Intestinal cell migration damage induced by enteropathogenic Escherichia coli strains
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000602 |
Resumo: | Epithelial cell migration is an essential response to enteric pathogens such as enteropathogenic Escherichia coli (EPEC). This study aimed to investigate the effects of EPEC infection on intestinal epithelial cell migration in vitro, as well as the involvement of type III secretion system (T3SS) and Rho GTPases. Crypt intestinal epithelial cells (IEC-6) were infected with EPEC strains (E2348/69, ΔescF, and the LDI001 strain isolated from a malnourished Brazilian child) and commensal E. coli HS. Wound migration and cell death assays were performed at different time-points. Transcription and expression of Rho GTPases were evaluated using real-time PCR and western blotting. Overall, EPEC E2348/69 reduced migration and increased apoptosis and necrosis levels compared to EPEC LDI001 and E. coli HS strains. Moreover, EPEC LDI001 impaired cell migration at a higher level than E. coli HS and increased necrosis after 24 hours compared to the control group. The different profiles of virulence genes between the two wild-type EPEC strains, characterized by the absence of espL and nleE genes in the LDI001, might explain the phenotypic results, playing significant roles on cell migration impairment and cell death-related events. Moreover, the type III secretion system is determinant for the inhibition of intestinal epithelial cell migration by EPEC 2348/69, as its deletion prevented the effect. Active Rac1 concentrations were increased in E2348/69 and LDI001-infected cells, while the T3SS-deficient strain did not demonstrate this activation. This study contributes with valuable insight to characterize the mechanisms involved in the impairment of intestinal cell migration induced by EPEC. |
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Brazilian Journal of Medical and Biological Research |
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Intestinal cell migration damage induced by enteropathogenic Escherichia coli strainsEnteropathogenic Escherichia coliIntestinal cell migrationType III secretion systemEpithelial cell migration is an essential response to enteric pathogens such as enteropathogenic Escherichia coli (EPEC). This study aimed to investigate the effects of EPEC infection on intestinal epithelial cell migration in vitro, as well as the involvement of type III secretion system (T3SS) and Rho GTPases. Crypt intestinal epithelial cells (IEC-6) were infected with EPEC strains (E2348/69, ΔescF, and the LDI001 strain isolated from a malnourished Brazilian child) and commensal E. coli HS. Wound migration and cell death assays were performed at different time-points. Transcription and expression of Rho GTPases were evaluated using real-time PCR and western blotting. Overall, EPEC E2348/69 reduced migration and increased apoptosis and necrosis levels compared to EPEC LDI001 and E. coli HS strains. Moreover, EPEC LDI001 impaired cell migration at a higher level than E. coli HS and increased necrosis after 24 hours compared to the control group. The different profiles of virulence genes between the two wild-type EPEC strains, characterized by the absence of espL and nleE genes in the LDI001, might explain the phenotypic results, playing significant roles on cell migration impairment and cell death-related events. Moreover, the type III secretion system is determinant for the inhibition of intestinal epithelial cell migration by EPEC 2348/69, as its deletion prevented the effect. Active Rac1 concentrations were increased in E2348/69 and LDI001-infected cells, while the T3SS-deficient strain did not demonstrate this activation. This study contributes with valuable insight to characterize the mechanisms involved in the impairment of intestinal cell migration induced by EPEC.Associação Brasileira de Divulgação Científica2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000602Brazilian Journal of Medical and Biological Research v.51 n.10 2018reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/1414-431x20187423info:eu-repo/semantics/openAccessCavalcante,P.A.Prata,M.M.G.Medeiros,P.H.Q.S.Alves da Silva,A.V.Quetz,J.S.Reyes,M.A.V.Rodrigues,T.S.Santos,A.K.S.Ribeiro,S.A.Veras,H.N.Bona,M.D.Amaral,M.S.M.G.Rodrigues,F.A.P.Lima,I.F.N.Havt,A.Lima,A.A.M.eng2019-03-19T00:00:00Zoai:scielo:S0100-879X2018001000602Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2019-03-19T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Intestinal cell migration damage induced by enteropathogenic Escherichia coli strains |
