PRODUCTION OF CELLULOLYTIC ENZYMES BY Gelatoporia subvermispora USING DIFFERENT SUBSTRATES

Detalhes bibliográficos
Autor(a) principal: Loureiro,Cristiane Bianchi
Data de Publicação: 2018
Outros Autores: Gasparotto,Juliana Machado, Rabuscke,Caroline M., Baldoni,Daiana Bortoluzzi, Guedes,Jerson V. Carús, Mazutti,Marcio A., Jacques,Rodrigo J. Seminoti
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Chemical Engineering
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322018000200459
Resumo: Abstract In this study the solid-state fermentation for production of cellulolytic enzymes by the fungus Gelatoporia subvermispora was optimized. The enzyme activities on filter paper, exo-cellulase, endo-cellulase and xylanase were determined. A Plackett-Burman design (PB) was used to determine the most significant variables (moisture content of substrate, inoculum density, corn steep liquor concentration, pH and peptone concentration) in the enzyme production for each substrate (sugarcane bagasse, sewage sludge and rice straw). The highest value for filter paper activity was obtained using sugarcane bagasse as substrate. The sewage sludge was an excellent medium for the production of xylanase and exo-cellulases. The endo-cellulase activity was similar in all substrates tested. This is the first report of the Gelatoporia genus for the production of cellulolytic enzymes, being a promising strain for this purpose.
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spelling PRODUCTION OF CELLULOLYTIC ENZYMES BY Gelatoporia subvermispora USING DIFFERENT SUBSTRATESPampa biomeCellulasesLignocellulosic wastesSolid state fermentationAbstract In this study the solid-state fermentation for production of cellulolytic enzymes by the fungus Gelatoporia subvermispora was optimized. The enzyme activities on filter paper, exo-cellulase, endo-cellulase and xylanase were determined. A Plackett-Burman design (PB) was used to determine the most significant variables (moisture content of substrate, inoculum density, corn steep liquor concentration, pH and peptone concentration) in the enzyme production for each substrate (sugarcane bagasse, sewage sludge and rice straw). The highest value for filter paper activity was obtained using sugarcane bagasse as substrate. The sewage sludge was an excellent medium for the production of xylanase and exo-cellulases. The endo-cellulase activity was similar in all substrates tested. This is the first report of the Gelatoporia genus for the production of cellulolytic enzymes, being a promising strain for this purpose.Brazilian Society of Chemical Engineering2018-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322018000200459Brazilian Journal of Chemical Engineering v.35 n.2 2018reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/0104-6632.20180352s20160575info:eu-repo/semantics/openAccessLoureiro,Cristiane BianchiGasparotto,Juliana MachadoRabuscke,Caroline M.Baldoni,Daiana BortoluzziGuedes,Jerson V. CarúsMazutti,Marcio A.Jacques,Rodrigo J. Seminotieng2018-09-17T00:00:00Zoai:scielo:S0104-66322018000200459Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2018-09-17T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false
dc.title.none.fl_str_mv PRODUCTION OF CELLULOLYTIC ENZYMES BY Gelatoporia subvermispora USING DIFFERENT SUBSTRATES
title PRODUCTION OF CELLULOLYTIC ENZYMES BY Gelatoporia subvermispora USING DIFFERENT SUBSTRATES
spellingShingle PRODUCTION OF CELLULOLYTIC ENZYMES BY Gelatoporia subvermispora USING DIFFERENT SUBSTRATES
Loureiro,Cristiane Bianchi
Pampa biome
Cellulases
Lignocellulosic wastes
Solid state fermentation
title_short PRODUCTION OF CELLULOLYTIC ENZYMES BY Gelatoporia subvermispora USING DIFFERENT SUBSTRATES
title_full PRODUCTION OF CELLULOLYTIC ENZYMES BY Gelatoporia subvermispora USING DIFFERENT SUBSTRATES
title_fullStr PRODUCTION OF CELLULOLYTIC ENZYMES BY Gelatoporia subvermispora USING DIFFERENT SUBSTRATES
title_full_unstemmed PRODUCTION OF CELLULOLYTIC ENZYMES BY Gelatoporia subvermispora USING DIFFERENT SUBSTRATES
title_sort PRODUCTION OF CELLULOLYTIC ENZYMES BY Gelatoporia subvermispora USING DIFFERENT SUBSTRATES
author Loureiro,Cristiane Bianchi
author_facet Loureiro,Cristiane Bianchi
Gasparotto,Juliana Machado
Rabuscke,Caroline M.
Baldoni,Daiana Bortoluzzi
Guedes,Jerson V. Carús
Mazutti,Marcio A.
Jacques,Rodrigo J. Seminoti
author_role author
author2 Gasparotto,Juliana Machado
Rabuscke,Caroline M.
Baldoni,Daiana Bortoluzzi
Guedes,Jerson V. Carús
Mazutti,Marcio A.
Jacques,Rodrigo J. Seminoti
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Loureiro,Cristiane Bianchi
Gasparotto,Juliana Machado
Rabuscke,Caroline M.
Baldoni,Daiana Bortoluzzi
Guedes,Jerson V. Carús
Mazutti,Marcio A.
Jacques,Rodrigo J. Seminoti
dc.subject.por.fl_str_mv Pampa biome
Cellulases
Lignocellulosic wastes
Solid state fermentation
topic Pampa biome
Cellulases
Lignocellulosic wastes
Solid state fermentation
description Abstract In this study the solid-state fermentation for production of cellulolytic enzymes by the fungus Gelatoporia subvermispora was optimized. The enzyme activities on filter paper, exo-cellulase, endo-cellulase and xylanase were determined. A Plackett-Burman design (PB) was used to determine the most significant variables (moisture content of substrate, inoculum density, corn steep liquor concentration, pH and peptone concentration) in the enzyme production for each substrate (sugarcane bagasse, sewage sludge and rice straw). The highest value for filter paper activity was obtained using sugarcane bagasse as substrate. The sewage sludge was an excellent medium for the production of xylanase and exo-cellulases. The endo-cellulase activity was similar in all substrates tested. This is the first report of the Gelatoporia genus for the production of cellulolytic enzymes, being a promising strain for this purpose.
publishDate 2018
dc.date.none.fl_str_mv 2018-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322018000200459
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322018000200459
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0104-6632.20180352s20160575
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
dc.source.none.fl_str_mv Brazilian Journal of Chemical Engineering v.35 n.2 2018
reponame:Brazilian Journal of Chemical Engineering
instname:Associação Brasileira de Engenharia Química (ABEQ)
instacron:ABEQ
instname_str Associação Brasileira de Engenharia Química (ABEQ)
instacron_str ABEQ
institution ABEQ
reponame_str Brazilian Journal of Chemical Engineering
collection Brazilian Journal of Chemical Engineering
repository.name.fl_str_mv Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)
repository.mail.fl_str_mv rgiudici@usp.br||rgiudici@usp.br
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