Soluble microbial product (SMP) characterization in bench-scale aerobic and anaerobic CSTRs under different operational conditions
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322010000100009 |
Resumo: | This work presents results on the production and characterization (by both mass spectrometry and conventional chemical analyses) of Soluble Microbial Products (SMP) that accumulated in aerobic and anaerobic bench scale completely stirred tank reactors (CSTRs) fed with glucose or acetate under different hydraulic retention times (HRT) and temperatures. SMP accumulation varied from 2 to 68% of the influent COD in the aerobic reactor and from 9 to 27% in the anaerobic reactor and increased with the decrease in temperature and with the HRT reduction in the aerobic reactor. On the other hand, in the anaerobic reactor, the organic loading rate and the temperature had little impact on SMP production, implying that the SMP originated from different mechanisms in each system. For both reactors, a higher accumulation of SMPs was observed as the substrate was acetate when compared to glucose, and the chemical analysis showed that the majority of the SMP did not seem to be proteins or carbohydrates. Principal component analysis (PCA) of the mass spectra from positive and negative mode electron-spray ionization (LC-IT-TOF-MS) and results from matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) confirmed the chemical analyses and showed the absence of proteins in the effluents and the predominance of low molecular weight SMP. The PCA analysis also showed that the majority of the SMP from aerobic and anaerobic reactors did not seem to originate from soluble extracellular polymeric substances (EPS) or cell lysis products. |
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Soluble microbial product (SMP) characterization in bench-scale aerobic and anaerobic CSTRs under different operational conditionsBiological wastewater treatmentSoluble microbial productsResidual CODVolatile fatty acidsmass spectrometryThis work presents results on the production and characterization (by both mass spectrometry and conventional chemical analyses) of Soluble Microbial Products (SMP) that accumulated in aerobic and anaerobic bench scale completely stirred tank reactors (CSTRs) fed with glucose or acetate under different hydraulic retention times (HRT) and temperatures. SMP accumulation varied from 2 to 68% of the influent COD in the aerobic reactor and from 9 to 27% in the anaerobic reactor and increased with the decrease in temperature and with the HRT reduction in the aerobic reactor. On the other hand, in the anaerobic reactor, the organic loading rate and the temperature had little impact on SMP production, implying that the SMP originated from different mechanisms in each system. For both reactors, a higher accumulation of SMPs was observed as the substrate was acetate when compared to glucose, and the chemical analysis showed that the majority of the SMP did not seem to be proteins or carbohydrates. Principal component analysis (PCA) of the mass spectra from positive and negative mode electron-spray ionization (LC-IT-TOF-MS) and results from matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) confirmed the chemical analyses and showed the absence of proteins in the effluents and the predominance of low molecular weight SMP. The PCA analysis also showed that the majority of the SMP from aerobic and anaerobic reactors did not seem to originate from soluble extracellular polymeric substances (EPS) or cell lysis products.Brazilian Society of Chemical Engineering2010-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322010000100009Brazilian Journal of Chemical Engineering v.27 n.1 2010reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322010000100009info:eu-repo/semantics/openAccessMesquita,P. L.Aquino,S. F.Xavier,A. L. P.Silva,J. C. Cardoso daAfonso,R. C. F.Silva,S. Queirozeng2010-04-14T00:00:00Zoai:scielo:S0104-66322010000100009Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2010-04-14T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
Soluble microbial product (SMP) characterization in bench-scale aerobic and anaerobic CSTRs under different operational conditions |
title |
Soluble microbial product (SMP) characterization in bench-scale aerobic and anaerobic CSTRs under different operational conditions |
spellingShingle |
Soluble microbial product (SMP) characterization in bench-scale aerobic and anaerobic CSTRs under different operational conditions Mesquita,P. L. Biological wastewater treatment Soluble microbial products Residual COD Volatile fatty acids mass spectrometry |
title_short |
Soluble microbial product (SMP) characterization in bench-scale aerobic and anaerobic CSTRs under different operational conditions |
title_full |
Soluble microbial product (SMP) characterization in bench-scale aerobic and anaerobic CSTRs under different operational conditions |
title_fullStr |
Soluble microbial product (SMP) characterization in bench-scale aerobic and anaerobic CSTRs under different operational conditions |
title_full_unstemmed |
Soluble microbial product (SMP) characterization in bench-scale aerobic and anaerobic CSTRs under different operational conditions |
title_sort |
Soluble microbial product (SMP) characterization in bench-scale aerobic and anaerobic CSTRs under different operational conditions |
author |
Mesquita,P. L. |
author_facet |
Mesquita,P. L. Aquino,S. F. Xavier,A. L. P. Silva,J. C. Cardoso da Afonso,R. C. F. Silva,S. Queiroz |
author_role |
author |
author2 |
Aquino,S. F. Xavier,A. L. P. Silva,J. C. Cardoso da Afonso,R. C. F. Silva,S. Queiroz |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Mesquita,P. L. Aquino,S. F. Xavier,A. L. P. Silva,J. C. Cardoso da Afonso,R. C. F. Silva,S. Queiroz |
dc.subject.por.fl_str_mv |
Biological wastewater treatment Soluble microbial products Residual COD Volatile fatty acids mass spectrometry |
topic |
Biological wastewater treatment Soluble microbial products Residual COD Volatile fatty acids mass spectrometry |
description |
This work presents results on the production and characterization (by both mass spectrometry and conventional chemical analyses) of Soluble Microbial Products (SMP) that accumulated in aerobic and anaerobic bench scale completely stirred tank reactors (CSTRs) fed with glucose or acetate under different hydraulic retention times (HRT) and temperatures. SMP accumulation varied from 2 to 68% of the influent COD in the aerobic reactor and from 9 to 27% in the anaerobic reactor and increased with the decrease in temperature and with the HRT reduction in the aerobic reactor. On the other hand, in the anaerobic reactor, the organic loading rate and the temperature had little impact on SMP production, implying that the SMP originated from different mechanisms in each system. For both reactors, a higher accumulation of SMPs was observed as the substrate was acetate when compared to glucose, and the chemical analysis showed that the majority of the SMP did not seem to be proteins or carbohydrates. Principal component analysis (PCA) of the mass spectra from positive and negative mode electron-spray ionization (LC-IT-TOF-MS) and results from matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) confirmed the chemical analyses and showed the absence of proteins in the effluents and the predominance of low molecular weight SMP. The PCA analysis also showed that the majority of the SMP from aerobic and anaerobic reactors did not seem to originate from soluble extracellular polymeric substances (EPS) or cell lysis products. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322010000100009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322010000100009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0104-66322010000100009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.27 n.1 2010 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213173090582528 |