Identification and characterization of the two-component NtrY/NtrX regulatory system in Azospirillum brasilense

Detalhes bibliográficos
Autor(a) principal: Ishida,M.L.
Data de Publicação: 2002
Outros Autores: Assumpção,M.C., Machado,H.B., Benelli,E.M., Souza,E.M., Pedrosa,F.O.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600004
Resumo: Two Azospirillum brasilense open reading frames (ORFs) exhibited homology with the two-component NtrY/NtrX regulatory system from Azorhizobium caulinodans. These A. brasilense ORFs, located downstream to the nifR3ntrBC operon, were isolated, sequenced and characterized. The present study suggests that ORF1 and ORF2 correspond to the A. brasilense ntrY and ntrX genes, respectively. The amino acid sequences of A. brasilense NtrY and NtrX proteins showed high similarity to sensor/kinase and regulatory proteins, respectively. Analysis of lacZ transcriptional fusions by the ß-galactosidase assay in Escherichia coli ntrC mutants showed that the NtrY/NtrX proteins failed to activate transcription of the nifA promoter of A. brasilense. The ntrYX operon complemented a nifR3ntrBC deletion mutant of A. brasilense for nitrate-dependent growth, suggesting a possible cross-talk between the NtrY/X and NtrB/C sensor/regulator pairs. Our data support the existence of another two-component regulatory system in A. brasilense, the NtrY/NtrX system, probably involved in the regulation of nitrate assimilation.
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spelling Identification and characterization of the two-component NtrY/NtrX regulatory system in Azospirillum brasilenseAzospirillum brasilenseTwo-component regulatory systemNitrogen regulationntrYX genesntrBC genesTwo Azospirillum brasilense open reading frames (ORFs) exhibited homology with the two-component NtrY/NtrX regulatory system from Azorhizobium caulinodans. These A. brasilense ORFs, located downstream to the nifR3ntrBC operon, were isolated, sequenced and characterized. The present study suggests that ORF1 and ORF2 correspond to the A. brasilense ntrY and ntrX genes, respectively. The amino acid sequences of A. brasilense NtrY and NtrX proteins showed high similarity to sensor/kinase and regulatory proteins, respectively. Analysis of lacZ transcriptional fusions by the ß-galactosidase assay in Escherichia coli ntrC mutants showed that the NtrY/NtrX proteins failed to activate transcription of the nifA promoter of A. brasilense. The ntrYX operon complemented a nifR3ntrBC deletion mutant of A. brasilense for nitrate-dependent growth, suggesting a possible cross-talk between the NtrY/X and NtrB/C sensor/regulator pairs. Our data support the existence of another two-component regulatory system in A. brasilense, the NtrY/NtrX system, probably involved in the regulation of nitrate assimilation.Associação Brasileira de Divulgação Científica2002-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600004Brazilian Journal of Medical and Biological Research v.35 n.6 2002reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2002000600004info:eu-repo/semantics/openAccessIshida,M.L.Assumpção,M.C.Machado,H.B.Benelli,E.M.Souza,E.M.Pedrosa,F.O.eng2002-05-28T00:00:00Zoai:scielo:S0100-879X2002000600004Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2002-05-28T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Identification and characterization of the two-component NtrY/NtrX regulatory system in Azospirillum brasilense
title Identification and characterization of the two-component NtrY/NtrX regulatory system in Azospirillum brasilense
spellingShingle Identification and characterization of the two-component NtrY/NtrX regulatory system in Azospirillum brasilense
Ishida,M.L.
Azospirillum brasilense
Two-component regulatory system
Nitrogen regulation
ntrYX genes
ntrBC genes
title_short Identification and characterization of the two-component NtrY/NtrX regulatory system in Azospirillum brasilense
title_full Identification and characterization of the two-component NtrY/NtrX regulatory system in Azospirillum brasilense
title_fullStr Identification and characterization of the two-component NtrY/NtrX regulatory system in Azospirillum brasilense
title_full_unstemmed Identification and characterization of the two-component NtrY/NtrX regulatory system in Azospirillum brasilense
title_sort Identification and characterization of the two-component NtrY/NtrX regulatory system in Azospirillum brasilense
author Ishida,M.L.
author_facet Ishida,M.L.
Assumpção,M.C.
Machado,H.B.
Benelli,E.M.
Souza,E.M.
Pedrosa,F.O.
author_role author
author2 Assumpção,M.C.
Machado,H.B.
Benelli,E.M.
Souza,E.M.
Pedrosa,F.O.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Ishida,M.L.
Assumpção,M.C.
Machado,H.B.
Benelli,E.M.
Souza,E.M.
Pedrosa,F.O.
dc.subject.por.fl_str_mv Azospirillum brasilense
Two-component regulatory system
Nitrogen regulation
ntrYX genes
ntrBC genes
topic Azospirillum brasilense
Two-component regulatory system
Nitrogen regulation
ntrYX genes
ntrBC genes
description Two Azospirillum brasilense open reading frames (ORFs) exhibited homology with the two-component NtrY/NtrX regulatory system from Azorhizobium caulinodans. These A. brasilense ORFs, located downstream to the nifR3ntrBC operon, were isolated, sequenced and characterized. The present study suggests that ORF1 and ORF2 correspond to the A. brasilense ntrY and ntrX genes, respectively. The amino acid sequences of A. brasilense NtrY and NtrX proteins showed high similarity to sensor/kinase and regulatory proteins, respectively. Analysis of lacZ transcriptional fusions by the ß-galactosidase assay in Escherichia coli ntrC mutants showed that the NtrY/NtrX proteins failed to activate transcription of the nifA promoter of A. brasilense. The ntrYX operon complemented a nifR3ntrBC deletion mutant of A. brasilense for nitrate-dependent growth, suggesting a possible cross-talk between the NtrY/X and NtrB/C sensor/regulator pairs. Our data support the existence of another two-component regulatory system in A. brasilense, the NtrY/NtrX system, probably involved in the regulation of nitrate assimilation.
publishDate 2002
dc.date.none.fl_str_mv 2002-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X2002000600004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.35 n.6 2002
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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