Selective media for detection and quantification of Brazilian Ralstonia solanacearum isolates in soil

Detalhes bibliográficos
Autor(a) principal: Pontes,Nadson C
Data de Publicação: 2017
Outros Autores: Fujinawa,Miriam F, Oliveira,José R
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Horticultura Brasileira
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362017000100041
Resumo: ABSTRACT The efficacy of five selective media described in the literature for the detection of Ralstonia solanacearum in soil was evaluated using isolates from different regions of Brazil. The selective media were evaluated regarding their sensitivity, target-pathogen repression index, capacity for suppressing contaminating microorganisms and recovery rate of the pathogen in soil. No bacterial growth was observed at the concentrations tested for FSM and SM-1 media. Some isolates grew on the PCCG medium with the addition of antimicrobial agents, but only at concentrations of 106 cfu/mL. For the SM and SMSA-E media, adequate sensitivity for the detection of R. solanacearum in soil was observed, enabling detection at a concentration of approximately 103 cfu/gram of soil. SMSA-E (Selective Medium South Africa - Elphinstone) was found to be the most effective in suppressing the growth of contaminating microorganisms, resulting in the lowest rate of repression of R. solanacearum and the highest recovery rate of the bacterium in the soil.
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spelling Selective media for detection and quantification of Brazilian Ralstonia solanacearum isolates in soilbacterial wiltculture mediumdiagnosisSMSA-E.ABSTRACT The efficacy of five selective media described in the literature for the detection of Ralstonia solanacearum in soil was evaluated using isolates from different regions of Brazil. The selective media were evaluated regarding their sensitivity, target-pathogen repression index, capacity for suppressing contaminating microorganisms and recovery rate of the pathogen in soil. No bacterial growth was observed at the concentrations tested for FSM and SM-1 media. Some isolates grew on the PCCG medium with the addition of antimicrobial agents, but only at concentrations of 106 cfu/mL. For the SM and SMSA-E media, adequate sensitivity for the detection of R. solanacearum in soil was observed, enabling detection at a concentration of approximately 103 cfu/gram of soil. SMSA-E (Selective Medium South Africa - Elphinstone) was found to be the most effective in suppressing the growth of contaminating microorganisms, resulting in the lowest rate of repression of R. solanacearum and the highest recovery rate of the bacterium in the soil.Associação Brasileira de Horticultura2017-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362017000100041Horticultura Brasileira v.35 n.1 2017reponame:Horticultura Brasileirainstname:Associação Brasileira de Horticultura (ABH)instacron:ABH10.1590/s0102-053620170107info:eu-repo/semantics/openAccessPontes,Nadson CFujinawa,Miriam FOliveira,José Reng2017-04-17T00:00:00Zoai:scielo:S0102-05362017000100041Revistahttp://cms.horticulturabrasileira.com.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||hortbras@gmail.com1806-99910102-0536opendoar:2017-04-17T00:00Horticultura Brasileira - Associação Brasileira de Horticultura (ABH)false
dc.title.none.fl_str_mv Selective media for detection and quantification of Brazilian Ralstonia solanacearum isolates in soil
title Selective media for detection and quantification of Brazilian Ralstonia solanacearum isolates in soil
spellingShingle Selective media for detection and quantification of Brazilian Ralstonia solanacearum isolates in soil
Pontes,Nadson C
bacterial wilt
culture medium
diagnosis
SMSA-E.
title_short Selective media for detection and quantification of Brazilian Ralstonia solanacearum isolates in soil
title_full Selective media for detection and quantification of Brazilian Ralstonia solanacearum isolates in soil
title_fullStr Selective media for detection and quantification of Brazilian Ralstonia solanacearum isolates in soil
title_full_unstemmed Selective media for detection and quantification of Brazilian Ralstonia solanacearum isolates in soil
title_sort Selective media for detection and quantification of Brazilian Ralstonia solanacearum isolates in soil
author Pontes,Nadson C
author_facet Pontes,Nadson C
Fujinawa,Miriam F
Oliveira,José R
author_role author
author2 Fujinawa,Miriam F
Oliveira,José R
author2_role author
author
dc.contributor.author.fl_str_mv Pontes,Nadson C
Fujinawa,Miriam F
Oliveira,José R
dc.subject.por.fl_str_mv bacterial wilt
culture medium
diagnosis
SMSA-E.
topic bacterial wilt
culture medium
diagnosis
SMSA-E.
description ABSTRACT The efficacy of five selective media described in the literature for the detection of Ralstonia solanacearum in soil was evaluated using isolates from different regions of Brazil. The selective media were evaluated regarding their sensitivity, target-pathogen repression index, capacity for suppressing contaminating microorganisms and recovery rate of the pathogen in soil. No bacterial growth was observed at the concentrations tested for FSM and SM-1 media. Some isolates grew on the PCCG medium with the addition of antimicrobial agents, but only at concentrations of 106 cfu/mL. For the SM and SMSA-E media, adequate sensitivity for the detection of R. solanacearum in soil was observed, enabling detection at a concentration of approximately 103 cfu/gram of soil. SMSA-E (Selective Medium South Africa - Elphinstone) was found to be the most effective in suppressing the growth of contaminating microorganisms, resulting in the lowest rate of repression of R. solanacearum and the highest recovery rate of the bacterium in the soil.
publishDate 2017
dc.date.none.fl_str_mv 2017-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362017000100041
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362017000100041
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s0102-053620170107
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Horticultura
publisher.none.fl_str_mv Associação Brasileira de Horticultura
dc.source.none.fl_str_mv Horticultura Brasileira v.35 n.1 2017
reponame:Horticultura Brasileira
instname:Associação Brasileira de Horticultura (ABH)
instacron:ABH
instname_str Associação Brasileira de Horticultura (ABH)
instacron_str ABH
institution ABH
reponame_str Horticultura Brasileira
collection Horticultura Brasileira
repository.name.fl_str_mv Horticultura Brasileira - Associação Brasileira de Horticultura (ABH)
repository.mail.fl_str_mv ||hortbras@gmail.com
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