Mechanisms involved in anti-aging effects of guarana (Paullinia cupana) in Caenorhabditis elegans
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900602 |
Resumo: | Guarana (Paullinia cupana) is habitually ingested by people in the Amazon region and is a key ingredient in various energy drinks consumed worldwide. Extension in longevity and low prevalence of chronic age-related diseases have been associated to habitual intake of guarana. Anti-aging potential of guarana was also demonstrated in Caenorhabditis elegans; however, the mechanisms involved in its effects are not clear. Herein, we investigated the putative pathways that regulate the effects of guarana ethanolic extract (GEE) on lifespan using C. elegans. The major known longevity pathways were analyzed through mutant worms and RT-qPCR assay (DAF-2, DAF-16, SKN-1, SIR-2.1, HSF-1). The possible involvement of purinergic signaling was also investigated. This study demonstrated that GEE acts through antioxidant activity, DAF-16, HSF-1, and SKN-1 pathways, and human adenosine receptor ortholog (ADOR-1) to extend lifespan. GEE also downregulated skn-1, daf-16, sir-2.1 and hsp-16.2 in 9-day-old C. elegans, which might reflect less need to activate these protective genes due to direct antioxidant effects. Our results contribute to the comprehension of guarana effects in vivo, which might be helpful to prevent or treat aging-associated disorders, and also suggest purinergic signaling as a plausible therapeutic target for longevity studies. |
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Brazilian Journal of Medical and Biological Research |
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Mechanisms involved in anti-aging effects of guarana (Paullinia cupana) in Caenorhabditis elegansAgingAntioxidantGuaranaLifespanNatural productsXanthinesGuarana (Paullinia cupana) is habitually ingested by people in the Amazon region and is a key ingredient in various energy drinks consumed worldwide. Extension in longevity and low prevalence of chronic age-related diseases have been associated to habitual intake of guarana. Anti-aging potential of guarana was also demonstrated in Caenorhabditis elegans; however, the mechanisms involved in its effects are not clear. Herein, we investigated the putative pathways that regulate the effects of guarana ethanolic extract (GEE) on lifespan using C. elegans. The major known longevity pathways were analyzed through mutant worms and RT-qPCR assay (DAF-2, DAF-16, SKN-1, SIR-2.1, HSF-1). The possible involvement of purinergic signaling was also investigated. This study demonstrated that GEE acts through antioxidant activity, DAF-16, HSF-1, and SKN-1 pathways, and human adenosine receptor ortholog (ADOR-1) to extend lifespan. GEE also downregulated skn-1, daf-16, sir-2.1 and hsp-16.2 in 9-day-old C. elegans, which might reflect less need to activate these protective genes due to direct antioxidant effects. Our results contribute to the comprehension of guarana effects in vivo, which might be helpful to prevent or treat aging-associated disorders, and also suggest purinergic signaling as a plausible therapeutic target for longevity studies.Associação Brasileira de Divulgação Científica2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900602Brazilian Journal of Medical and Biological Research v.51 n.9 2018reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/1414-431x20187552info:eu-repo/semantics/openAccessArantes,L.P.Machado,M.L.Zamberlan,D.C.da Silveira,T.L.da Silva,T.C.da Cruz,I.B.M.Ribeiro,E.E.Aschner,M.Soares,F.A.A.eng2019-03-19T00:00:00Zoai:scielo:S0100-879X2018000900602Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2019-03-19T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Mechanisms involved in anti-aging effects of guarana (Paullinia cupana) in Caenorhabditis elegans |
title |
Mechanisms involved in anti-aging effects of guarana (Paullinia cupana) in Caenorhabditis elegans |
spellingShingle |
Mechanisms involved in anti-aging effects of guarana (Paullinia cupana) in Caenorhabditis elegans Arantes,L.P. Aging Antioxidant Guarana Lifespan Natural products Xanthines |
title_short |
Mechanisms involved in anti-aging effects of guarana (Paullinia cupana) in Caenorhabditis elegans |
title_full |
Mechanisms involved in anti-aging effects of guarana (Paullinia cupana) in Caenorhabditis elegans |
title_fullStr |
Mechanisms involved in anti-aging effects of guarana (Paullinia cupana) in Caenorhabditis elegans |
title_full_unstemmed |
Mechanisms involved in anti-aging effects of guarana (Paullinia cupana) in Caenorhabditis elegans |
title_sort |
Mechanisms involved in anti-aging effects of guarana (Paullinia cupana) in Caenorhabditis elegans |
author |
Arantes,L.P. |
author_facet |
Arantes,L.P. Machado,M.L. Zamberlan,D.C. da Silveira,T.L. da Silva,T.C. da Cruz,I.B.M. Ribeiro,E.E. Aschner,M. Soares,F.A.A. |
author_role |
author |
author2 |
Machado,M.L. Zamberlan,D.C. da Silveira,T.L. da Silva,T.C. da Cruz,I.B.M. Ribeiro,E.E. Aschner,M. Soares,F.A.A. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Arantes,L.P. Machado,M.L. Zamberlan,D.C. da Silveira,T.L. da Silva,T.C. da Cruz,I.B.M. Ribeiro,E.E. Aschner,M. Soares,F.A.A. |
dc.subject.por.fl_str_mv |
Aging Antioxidant Guarana Lifespan Natural products Xanthines |
topic |
Aging Antioxidant Guarana Lifespan Natural products Xanthines |
description |
Guarana (Paullinia cupana) is habitually ingested by people in the Amazon region and is a key ingredient in various energy drinks consumed worldwide. Extension in longevity and low prevalence of chronic age-related diseases have been associated to habitual intake of guarana. Anti-aging potential of guarana was also demonstrated in Caenorhabditis elegans; however, the mechanisms involved in its effects are not clear. Herein, we investigated the putative pathways that regulate the effects of guarana ethanolic extract (GEE) on lifespan using C. elegans. The major known longevity pathways were analyzed through mutant worms and RT-qPCR assay (DAF-2, DAF-16, SKN-1, SIR-2.1, HSF-1). The possible involvement of purinergic signaling was also investigated. This study demonstrated that GEE acts through antioxidant activity, DAF-16, HSF-1, and SKN-1 pathways, and human adenosine receptor ortholog (ADOR-1) to extend lifespan. GEE also downregulated skn-1, daf-16, sir-2.1 and hsp-16.2 in 9-day-old C. elegans, which might reflect less need to activate these protective genes due to direct antioxidant effects. Our results contribute to the comprehension of guarana effects in vivo, which might be helpful to prevent or treat aging-associated disorders, and also suggest purinergic signaling as a plausible therapeutic target for longevity studies. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900602 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900602 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1414-431x20187552 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.51 n.9 2018 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
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1754302946653241344 |