Extracellular ATP in the lymphohematopoietic system: P2Z purinoceptors and membrane permeabilization
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100004 |
Resumo: | The effects of extracellular nucleosides and nucleotides on many organs and systems have been recognized for almost 50 years. The effects of extracellular ATP (ATPo), UTPo, ADPo, and other agonists are mediated by P2 purinoceptors. One of the most dramatic effects of ATPo is the permeabilization of plasma membranes to low molecular mass solutes of up to 900 Da. This effect is evident in several cells of the lymphohematopoietic system and is supposed to be mediated by P2Z, an ATP4--activated purinoceptor. Here, we review some basic information concerning P2 purinoceptors and focus our attention on P2Z-associated phenomena displayed by macrophages. Using fluorescent dye uptake, measurement of free intracellular Ca2+ concentration and electrophysiological recordings, we elucidate some of the events that follow the application of ATP to the extracellular surface of macrophages. We propose a regulatory mechanism for the P2Z-associated permeabilization pore. The presence of P2 purinoceptors in cells of the lymphohematopoietic system makes them potential candidates to mediate immunoregulatory events |
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Brazilian Journal of Medical and Biological Research |
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Extracellular ATP in the lymphohematopoietic system: P2Z purinoceptors and membrane permeabilizationATPP2Z purinoceptormacrophagemonocyteT cellpermeabilizationThe effects of extracellular nucleosides and nucleotides on many organs and systems have been recognized for almost 50 years. The effects of extracellular ATP (ATPo), UTPo, ADPo, and other agonists are mediated by P2 purinoceptors. One of the most dramatic effects of ATPo is the permeabilization of plasma membranes to low molecular mass solutes of up to 900 Da. This effect is evident in several cells of the lymphohematopoietic system and is supposed to be mediated by P2Z, an ATP4--activated purinoceptor. Here, we review some basic information concerning P2 purinoceptors and focus our attention on P2Z-associated phenomena displayed by macrophages. Using fluorescent dye uptake, measurement of free intracellular Ca2+ concentration and electrophysiological recordings, we elucidate some of the events that follow the application of ATP to the extracellular surface of macrophages. We propose a regulatory mechanism for the P2Z-associated permeabilization pore. The presence of P2 purinoceptors in cells of the lymphohematopoietic system makes them potential candidates to mediate immunoregulatory eventsAssociação Brasileira de Divulgação Científica1998-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100004Brazilian Journal of Medical and Biological Research v.31 n.1 1998reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X1998000100004info:eu-repo/semantics/openAccessPersechini,P.M.Bisaggio,R.C.Alves-Neto,J.L.Coutinho-Silva,R.eng1998-10-07T00:00:00Zoai:scielo:S0100-879X1998000100004Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:1998-10-07T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Extracellular ATP in the lymphohematopoietic system: P2Z purinoceptors and membrane permeabilization |
title |
Extracellular ATP in the lymphohematopoietic system: P2Z purinoceptors and membrane permeabilization |
spellingShingle |
Extracellular ATP in the lymphohematopoietic system: P2Z purinoceptors and membrane permeabilization Persechini,P.M. ATP P2Z purinoceptor macrophage monocyte T cell permeabilization |
title_short |
Extracellular ATP in the lymphohematopoietic system: P2Z purinoceptors and membrane permeabilization |
title_full |
Extracellular ATP in the lymphohematopoietic system: P2Z purinoceptors and membrane permeabilization |
title_fullStr |
Extracellular ATP in the lymphohematopoietic system: P2Z purinoceptors and membrane permeabilization |
title_full_unstemmed |
Extracellular ATP in the lymphohematopoietic system: P2Z purinoceptors and membrane permeabilization |
title_sort |
Extracellular ATP in the lymphohematopoietic system: P2Z purinoceptors and membrane permeabilization |
author |
Persechini,P.M. |
author_facet |
Persechini,P.M. Bisaggio,R.C. Alves-Neto,J.L. Coutinho-Silva,R. |
author_role |
author |
author2 |
Bisaggio,R.C. Alves-Neto,J.L. Coutinho-Silva,R. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Persechini,P.M. Bisaggio,R.C. Alves-Neto,J.L. Coutinho-Silva,R. |
dc.subject.por.fl_str_mv |
ATP P2Z purinoceptor macrophage monocyte T cell permeabilization |
topic |
ATP P2Z purinoceptor macrophage monocyte T cell permeabilization |
description |
The effects of extracellular nucleosides and nucleotides on many organs and systems have been recognized for almost 50 years. The effects of extracellular ATP (ATPo), UTPo, ADPo, and other agonists are mediated by P2 purinoceptors. One of the most dramatic effects of ATPo is the permeabilization of plasma membranes to low molecular mass solutes of up to 900 Da. This effect is evident in several cells of the lymphohematopoietic system and is supposed to be mediated by P2Z, an ATP4--activated purinoceptor. Here, we review some basic information concerning P2 purinoceptors and focus our attention on P2Z-associated phenomena displayed by macrophages. Using fluorescent dye uptake, measurement of free intracellular Ca2+ concentration and electrophysiological recordings, we elucidate some of the events that follow the application of ATP to the extracellular surface of macrophages. We propose a regulatory mechanism for the P2Z-associated permeabilization pore. The presence of P2 purinoceptors in cells of the lymphohematopoietic system makes them potential candidates to mediate immunoregulatory events |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-879X1998000100004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.31 n.1 1998 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302928906092544 |