Media optimization for β-Fructofuranosidase production by Aspergillus oryzae
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322012000100006 |
Resumo: | β-Fructofuranosidase production by Aspergillus oryzae IPT301 was maximized in shake flasks. Response Surface Methodology (RSM) involving Small Central Composite Design was adopted to evaluate the fructosyltransferase (FTase) activity by changing three medium component concentrations: sucrose, urea and yeast extract. The optimal set of conditions for maximum fructosyltransferase production was as follows: sucrose 320.5 g/L, urea 7.13 g/L and yeast extract 2.11 g/L. In this optimal condition, the following improvements were achieved: an increase of 48.8% in cell growth, 112% and 62% in micelial and free FTase activities, respectively, 62.8% in the ratio of fructosyltransferase/hydrolytic activities for enzyme linked to mycelium and 67.5% for free enzyme. |
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Brazilian Journal of Chemical Engineering |
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Media optimization for β-Fructofuranosidase production by Aspergillus oryzaeFructosyltransferaseFructofuranosidaseFructooligosaccharidesAspergillus oryzaeExperimental Designβ-Fructofuranosidase production by Aspergillus oryzae IPT301 was maximized in shake flasks. Response Surface Methodology (RSM) involving Small Central Composite Design was adopted to evaluate the fructosyltransferase (FTase) activity by changing three medium component concentrations: sucrose, urea and yeast extract. The optimal set of conditions for maximum fructosyltransferase production was as follows: sucrose 320.5 g/L, urea 7.13 g/L and yeast extract 2.11 g/L. In this optimal condition, the following improvements were achieved: an increase of 48.8% in cell growth, 112% and 62% in micelial and free FTase activities, respectively, 62.8% in the ratio of fructosyltransferase/hydrolytic activities for enzyme linked to mycelium and 67.5% for free enzyme.Brazilian Society of Chemical Engineering2012-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322012000100006Brazilian Journal of Chemical Engineering v.29 n.1 2012reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322012000100006info:eu-repo/semantics/openAccessOttoni,C. A.Cuervo-Fernández,R.Piccoli,R. M.Moreira,R.Guilarte-Maresma,B.Silva,E. Sabino daRodrigues,M. F. A.Maiorano,A. E.eng2012-03-08T00:00:00Zoai:scielo:S0104-66322012000100006Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2012-03-08T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
Media optimization for β-Fructofuranosidase production by Aspergillus oryzae |
title |
Media optimization for β-Fructofuranosidase production by Aspergillus oryzae |
spellingShingle |
Media optimization for β-Fructofuranosidase production by Aspergillus oryzae Ottoni,C. A. Fructosyltransferase Fructofuranosidase Fructooligosaccharides Aspergillus oryzae Experimental Design |
title_short |
Media optimization for β-Fructofuranosidase production by Aspergillus oryzae |
title_full |
Media optimization for β-Fructofuranosidase production by Aspergillus oryzae |
title_fullStr |
Media optimization for β-Fructofuranosidase production by Aspergillus oryzae |
title_full_unstemmed |
Media optimization for β-Fructofuranosidase production by Aspergillus oryzae |
title_sort |
Media optimization for β-Fructofuranosidase production by Aspergillus oryzae |
author |
Ottoni,C. A. |
author_facet |
Ottoni,C. A. Cuervo-Fernández,R. Piccoli,R. M. Moreira,R. Guilarte-Maresma,B. Silva,E. Sabino da Rodrigues,M. F. A. Maiorano,A. E. |
author_role |
author |
author2 |
Cuervo-Fernández,R. Piccoli,R. M. Moreira,R. Guilarte-Maresma,B. Silva,E. Sabino da Rodrigues,M. F. A. Maiorano,A. E. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Ottoni,C. A. Cuervo-Fernández,R. Piccoli,R. M. Moreira,R. Guilarte-Maresma,B. Silva,E. Sabino da Rodrigues,M. F. A. Maiorano,A. E. |
dc.subject.por.fl_str_mv |
Fructosyltransferase Fructofuranosidase Fructooligosaccharides Aspergillus oryzae Experimental Design |
topic |
Fructosyltransferase Fructofuranosidase Fructooligosaccharides Aspergillus oryzae Experimental Design |
description |
β-Fructofuranosidase production by Aspergillus oryzae IPT301 was maximized in shake flasks. Response Surface Methodology (RSM) involving Small Central Composite Design was adopted to evaluate the fructosyltransferase (FTase) activity by changing three medium component concentrations: sucrose, urea and yeast extract. The optimal set of conditions for maximum fructosyltransferase production was as follows: sucrose 320.5 g/L, urea 7.13 g/L and yeast extract 2.11 g/L. In this optimal condition, the following improvements were achieved: an increase of 48.8% in cell growth, 112% and 62% in micelial and free FTase activities, respectively, 62.8% in the ratio of fructosyltransferase/hydrolytic activities for enzyme linked to mycelium and 67.5% for free enzyme. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322012000100006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322012000100006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0104-66322012000100006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.29 n.1 2012 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213173522595840 |