Detection and genetic analysis of the enteroaggregative Escherichia coli heat-stable enterotoxin (EAST1) gene in clinical isolates of enteropathogenic Escherichia coli (EPEC) strains
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/37788 http://dx.doi.org/10.1186/1471-2180-14-135 |
Resumo: | Background: the enteroaggregative E. coli heat-stable enterotoxin 1 (EAST1) encoded by astA gene has been found in enteropathogenic E. coli (EPEC) strains. However, it is not sufficient to simply probe strains with an astA gene probe due to the existence of astA mutants (type 1 and type 2 SHEAST) and EAST1 variants (EAST1 v1-4). in this study, 222 EPEC (70 typical and 152 atypical) isolates were tested for the presence of the astA gene sequence by PCR and sequencing.Results: the astA gene was amplified from 54 strains, 11 typical and 43 atypical. Sequence analysis of the PCR products showed that 25 strains, 7 typical and 18 atypical, had an intact astA gene. A subgroup of 7 atypical strains had a variant type of the astA gene sequence, with four non-synonymous nucleotide substitutions. the remaining 22 strains had mutated astA gene with nucleotide deletions or substitutions in the first 8 codons. the RT-PCR results showed that the astA gene was transcribed only by the strains carrying either the intact or the variant type of the astA gene sequence. Southern blot analysis indicated that astA is located in EAF plasmid in typical strains, and in plasmids of similar size in atypical strains. Strains carrying intact astA genes were more frequently found in diarrheic children than in non-diarrheic children (p < 0.05).Conclusion: in conclusion, our data suggest that the presence of an intact astA gene may represent an additional virulence determinant in both EPEC groups. |
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Silva, Lucas E. P. [UNIFESP]Souza, Tamara B. [UNIFESP]Silva, Neusa Pereira da [UNIFESP]Scaletsky, Isabel Cristina Affonso [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:37:19Z2016-01-24T14:37:19Z2014-05-30Bmc Microbiology. London: Biomed Central Ltd, v. 14, 6 p., 2014.1471-2180http://repositorio.unifesp.br/handle/11600/37788http://dx.doi.org/10.1186/1471-2180-14-135WOS000338157100002.pdf10.1186/1471-2180-14-135WOS:000338157100002Background: the enteroaggregative E. coli heat-stable enterotoxin 1 (EAST1) encoded by astA gene has been found in enteropathogenic E. coli (EPEC) strains. However, it is not sufficient to simply probe strains with an astA gene probe due to the existence of astA mutants (type 1 and type 2 SHEAST) and EAST1 variants (EAST1 v1-4). in this study, 222 EPEC (70 typical and 152 atypical) isolates were tested for the presence of the astA gene sequence by PCR and sequencing.Results: the astA gene was amplified from 54 strains, 11 typical and 43 atypical. Sequence analysis of the PCR products showed that 25 strains, 7 typical and 18 atypical, had an intact astA gene. A subgroup of 7 atypical strains had a variant type of the astA gene sequence, with four non-synonymous nucleotide substitutions. the remaining 22 strains had mutated astA gene with nucleotide deletions or substitutions in the first 8 codons. the RT-PCR results showed that the astA gene was transcribed only by the strains carrying either the intact or the variant type of the astA gene sequence. Southern blot analysis indicated that astA is located in EAF plasmid in typical strains, and in plasmids of similar size in atypical strains. Strains carrying intact astA genes were more frequently found in diarrheic children than in non-diarrheic children (p < 0.05).Conclusion: in conclusion, our data suggest that the presence of an intact astA gene may represent an additional virulence determinant in both EPEC groups.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Disciplina Reumatol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Disciplina Reumatol, São Paulo, BrazilWeb of Science6engBiomed Central LtdBmc MicrobiologyEAST1 geneastA geneEnteropathogenic Escherichia coliDetection and genetic analysis of the enteroaggregative Escherichia coli heat-stable enterotoxin (EAST1) gene in clinical isolates of enteropathogenic Escherichia coli (EPEC) strainsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000338157100002.pdfapplication/pdf799659${dspace.ui.url}/bitstream/11600/37788/1/WOS000338157100002.pdffa10f90a970708191ffbbac1da561faaMD51open accessTEXTWOS000338157100002.pdf.txtWOS000338157100002.pdf.txtExtracted texttext/plain30340${dspace.ui.url}/bitstream/11600/37788/2/WOS000338157100002.pdf.txt960ef204ae55450a1c8e676329f050ffMD52open access11600/377882022-07-08 10:33:31.379open accessoai:repositorio.unifesp.br:11600/37788Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:19:00.637616Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Detection and genetic analysis of the enteroaggregative Escherichia coli heat-stable enterotoxin (EAST1) gene in clinical isolates of enteropathogenic Escherichia coli (EPEC) strains |
