Conventional cytogenetic characterization of a new cell line, ACP01, established from a primary human gastric tumor
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200008 |
Resumo: | Gastric cancer is the second most frequent type of neoplasia and also the second most important cause of death in the world. Virtually all the established cell lines of gastric neoplasia were developed in Asian countries, and western countries have contributed very little to this area. In the present study we describe the establishment of the cell line ACP01 and characterize it cytogenetically by means of in vitro immortalization. Cells were transformed from an intestinal-type gastric adenocarcinoma (T4N2M0) originating from a 48-year-old male patient. This is the first gastric adenocarcinoma cell line established in Brazil. The most powerful application of the cell line ACP01 is in the assessment of cytotoxicity. Solid tumor cell lines from different origins have been treated with several conventional and investigational anticancer drugs. The ACP01 cell line is triploid, grows as a single, non-organized layer, similar to fibroblasts, with focus formation, heterogeneous division, and a cell cycle of approximately 40 h. Chromosome 8 trisomy, present in 60% of the cells, was the most frequent cytogenetic alteration. These data lead us to propose a multifactorial triggering of gastric cancer which evolves over multiple stages involving progressive genetic changes and clonal expansion. |
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Conventional cytogenetic characterization of a new cell line, ACP01, established from a primary human gastric tumorCell line ACP01Human gastric adenocarcinomaIn vitro immortalizationChromosome 8 trisomyClonal expansionGastric cancer is the second most frequent type of neoplasia and also the second most important cause of death in the world. Virtually all the established cell lines of gastric neoplasia were developed in Asian countries, and western countries have contributed very little to this area. In the present study we describe the establishment of the cell line ACP01 and characterize it cytogenetically by means of in vitro immortalization. Cells were transformed from an intestinal-type gastric adenocarcinoma (T4N2M0) originating from a 48-year-old male patient. This is the first gastric adenocarcinoma cell line established in Brazil. The most powerful application of the cell line ACP01 is in the assessment of cytotoxicity. Solid tumor cell lines from different origins have been treated with several conventional and investigational anticancer drugs. The ACP01 cell line is triploid, grows as a single, non-organized layer, similar to fibroblasts, with focus formation, heterogeneous division, and a cell cycle of approximately 40 h. Chromosome 8 trisomy, present in 60% of the cells, was the most frequent cytogenetic alteration. These data lead us to propose a multifactorial triggering of gastric cancer which evolves over multiple stages involving progressive genetic changes and clonal expansion.Associação Brasileira de Divulgação Científica2004-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200008Brazilian Journal of Medical and Biological Research v.37 n.12 2004reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2004001200008info:eu-repo/semantics/openAccessLima,E.M.Rissino,J.D.Harada,M.L.Assumpção,P.P.Demachki,S.Guimarães,A.C.Casartelli,C.Smith,M.A.C.Burbano,R.R.eng2004-11-17T00:00:00Zoai:scielo:S0100-879X2004001200008Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2004-11-17T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Conventional cytogenetic characterization of a new cell line, ACP01, established from a primary human gastric tumor |
title |
Conventional cytogenetic characterization of a new cell line, ACP01, established from a primary human gastric tumor |
spellingShingle |
Conventional cytogenetic characterization of a new cell line, ACP01, established from a primary human gastric tumor Lima,E.M. Cell line ACP01 Human gastric adenocarcinoma In vitro immortalization Chromosome 8 trisomy Clonal expansion |
title_short |
Conventional cytogenetic characterization of a new cell line, ACP01, established from a primary human gastric tumor |
title_full |
Conventional cytogenetic characterization of a new cell line, ACP01, established from a primary human gastric tumor |
title_fullStr |
Conventional cytogenetic characterization of a new cell line, ACP01, established from a primary human gastric tumor |
title_full_unstemmed |
Conventional cytogenetic characterization of a new cell line, ACP01, established from a primary human gastric tumor |
title_sort |
Conventional cytogenetic characterization of a new cell line, ACP01, established from a primary human gastric tumor |
author |
Lima,E.M. |
author_facet |
Lima,E.M. Rissino,J.D. Harada,M.L. Assumpção,P.P. Demachki,S. Guimarães,A.C. Casartelli,C. Smith,M.A.C. Burbano,R.R. |
author_role |
author |
author2 |
Rissino,J.D. Harada,M.L. Assumpção,P.P. Demachki,S. Guimarães,A.C. Casartelli,C. Smith,M.A.C. Burbano,R.R. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lima,E.M. Rissino,J.D. Harada,M.L. Assumpção,P.P. Demachki,S. Guimarães,A.C. Casartelli,C. Smith,M.A.C. Burbano,R.R. |
dc.subject.por.fl_str_mv |
Cell line ACP01 Human gastric adenocarcinoma In vitro immortalization Chromosome 8 trisomy Clonal expansion |
topic |
Cell line ACP01 Human gastric adenocarcinoma In vitro immortalization Chromosome 8 trisomy Clonal expansion |
description |
Gastric cancer is the second most frequent type of neoplasia and also the second most important cause of death in the world. Virtually all the established cell lines of gastric neoplasia were developed in Asian countries, and western countries have contributed very little to this area. In the present study we describe the establishment of the cell line ACP01 and characterize it cytogenetically by means of in vitro immortalization. Cells were transformed from an intestinal-type gastric adenocarcinoma (T4N2M0) originating from a 48-year-old male patient. This is the first gastric adenocarcinoma cell line established in Brazil. The most powerful application of the cell line ACP01 is in the assessment of cytotoxicity. Solid tumor cell lines from different origins have been treated with several conventional and investigational anticancer drugs. The ACP01 cell line is triploid, grows as a single, non-organized layer, similar to fibroblasts, with focus formation, heterogeneous division, and a cell cycle of approximately 40 h. Chromosome 8 trisomy, present in 60% of the cells, was the most frequent cytogenetic alteration. These data lead us to propose a multifactorial triggering of gastric cancer which evolves over multiple stages involving progressive genetic changes and clonal expansion. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-879X2004001200008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.37 n.12 2004 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302933436989440 |