Real time and in situ Near-Infrared Spectroscopy (Nirs) for Quantitative Monitoring of Biomass, Glucose, Ethanol and Glycerine concentrations in an alcoholic fermentation
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322017000200459 |
Resumo: | Abstract This study investigates the application of NIR spectroscopy for real-time monitoring of process parameters in alcoholic fermentations. For this purpose, fermentation batches using Saccharomyces cerevisiae were carried out and monitored in situ by an immersed NIRS probe. Samples were collected throughout the fermentations, and concentrations of biomass, glucose, ethanol and glycerin were analyzed by reference methods and multivariate data analyses such as PCA and PLSR. PCA was used to investigate data variability and to select samples for calibration and for external validation sets. A PLSR model for biomass yielded R2 and RMSEP values of 0.99 and 0.276 g.L-1, respectively. For glucose, the carbon source, the PLSR model yielded an R2 of 0.95 and an RMSEP of 7.14 g.L-1. The PLSR model for ethanol was characterized by an R2 of 0.95 and an RMSEP of 0.06 g.L-1. For glycerin, a highly accurate PLSR model with an R2 of 0.98 and an RMSEP of 0.116 g.L-1 was obtained. These results indicate that an in situ NIRS probe is suitable for real-time monitoring of important parameters in alcoholic fermentations. |
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Brazilian Journal of Chemical Engineering |
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Real time and in situ Near-Infrared Spectroscopy (Nirs) for Quantitative Monitoring of Biomass, Glucose, Ethanol and Glycerine concentrations in an alcoholic fermentationnear-infrared spectroscopymonitoringalcoholic fermentationAbstract This study investigates the application of NIR spectroscopy for real-time monitoring of process parameters in alcoholic fermentations. For this purpose, fermentation batches using Saccharomyces cerevisiae were carried out and monitored in situ by an immersed NIRS probe. Samples were collected throughout the fermentations, and concentrations of biomass, glucose, ethanol and glycerin were analyzed by reference methods and multivariate data analyses such as PCA and PLSR. PCA was used to investigate data variability and to select samples for calibration and for external validation sets. A PLSR model for biomass yielded R2 and RMSEP values of 0.99 and 0.276 g.L-1, respectively. For glucose, the carbon source, the PLSR model yielded an R2 of 0.95 and an RMSEP of 7.14 g.L-1. The PLSR model for ethanol was characterized by an R2 of 0.95 and an RMSEP of 0.06 g.L-1. For glycerin, a highly accurate PLSR model with an R2 of 0.98 and an RMSEP of 0.116 g.L-1 was obtained. These results indicate that an in situ NIRS probe is suitable for real-time monitoring of important parameters in alcoholic fermentations.Brazilian Society of Chemical Engineering2017-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322017000200459Brazilian Journal of Chemical Engineering v.34 n.2 2017reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/0104-6632.20170342s20150347info:eu-repo/semantics/openAccessNascimento,Ruthinéia Jéssica Alves doMacedo,Gorete Ribeiro deSantos,Everaldo Silvino dosOliveira,Jackson Araújo deeng2017-10-05T00:00:00Zoai:scielo:S0104-66322017000200459Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2017-10-05T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
Real time and in situ Near-Infrared Spectroscopy (Nirs) for Quantitative Monitoring of Biomass, Glucose, Ethanol and Glycerine concentrations in an alcoholic fermentation |
title |
Real time and in situ Near-Infrared Spectroscopy (Nirs) for Quantitative Monitoring of Biomass, Glucose, Ethanol and Glycerine concentrations in an alcoholic fermentation |
spellingShingle |
Real time and in situ Near-Infrared Spectroscopy (Nirs) for Quantitative Monitoring of Biomass, Glucose, Ethanol and Glycerine concentrations in an alcoholic fermentation Nascimento,Ruthinéia Jéssica Alves do near-infrared spectroscopy monitoring alcoholic fermentation |
title_short |
Real time and in situ Near-Infrared Spectroscopy (Nirs) for Quantitative Monitoring of Biomass, Glucose, Ethanol and Glycerine concentrations in an alcoholic fermentation |
title_full |
Real time and in situ Near-Infrared Spectroscopy (Nirs) for Quantitative Monitoring of Biomass, Glucose, Ethanol and Glycerine concentrations in an alcoholic fermentation |
title_fullStr |
Real time and in situ Near-Infrared Spectroscopy (Nirs) for Quantitative Monitoring of Biomass, Glucose, Ethanol and Glycerine concentrations in an alcoholic fermentation |
title_full_unstemmed |
Real time and in situ Near-Infrared Spectroscopy (Nirs) for Quantitative Monitoring of Biomass, Glucose, Ethanol and Glycerine concentrations in an alcoholic fermentation |
title_sort |
Real time and in situ Near-Infrared Spectroscopy (Nirs) for Quantitative Monitoring of Biomass, Glucose, Ethanol and Glycerine concentrations in an alcoholic fermentation |
author |
Nascimento,Ruthinéia Jéssica Alves do |
author_facet |
Nascimento,Ruthinéia Jéssica Alves do Macedo,Gorete Ribeiro de Santos,Everaldo Silvino dos Oliveira,Jackson Araújo de |
author_role |
author |
author2 |
Macedo,Gorete Ribeiro de Santos,Everaldo Silvino dos Oliveira,Jackson Araújo de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Nascimento,Ruthinéia Jéssica Alves do Macedo,Gorete Ribeiro de Santos,Everaldo Silvino dos Oliveira,Jackson Araújo de |
dc.subject.por.fl_str_mv |
near-infrared spectroscopy monitoring alcoholic fermentation |
topic |
near-infrared spectroscopy monitoring alcoholic fermentation |
description |
Abstract This study investigates the application of NIR spectroscopy for real-time monitoring of process parameters in alcoholic fermentations. For this purpose, fermentation batches using Saccharomyces cerevisiae were carried out and monitored in situ by an immersed NIRS probe. Samples were collected throughout the fermentations, and concentrations of biomass, glucose, ethanol and glycerin were analyzed by reference methods and multivariate data analyses such as PCA and PLSR. PCA was used to investigate data variability and to select samples for calibration and for external validation sets. A PLSR model for biomass yielded R2 and RMSEP values of 0.99 and 0.276 g.L-1, respectively. For glucose, the carbon source, the PLSR model yielded an R2 of 0.95 and an RMSEP of 7.14 g.L-1. The PLSR model for ethanol was characterized by an R2 of 0.95 and an RMSEP of 0.06 g.L-1. For glycerin, a highly accurate PLSR model with an R2 of 0.98 and an RMSEP of 0.116 g.L-1 was obtained. These results indicate that an in situ NIRS probe is suitable for real-time monitoring of important parameters in alcoholic fermentations. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322017000200459 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322017000200459 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0104-6632.20170342s20150347 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.34 n.2 2017 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213175496015872 |