytoprotective action, and the lowest concentration of piperine demonstrated important chemopreventive activity. |
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Cytogenotoxicity and protective effect of piperine and capsaicin on meristematic cells of Allium cepa L.Bioactive compoundsmitotic indexchromosomal alterationsprotection against cell damageAbstract Piperine and capsaicin are important molecules with biological and pharmacological activities. This study aimed to evaluate the cytogenotoxic and protective effect of piperine and capsaicin on Allium cepa cells. A. cepa roots were exposed to negative (2% Dimethylsulfoxide) and positive (Methylmethanesulfonate, MMS, 10 µg/mL) controls, and four concentrations (25-200 µM) of piperine or capsaicin (alone) or associated before, simultaneously or after with the MMS. Only the lowest concentration of piperine (25 µM) showed a protective effect because it was not genotoxic. Piperine and capsaicin were cytotoxic (50, 100 and 200 µM). Piperine (50 to 200 µM) caused a significant increase in the total average of chromosomal alterations of in A. cepa cells. For capsaicin, the genotoxic effect was dose-dependent with a significant increase for all concentrations, highlighting the significant presence of micronuclei and nuclear buds for the two isolates. In general, bioactive compounds reduced the total average of chromosomal alterations against damage caused by MMS, mainly micronuclei and/or nuclear buds. Therefore, the two molecules were cytotoxic and genotoxic at the highest concentrations, and did not have cytoprotective action, and the lowest concentration of piperine demonstrated important chemopreventive activity.Academia Brasileira de Ciências2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000600804Anais da Academia Brasileira de Ciências v.93 suppl.3 2021reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765202120201772info:eu-repo/semantics/openAccessDIAS,MARCONDES S.JUNIOR,ERASMO P.V.SANTOS,BIANCA C. DOSMARTINS,FRANCIELLE A.ALMEIDA,PEDRO M. DEPERON,ANA P.eng2021-09-17T00:00:00Zoai:scielo:S0001-37652021000600804Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2021-09-17T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Cytogenotoxicity and protective effect of piperine and capsaicin on meristematic cells of Allium cepa L. |
title |
Cytogenotoxicity and protective effect of piperine and capsaicin on meristematic cells of Allium cepa L. |
spellingShingle |
Cytogenotoxicity and protective effect of piperine and capsaicin on meristematic cells of Allium cepa L. DIAS,MARCONDES S. Bioactive compounds mitotic index chromosomal alterations protection against cell damage |
title_short |
Cytogenotoxicity and protective effect of piperine and capsaicin on meristematic cells of Allium cepa L. |
title_full |
Cytogenotoxicity and protective effect of piperine and capsaicin on meristematic cells of Allium cepa L. |
title_fullStr |
Cytogenotoxicity and protective effect of piperine and capsaicin on meristematic cells of Allium cepa L. |
title_full_unstemmed |
Cytogenotoxicity and protective effect of piperine and capsaicin on meristematic cells of Allium cepa L. |
title_sort |
Cytogenotoxicity and protective effect of piperine and capsaicin on meristematic cells of Allium cepa L. |
author |
DIAS,MARCONDES S. |
author_facet |
DIAS,MARCONDES S. JUNIOR,ERASMO P.V. SANTOS,BIANCA C. DOS MARTINS,FRANCIELLE A. ALMEIDA,PEDRO M. DE PERON,ANA P. |
author_role |
author |
author2 |
JUNIOR,ERASMO P.V. SANTOS,BIANCA C. DOS MARTINS,FRANCIELLE A. ALMEIDA,PEDRO M. DE PERON,ANA P. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
DIAS,MARCONDES S. JUNIOR,ERASMO P.V. SANTOS,BIANCA C. DOS MARTINS,FRANCIELLE A. ALMEIDA,PEDRO M. DE PERON,ANA P. |
dc.subject.por.fl_str_mv |
Bioactive compounds mitotic index chromosomal alterations protection against cell damage |
topic |
Bioactive compounds mitotic index chromosomal alterations protection against cell damage |
description |
Abstract Piperine and capsaicin are important molecules with biological and pharmacological activities. This study aimed to evaluate the cytogenotoxic and protective effect of piperine and capsaicin on Allium cepa cells. A. cepa roots were exposed to negative (2% Dimethylsulfoxide) and positive (Methylmethanesulfonate, MMS, 10 µg/mL) controls, and four concentrations (25-200 µM) of piperine or capsaicin (alone) or associated before, simultaneously or after with the MMS. Only the lowest concentration of piperine (25 µM) showed a protective effect because it was not genotoxic. Piperine and capsaicin were cytotoxic (50, 100 and 200 µM). Piperine (50 to 200 µM) caused a significant increase in the total average of chromosomal alterations of in A. cepa cells. For capsaicin, the genotoxic effect was dose-dependent with a significant increase for all concentrations, highlighting the significant presence of micronuclei and nuclear buds for the two isolates. In general, bioactive compounds reduced the total average of chromosomal alterations against damage caused by MMS, mainly micronuclei and/or nuclear buds. Therefore, the two molecules were cytotoxic and genotoxic at the highest concentrations, and did not have cytoprotective action, and the lowest concentration of piperine demonstrated important chemopreventive activity. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000600804 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000600804 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0001-3765202120201772 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.93 suppl.3 2021 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
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1754302870536060928 |