CAROTENOID PRODUCTION BY Sporidiobolus pararoseus IN AGROINDUSTRIAL MEDIUM: OPTIMIZATION OF CULTURE CONDITIONS IN SHAKE FLASKS AND SCALE-UP IN A STIRRED TANK FERMENTER
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322018000200509 |
Resumo: | Abstract Biotechnological production of carotenoids can be affected by cultivation conditions, such as temperature, pH and agitation. The aim of this study was to maximize carotenoid production by Sporidiobolus pararoseus in shake flasks with agroindustrial by-products as substrates. The best conditions were used in a stirred tank fermenter. The medium consisted of corn steep liquor and sugar cane molasses pretreated with sulfuric acid. In order to evaluate the effects of the variables, a central composite rotatable design was used. The highest values of total carotenoids (565 µg L-1), biomass (13.6 g L-1) and Yp/s (10.9 µg g-1) were obtained in Assay 13 at 27.5ºC, 150 rpm and pH=4, in Erlenmeyer flasks. For the best conditions defined for carotenoid productivity and 1.2 vvm in the stirred tank fermenter, the maximum value was 1969.3 µg L-1 of total carotenoids. It was 3.5-fold higher than the value obtained when shake flasks were used. |
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Brazilian Journal of Chemical Engineering |
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CAROTENOID PRODUCTION BY Sporidiobolus pararoseus IN AGROINDUSTRIAL MEDIUM: OPTIMIZATION OF CULTURE CONDITIONS IN SHAKE FLASKS AND SCALE-UP IN A STIRRED TANK FERMENTERyeastmolassesexperimental designAbstract Biotechnological production of carotenoids can be affected by cultivation conditions, such as temperature, pH and agitation. The aim of this study was to maximize carotenoid production by Sporidiobolus pararoseus in shake flasks with agroindustrial by-products as substrates. The best conditions were used in a stirred tank fermenter. The medium consisted of corn steep liquor and sugar cane molasses pretreated with sulfuric acid. In order to evaluate the effects of the variables, a central composite rotatable design was used. The highest values of total carotenoids (565 µg L-1), biomass (13.6 g L-1) and Yp/s (10.9 µg g-1) were obtained in Assay 13 at 27.5ºC, 150 rpm and pH=4, in Erlenmeyer flasks. For the best conditions defined for carotenoid productivity and 1.2 vvm in the stirred tank fermenter, the maximum value was 1969.3 µg L-1 of total carotenoids. It was 3.5-fold higher than the value obtained when shake flasks were used.Brazilian Society of Chemical Engineering2018-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322018000200509Brazilian Journal of Chemical Engineering v.35 n.2 2018reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/0104-6632.20180352s20160545info:eu-repo/semantics/openAccessBorba,Carina MolinsTavares,Millene das NevesMoraes,Caroline CostaBurkert,Janaína Fernandes de Medeiroseng2018-09-17T00:00:00Zoai:scielo:S0104-66322018000200509Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2018-09-17T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
CAROTENOID PRODUCTION BY Sporidiobolus pararoseus IN AGROINDUSTRIAL MEDIUM: OPTIMIZATION OF CULTURE CONDITIONS IN SHAKE FLASKS AND SCALE-UP IN A STIRRED TANK FERMENTER |
title |
CAROTENOID PRODUCTION BY Sporidiobolus pararoseus IN AGROINDUSTRIAL MEDIUM: OPTIMIZATION OF CULTURE CONDITIONS IN SHAKE FLASKS AND SCALE-UP IN A STIRRED TANK FERMENTER |
spellingShingle |
CAROTENOID PRODUCTION BY Sporidiobolus pararoseus IN AGROINDUSTRIAL MEDIUM: OPTIMIZATION OF CULTURE CONDITIONS IN SHAKE FLASKS AND SCALE-UP IN A STIRRED TANK FERMENTER Borba,Carina Molins yeast molasses experimental design |
title_short |
CAROTENOID PRODUCTION BY Sporidiobolus pararoseus IN AGROINDUSTRIAL MEDIUM: OPTIMIZATION OF CULTURE CONDITIONS IN SHAKE FLASKS AND SCALE-UP IN A STIRRED TANK FERMENTER |
title_full |
CAROTENOID PRODUCTION BY Sporidiobolus pararoseus IN AGROINDUSTRIAL MEDIUM: OPTIMIZATION OF CULTURE CONDITIONS IN SHAKE FLASKS AND SCALE-UP IN A STIRRED TANK FERMENTER |
title_fullStr |
CAROTENOID PRODUCTION BY Sporidiobolus pararoseus IN AGROINDUSTRIAL MEDIUM: OPTIMIZATION OF CULTURE CONDITIONS IN SHAKE FLASKS AND SCALE-UP IN A STIRRED TANK FERMENTER |
title_full_unstemmed |
CAROTENOID PRODUCTION BY Sporidiobolus pararoseus IN AGROINDUSTRIAL MEDIUM: OPTIMIZATION OF CULTURE CONDITIONS IN SHAKE FLASKS AND SCALE-UP IN A STIRRED TANK FERMENTER |
title_sort |
CAROTENOID PRODUCTION BY Sporidiobolus pararoseus IN AGROINDUSTRIAL MEDIUM: OPTIMIZATION OF CULTURE CONDITIONS IN SHAKE FLASKS AND SCALE-UP IN A STIRRED TANK FERMENTER |
author |
Borba,Carina Molins |
author_facet |
Borba,Carina Molins Tavares,Millene das Neves Moraes,Caroline Costa Burkert,Janaína Fernandes de Medeiros |
author_role |
author |
author2 |
Tavares,Millene das Neves Moraes,Caroline Costa Burkert,Janaína Fernandes de Medeiros |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Borba,Carina Molins Tavares,Millene das Neves Moraes,Caroline Costa Burkert,Janaína Fernandes de Medeiros |
dc.subject.por.fl_str_mv |
yeast molasses experimental design |
topic |
yeast molasses experimental design |
description |
Abstract Biotechnological production of carotenoids can be affected by cultivation conditions, such as temperature, pH and agitation. The aim of this study was to maximize carotenoid production by Sporidiobolus pararoseus in shake flasks with agroindustrial by-products as substrates. The best conditions were used in a stirred tank fermenter. The medium consisted of corn steep liquor and sugar cane molasses pretreated with sulfuric acid. In order to evaluate the effects of the variables, a central composite rotatable design was used. The highest values of total carotenoids (565 µg L-1), biomass (13.6 g L-1) and Yp/s (10.9 µg g-1) were obtained in Assay 13 at 27.5ºC, 150 rpm and pH=4, in Erlenmeyer flasks. For the best conditions defined for carotenoid productivity and 1.2 vvm in the stirred tank fermenter, the maximum value was 1969.3 µg L-1 of total carotenoids. It was 3.5-fold higher than the value obtained when shake flasks were used. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322018000200509 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322018000200509 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0104-6632.20180352s20160545 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.35 n.2 2018 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213175912300544 |