Antiproliferative and photoprotective activities of the extracts and compounds from Calea fruticosa

• Autor(a) principal: Seregheti,T.M.Q.
• Data de Publicação: 2020
• Outros Autores: Pinto,A.P.R., Gonçalves,M.da C., Antunes,A.dos S., Almeida,W.A.da S., Machado,R.S., Silva,J.N., Ferreira,P.M.P., Pessoa,C., Santos,V.M.R. dos, Nascimento,A.M. do
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Medical and Biological Research
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000900601
• topic: Flavone; Sesquiterpenic lactone; Flavonol; Glucosylated coumarin; Sunscreen preparation

In this paper, we complement our previous study on the antiproliferative activity of Calea fruticosa (Asteraceae) by isolating the compounds apigenin-4',7-dimethyl ether (1), budlein A (2), quercetin (3), and cichoriin (4) from the plant’s aerial parts. The antiproliferative activity of these compounds was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method against human tumor cell lines. Compound 3 displayed moderate antiproliferative activity in three cell lines (HCT-116, PC-3, and SF-295, with cell growth inhibition values of 72.97, 74.55, and 68.94%) and high antiproliferative activity (90.86%) in the HL-60 cell line. The in vitro sun protection factor (SPF) of the extracts and compound 4, with and without sunscreen, was determined by a spectrophotometric method. The ethanol extract exhibited the highest SPF (9.67) at a concentration of 0.100 mg/mL, while compound 4, isolated from this extract, showed a SPF of 13.79 at the same concentration. A relative increased efficacy of SPF was observed for the extracts and compound 4 when sunscreen was also used. Compound 4 has not been reported previously from any species within the genus Calea. Compounds 1–4 were obtained from this species for the first time.