Bacterial Cellulose Production by Komagataeibacter hansenii ATCC 23769 Using Sisal Juice - An Agroindustry Waste

• Autor(a) principal: Lima,H. L. S.
• Data de Publicação: 2017
• Outros Autores: Nascimento,E. S., Andrade,F. K., Brígida,A. I. S., Borges,M. F., Cassales,A. R., Muniz,C. R., Souza Filho,M. de S. M., Morais,J. P. S., Rosa,M. de F.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Chemical Engineering
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322017000300671
• topic: Bacterial cellulose; Sisal juice; Komagataeibacter hansenii; Agroindustrial waste; Nanotechnology

ABSTRACT In this study, we have optimized production of bacterial cellulose (BC) by Komagataeibacter hansenii ATCC 23769 in a static cultivation using sisal juice, an agroindustrial residue, as substrate. Optimization of fermentation parameters has been carried out using the one-variable-at-a-time method. Effect of initial sugar concentration, pH, nitrogen supplement, and cultivation time was evaluated. The influence of nitrogen source and quantity for bacterial cellulose production was studied using a central composite rotational design (CCRD).The highest production of BC (3.38 g/L) was obtained after 10 days of cultivation, using sisal juice (pH 5) at 15 g/L of sugars and supplemented with 7.5 g/L of extract yeast. The cellulose production yield in selected sisal culture conditions was three times higher than the yield in synthetic medium, indicating that sisal juice is a suitable substrate for BC production.