Production, characterization and toxicology assay of creatine pegylated nanoliposome with polysorbate 80 for brain delivery
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502317 |
Resumo: | ABSTRACT Creatine acts intracellularly as energy buffer and storage, demonstrating protective effects in animal models of neurodegenerative diseases. However, its permeability throught blood-brain barrier (BBB) is reduced. The aim of the present study was developing a carrier to facilitate the delivery of creatine to the central nervous system. Creatine nanoliposomes were produced, characterized and assayed in models of toxicity in vitro and in vivo. Particles showed negative zeta potential (-12,5 mV), polydispersity index 0.237 and medium-size of 105 nm, which was confirmed by transmission electron microscopy (TEM) images. Toxicity assay in vitro was evaluated with blank liposomes (no drug) or creatine nanoliposomes at concentrations of 0.02 and 0.2 mg/mL, that did not influence the viability of Vero cells. The result. of the comet assay that the nanoliposomes are not genotoxic, togeher with cell viability demonstrated that the nanoliposomes are not toxic. Besides, in vivo assays not demonstrate toxicity in hematological and biochemical markers of young rats. Nevertheless, increase content of creatine in the cerebral cortex tissue after subchronic treatment was observed. Altogether, results indicate increase permeability of creatine to the BBB that could be used as assay for in vivo studies to confirm improved effect than free creatine. |
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Anais da Academia Brasileira de Ciências (Online) |
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Production, characterization and toxicology assay of creatine pegylated nanoliposome with polysorbate 80 for brain deliverynanocarrierneurodegenerative diseasesnanotechnologyliposomesABSTRACT Creatine acts intracellularly as energy buffer and storage, demonstrating protective effects in animal models of neurodegenerative diseases. However, its permeability throught blood-brain barrier (BBB) is reduced. The aim of the present study was developing a carrier to facilitate the delivery of creatine to the central nervous system. Creatine nanoliposomes were produced, characterized and assayed in models of toxicity in vitro and in vivo. Particles showed negative zeta potential (-12,5 mV), polydispersity index 0.237 and medium-size of 105 nm, which was confirmed by transmission electron microscopy (TEM) images. Toxicity assay in vitro was evaluated with blank liposomes (no drug) or creatine nanoliposomes at concentrations of 0.02 and 0.2 mg/mL, that did not influence the viability of Vero cells. The result. of the comet assay that the nanoliposomes are not genotoxic, togeher with cell viability demonstrated that the nanoliposomes are not toxic. Besides, in vivo assays not demonstrate toxicity in hematological and biochemical markers of young rats. Nevertheless, increase content of creatine in the cerebral cortex tissue after subchronic treatment was observed. Altogether, results indicate increase permeability of creatine to the BBB that could be used as assay for in vivo studies to confirm improved effect than free creatine.Academia Brasileira de Ciências2018-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502317Anais da Academia Brasileira de Ciências v.90 n.2 suppl.1 2018reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201820170553info:eu-repo/semantics/openAccessBORIN,DIEGO B.MEZZOMO,NATHANA J.VAUCHER,RODRIGO A.CARMO,GUILHERME DORODRIGUES JUNIOR,LUIZ C.SULCZEWSKI,FERNANDO B.SCHWERTZ,CLAITON I.MENDES,RICARDO E.DAMIANI,ADRIANI P.ANDRADE,VANESSA M. DERECH,VIRGÍNIA C.BOECK,CARINA R.eng2019-11-29T00:00:00Zoai:scielo:S0001-37652018000502317Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2019-11-29T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Production, characterization and toxicology assay of creatine pegylated nanoliposome with polysorbate 80 for brain delivery |
title |
Production, characterization and toxicology assay of creatine pegylated nanoliposome with polysorbate 80 for brain delivery |
spellingShingle |
Production, characterization and toxicology assay of creatine pegylated nanoliposome with polysorbate 80 for brain delivery BORIN,DIEGO B. nanocarrier neurodegenerative diseases nanotechnology liposomes |
title_short |
Production, characterization and toxicology assay of creatine pegylated nanoliposome with polysorbate 80 for brain delivery |
title_full |
Production, characterization and toxicology assay of creatine pegylated nanoliposome with polysorbate 80 for brain delivery |
title_fullStr |
Production, characterization and toxicology assay of creatine pegylated nanoliposome with polysorbate 80 for brain delivery |
title_full_unstemmed |
Production, characterization and toxicology assay of creatine pegylated nanoliposome with polysorbate 80 for brain delivery |
title_sort |
Production, characterization and toxicology assay of creatine pegylated nanoliposome with polysorbate 80 for brain delivery |
author |
BORIN,DIEGO B. |
author_facet |
BORIN,DIEGO B. MEZZOMO,NATHANA J. VAUCHER,RODRIGO A. CARMO,GUILHERME DO RODRIGUES JUNIOR,LUIZ C. SULCZEWSKI,FERNANDO B. SCHWERTZ,CLAITON I. MENDES,RICARDO E. DAMIANI,ADRIANI P. ANDRADE,VANESSA M. DE RECH,VIRGÍNIA C. BOECK,CARINA R. |
author_role |
author |
author2 |
MEZZOMO,NATHANA J. VAUCHER,RODRIGO A. CARMO,GUILHERME DO RODRIGUES JUNIOR,LUIZ C. SULCZEWSKI,FERNANDO B. SCHWERTZ,CLAITON I. MENDES,RICARDO E. DAMIANI,ADRIANI P. ANDRADE,VANESSA M. DE RECH,VIRGÍNIA C. BOECK,CARINA R. |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
BORIN,DIEGO B. MEZZOMO,NATHANA J. VAUCHER,RODRIGO A. CARMO,GUILHERME DO RODRIGUES JUNIOR,LUIZ C. SULCZEWSKI,FERNANDO B. SCHWERTZ,CLAITON I. MENDES,RICARDO E. DAMIANI,ADRIANI P. ANDRADE,VANESSA M. DE RECH,VIRGÍNIA C. BOECK,CARINA R. |
dc.subject.por.fl_str_mv |
nanocarrier neurodegenerative diseases nanotechnology liposomes |
topic |
nanocarrier neurodegenerative diseases nanotechnology liposomes |
description |
ABSTRACT Creatine acts intracellularly as energy buffer and storage, demonstrating protective effects in animal models of neurodegenerative diseases. However, its permeability throught blood-brain barrier (BBB) is reduced. The aim of the present study was developing a carrier to facilitate the delivery of creatine to the central nervous system. Creatine nanoliposomes were produced, characterized and assayed in models of toxicity in vitro and in vivo. Particles showed negative zeta potential (-12,5 mV), polydispersity index 0.237 and medium-size of 105 nm, which was confirmed by transmission electron microscopy (TEM) images. Toxicity assay in vitro was evaluated with blank liposomes (no drug) or creatine nanoliposomes at concentrations of 0.02 and 0.2 mg/mL, that did not influence the viability of Vero cells. The result. of the comet assay that the nanoliposomes are not genotoxic, togeher with cell viability demonstrated that the nanoliposomes are not toxic. Besides, in vivo assays not demonstrate toxicity in hematological and biochemical markers of young rats. Nevertheless, increase content of creatine in the cerebral cortex tissue after subchronic treatment was observed. Altogether, results indicate increase permeability of creatine to the BBB that could be used as assay for in vivo studies to confirm improved effect than free creatine. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502317 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502317 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0001-3765201820170553 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.90 n.2 suppl.1 2018 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
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1754302866539937792 |