title |
Intestinal cell migration damage induced by enteropathogenic Escherichia coli strains |
spellingShingle |
Intestinal cell migration damage induced by enteropathogenic Escherichia coli strains Cavalcante,P.A. Enteropathogenic Escherichia coli Intestinal cell migration Type III secretion system |
title_short |
Intestinal cell migration damage induced by enteropathogenic Escherichia coli strains |
title_full |
Intestinal cell migration damage induced by enteropathogenic Escherichia coli strains |
title_fullStr |
Intestinal cell migration damage induced by enteropathogenic Escherichia coli strains |
title_full_unstemmed |
Intestinal cell migration damage induced by enteropathogenic Escherichia coli strains |
title_sort |
Intestinal cell migration damage induced by enteropathogenic Escherichia coli strains |
author |
Cavalcante,P.A. |
author_facet |
Cavalcante,P.A. Prata,M.M.G. Medeiros,P.H.Q.S. Alves da Silva,A.V. Quetz,J.S. Reyes,M.A.V. Rodrigues,T.S. Santos,A.K.S. Ribeiro,S.A. Veras,H.N. Bona,M.D. Amaral,M.S.M.G. Rodrigues,F.A.P. Lima,I.F.N. Havt,A. Lima,A.A.M. |
author_role |
author |
author2 |
Prata,M.M.G. Medeiros,P.H.Q.S. Alves da Silva,A.V. Quetz,J.S. Reyes,M.A.V. Rodrigues,T.S. Santos,A.K.S. Ribeiro,S.A. Veras,H.N. Bona,M.D. Amaral,M.S.M.G. Rodrigues,F.A.P. Lima,I.F.N. Havt,A. Lima,A.A.M. |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Cavalcante,P.A. Prata,M.M.G. Medeiros,P.H.Q.S. Alves da Silva,A.V. Quetz,J.S. Reyes,M.A.V. Rodrigues,T.S. Santos,A.K.S. Ribeiro,S.A. Veras,H.N. Bona,M.D. Amaral,M.S.M.G. Rodrigues,F.A.P. Lima,I.F.N. Havt,A. Lima,A.A.M. |
dc.subject.por.fl_str_mv |
Enteropathogenic Escherichia coli Intestinal cell migration Type III secretion system |
topic |
Enteropathogenic Escherichia coli Intestinal cell migration Type III secretion system |
description |
Epithelial cell migration is an essential response to enteric pathogens such as enteropathogenic Escherichia coli (EPEC). This study aimed to investigate the effects of EPEC infection on intestinal epithelial cell migration in vitro, as well as the involvement of type III secretion system (T3SS) and Rho GTPases. Crypt intestinal epithelial cells (IEC-6) were infected with EPEC strains (E2348/69, ΔescF, and the LDI001 strain isolated from a malnourished Brazilian child) and commensal E. coli HS. Wound migration and cell death assays were performed at different time-points. Transcription and expression of Rho GTPases were evaluated using real-time PCR and western blotting. Overall, EPEC E2348/69 reduced migration and increased apoptosis and necrosis levels compared to EPEC LDI001 and E. coli HS strains. Moreover, EPEC LDI001 impaired cell migration at a higher level than E. coli HS and increased necrosis after 24 hours compared to the control group. The different profiles of virulence genes between the two wild-type EPEC strains, characterized by the absence of espL and nleE genes in the LDI001, might explain the phenotypic results, playing significant roles on cell migration impairment and cell death-related events. Moreover, the type III secretion system is determinant for the inhibition of intestinal epithelial cell migration by EPEC 2348/69, as its deletion prevented the effect. Active Rac1 concentrations were increased in E2348/69 and LDI001-infected cells, while the T3SS-deficient strain did not demonstrate this activation. This study contributes with valuable insight to characterize the mechanisms involved in the impairment of intestinal cell migration induced by EPEC. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000602 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000602 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1414-431x20187423 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.51 n.10 2018 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302946678407168 |