FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae)

Detalhes bibliográficos
Autor(a) principal: AGUILERA,PATRICIA M.
Data de Publicação: 2016
Outros Autores: DEBAT,HUMBERTO J., SCALDAFERRO,MARISEL A., MARTÍ,DARDO A., GRABIELE,MAURO
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100117
Resumo: ABSTRACT We present here the physical mapping of the 5S rDNA locus in six wild and five cultivated taxa of Capsicum by means of a genus-specific FISH probe. In all taxa, a single 5S locus per haploid genome that persistently mapped onto the short arm of a unique metacentric chromosome pair at intercalar position, was found. 5S FISH signals of almost the same size and brightness intensity were observed in all the analyzed taxa. This is the first cytological characterization of the 5S in wild taxa of Capsicum by using a genus-derived probe, and the most exhaustive and comprehensive in the chili peppers up to now. The information provided here will aid the cytomolecular characterization of pepper germplasm to evaluate variability and can be instrumental to integrate physical, genetic and genomic maps already generated in the genus.
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spelling FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae)wild and cultivated Capsicum5S rRNA geneCapsicum-derived probeFISH-mappingABSTRACT We present here the physical mapping of the 5S rDNA locus in six wild and five cultivated taxa of Capsicum by means of a genus-specific FISH probe. In all taxa, a single 5S locus per haploid genome that persistently mapped onto the short arm of a unique metacentric chromosome pair at intercalar position, was found. 5S FISH signals of almost the same size and brightness intensity were observed in all the analyzed taxa. This is the first cytological characterization of the 5S in wild taxa of Capsicum by using a genus-derived probe, and the most exhaustive and comprehensive in the chili peppers up to now. The information provided here will aid the cytomolecular characterization of pepper germplasm to evaluate variability and can be instrumental to integrate physical, genetic and genomic maps already generated in the genus.Academia Brasileira de Ciências2016-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100117Anais da Academia Brasileira de Ciências v.88 n.1 2016reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-37652301620140616info:eu-repo/semantics/openAccessAGUILERA,PATRICIA M.DEBAT,HUMBERTO J.SCALDAFERRO,MARISEL A.MARTÍ,DARDO A.GRABIELE,MAUROeng2016-03-07T00:00:00Zoai:scielo:S0001-37652016000100117Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2016-03-07T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae)
title FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae)
spellingShingle FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae)
AGUILERA,PATRICIA M.
wild and cultivated Capsicum
5S rRNA gene
Capsicum-derived probe
FISH-mapping
title_short FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae)
title_full FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae)
title_fullStr FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae)
title_full_unstemmed FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae)
title_sort FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae)
author AGUILERA,PATRICIA M.
author_facet AGUILERA,PATRICIA M.
DEBAT,HUMBERTO J.
SCALDAFERRO,MARISEL A.
MARTÍ,DARDO A.
GRABIELE,MAURO
author_role author
author2 DEBAT,HUMBERTO J.
SCALDAFERRO,MARISEL A.
MARTÍ,DARDO A.
GRABIELE,MAURO
author2_role author
author
author
author
dc.contributor.author.fl_str_mv AGUILERA,PATRICIA M.
DEBAT,HUMBERTO J.
SCALDAFERRO,MARISEL A.
MARTÍ,DARDO A.
GRABIELE,MAURO
dc.subject.por.fl_str_mv wild and cultivated Capsicum
5S rRNA gene
Capsicum-derived probe
FISH-mapping
topic wild and cultivated Capsicum
5S rRNA gene
Capsicum-derived probe
FISH-mapping
description ABSTRACT We present here the physical mapping of the 5S rDNA locus in six wild and five cultivated taxa of Capsicum by means of a genus-specific FISH probe. In all taxa, a single 5S locus per haploid genome that persistently mapped onto the short arm of a unique metacentric chromosome pair at intercalar position, was found. 5S FISH signals of almost the same size and brightness intensity were observed in all the analyzed taxa. This is the first cytological characterization of the 5S in wild taxa of Capsicum by using a genus-derived probe, and the most exhaustive and comprehensive in the chili peppers up to now. The information provided here will aid the cytomolecular characterization of pepper germplasm to evaluate variability and can be instrumental to integrate physical, genetic and genomic maps already generated in the genus.
publishDate 2016
dc.date.none.fl_str_mv 2016-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100117
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100117
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-37652301620140616
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.88 n.1 2016
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
instacron_str ABC
institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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