Lipid peroxidation and total radical-trapping potential of the lungs of rats submitted to chronic and sub-chronic stress
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200004 |
Resumo: | Exposure to stress induces a cluster of physiological and behavioral changes in an effort to maintain the homeostasis of the organism. Long-term exposure to stress, however, has detrimental effects on several cell functions such as the impairment of antioxidant defenses leading to oxidative damage. Oxidative stress is a central feature of many diseases. The lungs are particularly susceptible to lesions by free radicals and pulmonary antioxidant defenses are extensively distributed and include both enzymatic and non-enzymatic systems. The aim of the present study was to determine lipid peroxidation and total radical-trapping potential (TRAP) changes in lungs of rats submitted to different models of chronic stress. Adult male Wistar rats weighing 180-230 g were submitted to different stressors (variable stress, N = 7) or repeated restraint stress for 15 (N = 10) or 40 days (N = 6) and compared to control groups (N = 10 each). Lipid peroxidation levels were assessed by thiobarbituric acid reactive substances (TBARS), and TRAP was measured by the decrease in luminescence using the 2-2'-azo-bis(2-amidinopropane)-luminol system. Chronic variable stress induced a 51% increase in oxidative stress in lungs (control group: 0.037 ± 0.002; variable stress: 0.056 ± 0.007, P < 0.01). No difference in TBARS was observed after chronic restraint stress, but a significant 57% increase in TRAP was presented by the group repeatedly restrained for 15 days (control group: 2.48 ± 0.42; stressed: 3.65 ± 0.16, P < 0.05). We conclude that different stressors induce different effects on the oxidative status of the organism. |
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Brazilian Journal of Medical and Biological Research |
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Lipid peroxidation and total radical-trapping potential of the lungs of rats submitted to chronic and sub-chronic stressStressTBARSTRAPFree radicalsLungsOxidative stressExposure to stress induces a cluster of physiological and behavioral changes in an effort to maintain the homeostasis of the organism. Long-term exposure to stress, however, has detrimental effects on several cell functions such as the impairment of antioxidant defenses leading to oxidative damage. Oxidative stress is a central feature of many diseases. The lungs are particularly susceptible to lesions by free radicals and pulmonary antioxidant defenses are extensively distributed and include both enzymatic and non-enzymatic systems. The aim of the present study was to determine lipid peroxidation and total radical-trapping potential (TRAP) changes in lungs of rats submitted to different models of chronic stress. Adult male Wistar rats weighing 180-230 g were submitted to different stressors (variable stress, N = 7) or repeated restraint stress for 15 (N = 10) or 40 days (N = 6) and compared to control groups (N = 10 each). Lipid peroxidation levels were assessed by thiobarbituric acid reactive substances (TBARS), and TRAP was measured by the decrease in luminescence using the 2-2'-azo-bis(2-amidinopropane)-luminol system. Chronic variable stress induced a 51% increase in oxidative stress in lungs (control group: 0.037 ± 0.002; variable stress: 0.056 ± 0.007, P < 0.01). No difference in TBARS was observed after chronic restraint stress, but a significant 57% increase in TRAP was presented by the group repeatedly restrained for 15 days (control group: 2.48 ± 0.42; stressed: 3.65 ± 0.16, P < 0.05). We conclude that different stressors induce different effects on the oxidative status of the organism.Associação Brasileira de Divulgação Científica2004-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200004Brazilian Journal of Medical and Biological Research v.37 n.2 2004reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2004000200004info:eu-repo/semantics/openAccessTorres,R.L.Torres,I.L.S.Gamaro,G.D.Fontella,F.U.Silveira,P.P.Moreira,J.S.R.Lacerda,M.Amoretti,J.R.Rech,D.Dalmaz,C.Belló,A.A.eng2004-01-30T00:00:00Zoai:scielo:S0100-879X2004000200004Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2004-01-30T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Lipid peroxidation and total radical-trapping potential of the lungs of rats submitted to chronic and sub-chronic stress |
title |
Lipid peroxidation and total radical-trapping potential of the lungs of rats submitted to chronic and sub-chronic stress |
spellingShingle |
Lipid peroxidation and total radical-trapping potential of the lungs of rats submitted to chronic and sub-chronic stress Torres,R.L. Stress TBARS TRAP Free radicals Lungs Oxidative stress |
title_short |
Lipid peroxidation and total radical-trapping potential of the lungs of rats submitted to chronic and sub-chronic stress |
title_full |
Lipid peroxidation and total radical-trapping potential of the lungs of rats submitted to chronic and sub-chronic stress |
title_fullStr |
Lipid peroxidation and total radical-trapping potential of the lungs of rats submitted to chronic and sub-chronic stress |
title_full_unstemmed |
Lipid peroxidation and total radical-trapping potential of the lungs of rats submitted to chronic and sub-chronic stress |
title_sort |
Lipid peroxidation and total radical-trapping potential of the lungs of rats submitted to chronic and sub-chronic stress |
author |
Torres,R.L. |
author_facet |
Torres,R.L. Torres,I.L.S. Gamaro,G.D. Fontella,F.U. Silveira,P.P. Moreira,J.S.R. Lacerda,M. Amoretti,J.R. Rech,D. Dalmaz,C. Belló,A.A. |
author_role |
author |
author2 |
Torres,I.L.S. Gamaro,G.D. Fontella,F.U. Silveira,P.P. Moreira,J.S.R. Lacerda,M. Amoretti,J.R. Rech,D. Dalmaz,C. Belló,A.A. |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Torres,R.L. Torres,I.L.S. Gamaro,G.D. Fontella,F.U. Silveira,P.P. Moreira,J.S.R. Lacerda,M. Amoretti,J.R. Rech,D. Dalmaz,C. Belló,A.A. |
dc.subject.por.fl_str_mv |
Stress TBARS TRAP Free radicals Lungs Oxidative stress |
topic |
Stress TBARS TRAP Free radicals Lungs Oxidative stress |
description |
Exposure to stress induces a cluster of physiological and behavioral changes in an effort to maintain the homeostasis of the organism. Long-term exposure to stress, however, has detrimental effects on several cell functions such as the impairment of antioxidant defenses leading to oxidative damage. Oxidative stress is a central feature of many diseases. The lungs are particularly susceptible to lesions by free radicals and pulmonary antioxidant defenses are extensively distributed and include both enzymatic and non-enzymatic systems. The aim of the present study was to determine lipid peroxidation and total radical-trapping potential (TRAP) changes in lungs of rats submitted to different models of chronic stress. Adult male Wistar rats weighing 180-230 g were submitted to different stressors (variable stress, N = 7) or repeated restraint stress for 15 (N = 10) or 40 days (N = 6) and compared to control groups (N = 10 each). Lipid peroxidation levels were assessed by thiobarbituric acid reactive substances (TBARS), and TRAP was measured by the decrease in luminescence using the 2-2'-azo-bis(2-amidinopropane)-luminol system. Chronic variable stress induced a 51% increase in oxidative stress in lungs (control group: 0.037 ± 0.002; variable stress: 0.056 ± 0.007, P < 0.01). No difference in TBARS was observed after chronic restraint stress, but a significant 57% increase in TRAP was presented by the group repeatedly restrained for 15 days (control group: 2.48 ± 0.42; stressed: 3.65 ± 0.16, P < 0.05). We conclude that different stressors induce different effects on the oxidative status of the organism. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-879X2004000200004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.37 n.2 2004 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
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1754302932915847168 |