Myoglobin entrapment in poly(vinyl alcohol) dense membranes
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322014000300017 |
Resumo: | Our goal in this study was the immobilization of myoglobin in poly(vinyl alcohol) dense membranes. Glutaraldehyde was investigated both as the crosslinking agent, aiming to increase the membrane stability in aqueous medium, and as the vehicle to bind myoglobin and PVA. Reaction and membrane synthesis were carried simultaneously in mild operating conditions in order to maintain the native protein folding. Membrane characterization comprised the water swelling degree, DSC, TGA, UV-visible spectroscopy, FTIR analysis and oxygen transport in a dialysis cell. The incorporation of myoglobin in the film decreased the water swelling degree and improved the membrane thermal properties compared to unmodified PVA membrane. The reduction of ferric iron in the prosthetic group of the protein to the ferrous form was observed. The increased affinity between oxygen and the immobilized myoglobin did not favor the release of this solute from the biocarrier. |
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Brazilian Journal of Chemical Engineering |
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Myoglobin entrapment in poly(vinyl alcohol) dense membranesMyoglobinOxygen/nitrogen separationPoly(vinyl alcohol)MembranesImmobilizationOur goal in this study was the immobilization of myoglobin in poly(vinyl alcohol) dense membranes. Glutaraldehyde was investigated both as the crosslinking agent, aiming to increase the membrane stability in aqueous medium, and as the vehicle to bind myoglobin and PVA. Reaction and membrane synthesis were carried simultaneously in mild operating conditions in order to maintain the native protein folding. Membrane characterization comprised the water swelling degree, DSC, TGA, UV-visible spectroscopy, FTIR analysis and oxygen transport in a dialysis cell. The incorporation of myoglobin in the film decreased the water swelling degree and improved the membrane thermal properties compared to unmodified PVA membrane. The reduction of ferric iron in the prosthetic group of the protein to the ferrous form was observed. The increased affinity between oxygen and the immobilized myoglobin did not favor the release of this solute from the biocarrier.Brazilian Society of Chemical Engineering2014-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322014000300017Brazilian Journal of Chemical Engineering v.31 n.3 2014reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/0104-6632.20140313s00002307info:eu-repo/semantics/openAccessFigueiredo,K. C. S.Alves,T. L. M.Borges,C. P.eng2014-09-17T00:00:00Zoai:scielo:S0104-66322014000300017Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2014-09-17T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
Myoglobin entrapment in poly(vinyl alcohol) dense membranes |
title |
Myoglobin entrapment in poly(vinyl alcohol) dense membranes |
spellingShingle |
Myoglobin entrapment in poly(vinyl alcohol) dense membranes Figueiredo,K. C. S. Myoglobin Oxygen/nitrogen separation Poly(vinyl alcohol) Membranes Immobilization |
title_short |
Myoglobin entrapment in poly(vinyl alcohol) dense membranes |
title_full |
Myoglobin entrapment in poly(vinyl alcohol) dense membranes |
title_fullStr |
Myoglobin entrapment in poly(vinyl alcohol) dense membranes |
title_full_unstemmed |
Myoglobin entrapment in poly(vinyl alcohol) dense membranes |
title_sort |
Myoglobin entrapment in poly(vinyl alcohol) dense membranes |
author |
Figueiredo,K. C. S. |
author_facet |
Figueiredo,K. C. S. Alves,T. L. M. Borges,C. P. |
author_role |
author |
author2 |
Alves,T. L. M. Borges,C. P. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Figueiredo,K. C. S. Alves,T. L. M. Borges,C. P. |
dc.subject.por.fl_str_mv |
Myoglobin Oxygen/nitrogen separation Poly(vinyl alcohol) Membranes Immobilization |
topic |
Myoglobin Oxygen/nitrogen separation Poly(vinyl alcohol) Membranes Immobilization |
description |
Our goal in this study was the immobilization of myoglobin in poly(vinyl alcohol) dense membranes. Glutaraldehyde was investigated both as the crosslinking agent, aiming to increase the membrane stability in aqueous medium, and as the vehicle to bind myoglobin and PVA. Reaction and membrane synthesis were carried simultaneously in mild operating conditions in order to maintain the native protein folding. Membrane characterization comprised the water swelling degree, DSC, TGA, UV-visible spectroscopy, FTIR analysis and oxygen transport in a dialysis cell. The incorporation of myoglobin in the film decreased the water swelling degree and improved the membrane thermal properties compared to unmodified PVA membrane. The reduction of ferric iron in the prosthetic group of the protein to the ferrous form was observed. The increased affinity between oxygen and the immobilized myoglobin did not favor the release of this solute from the biocarrier. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322014000300017 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322014000300017 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0104-6632.20140313s00002307 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.31 n.3 2014 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213174343630848 |