HIGHLY EFFICIENT PRODUCTION OF L (+)-LACTIC ACID USING MEDIUM WITH POTATO, CORN STEEP LIQUOR AND CALCIUM CARBONATE BY Lactobacillus rhamnosus ATCC 9595

• Autor(a) principal: Cunha,Marília Crivelari da
• Data de Publicação: 2018
• Outros Autores: Masotti,Michelle Thiemi, Mondragón-Bernal,Olga Lucía, Alves,José Guilherme Lembi Ferreira
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Chemical Engineering
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322018000300887
• topic: Lactic acid; Fermentation; Potato flour; Corn steep liquor; Response surface

Abstract The objective of this study was to optimize the composition of the fermentation medium through a Central Composite Rotational Design (CCRD) for the production of L (+)- latic acid (LA) using Lactobacillus rhamnosus ATCC 9595 and potato, corn steep liquor (CSL) and calcium carbonate as raw materials. A CCRD with three independent variables (potato flour (PF), CSL and CaCO3 concentrations) was performed for a total of 17 treatments. Kinetic studies were conducted, and samples were taken at time intervals of 0 to 72 h. Cell count, pH, reducing sugars and LA concentrations were determined. LA productivity and yield, sugar consumption and cell growth factor were calculated. The highest concentration of LA was 183.8 g/L, with 2.553 g/L.h productivity, 94.2% yield and 96.1 % sugar consumption.