title |
Detection and genetic analysis of the enteroaggregative Escherichia coli heat-stable enterotoxin (EAST1) gene in clinical isolates of enteropathogenic Escherichia coli (EPEC) strains |
spellingShingle |
Detection and genetic analysis of the enteroaggregative Escherichia coli heat-stable enterotoxin (EAST1) gene in clinical isolates of enteropathogenic Escherichia coli (EPEC) strains Silva, Lucas E. P. [UNIFESP] EAST1 gene astA gene Enteropathogenic Escherichia coli |
title_short |
Detection and genetic analysis of the enteroaggregative Escherichia coli heat-stable enterotoxin (EAST1) gene in clinical isolates of enteropathogenic Escherichia coli (EPEC) strains |
title_full |
Detection and genetic analysis of the enteroaggregative Escherichia coli heat-stable enterotoxin (EAST1) gene in clinical isolates of enteropathogenic Escherichia coli (EPEC) strains |
title_fullStr |
Detection and genetic analysis of the enteroaggregative Escherichia coli heat-stable enterotoxin (EAST1) gene in clinical isolates of enteropathogenic Escherichia coli (EPEC) strains |
title_full_unstemmed |
Detection and genetic analysis of the enteroaggregative Escherichia coli heat-stable enterotoxin (EAST1) gene in clinical isolates of enteropathogenic Escherichia coli (EPEC) strains |
title_sort |
Detection and genetic analysis of the enteroaggregative Escherichia coli heat-stable enterotoxin (EAST1) gene in clinical isolates of enteropathogenic Escherichia coli (EPEC) strains |
author |
Silva, Lucas E. P. [UNIFESP] |
author_facet |
Silva, Lucas E. P. [UNIFESP] Souza, Tamara B. [UNIFESP] Silva, Neusa Pereira da [UNIFESP] Scaletsky, Isabel Cristina Affonso [UNIFESP] |
author_role |
author |
author2 |
Souza, Tamara B. [UNIFESP] Silva, Neusa Pereira da [UNIFESP] Scaletsky, Isabel Cristina Affonso [UNIFESP] |
author2_role |
author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Silva, Lucas E. P. [UNIFESP] Souza, Tamara B. [UNIFESP] Silva, Neusa Pereira da [UNIFESP] Scaletsky, Isabel Cristina Affonso [UNIFESP] |
dc.subject.eng.fl_str_mv |
EAST1 gene astA gene Enteropathogenic Escherichia coli |
topic |
EAST1 gene astA gene Enteropathogenic Escherichia coli |
description |
Background: the enteroaggregative E. coli heat-stable enterotoxin 1 (EAST1) encoded by astA gene has been found in enteropathogenic E. coli (EPEC) strains. However, it is not sufficient to simply probe strains with an astA gene probe due to the existence of astA mutants (type 1 and type 2 SHEAST) and EAST1 variants (EAST1 v1-4). in this study, 222 EPEC (70 typical and 152 atypical) isolates were tested for the presence of the astA gene sequence by PCR and sequencing.Results: the astA gene was amplified from 54 strains, 11 typical and 43 atypical. Sequence analysis of the PCR products showed that 25 strains, 7 typical and 18 atypical, had an intact astA gene. A subgroup of 7 atypical strains had a variant type of the astA gene sequence, with four non-synonymous nucleotide substitutions. the remaining 22 strains had mutated astA gene with nucleotide deletions or substitutions in the first 8 codons. the RT-PCR results showed that the astA gene was transcribed only by the strains carrying either the intact or the variant type of the astA gene sequence. Southern blot analysis indicated that astA is located in EAF plasmid in typical strains, and in plasmids of similar size in atypical strains. Strains carrying intact astA genes were more frequently found in diarrheic children than in non-diarrheic children (p < 0.05).Conclusion: in conclusion, our data suggest that the presence of an intact astA gene may represent an additional virulence determinant in both EPEC groups. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-05-30 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:37:19Z |
dc.date.available.fl_str_mv |
2016-01-24T14:37:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Bmc Microbiology. London: Biomed Central Ltd, v. 14, 6 p., 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/37788 http://dx.doi.org/10.1186/1471-2180-14-135 |
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1471-2180 |
dc.identifier.file.none.fl_str_mv |
WOS000338157100002.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1186/1471-2180-14-135 |
dc.identifier.wos.none.fl_str_mv |
WOS:000338157100002 |
identifier_str_mv |
Bmc Microbiology. London: Biomed Central Ltd, v. 14, 6 p., 2014. 1471-2180 WOS000338157100002.pdf 10.1186/1471-2180-14-135 WOS:000338157100002 |
url |
http://repositorio.unifesp.br/handle/11600/37788 http://dx.doi.org/10.1186/1471-2180-14-135 |
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eng |
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Biomed Central Ltd |
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Biomed Central Ltd |
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