In vitro bioactivity and cytotoxicity of chemically treated glass fibers

Detalhes bibliográficos
Autor(a) principal: Andrade,Ângela Leão
Data de Publicação: 2004
Outros Autores: Turchetti-Maia,Regina Maria de Marco, Lopes,Míriam Teresa Paz, Salas,Carlos Edmundo, Domingues,Rosana Zacarias
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Materials research (São Carlos. Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392004000400019
Resumo: Samples of a commercial glass fiber FM® (Fiber Max) were used to test the efficacy of a chemical sol-gel surface treatment to enhance their bioactivity. After treatment with tetraethoxysilane (TEOS), individual fiber samples were soaked into a simulated body fluid (SBF) solution, from which they were removed at intervals of 5 and 10 days. Micrographs obtained by scanning electron microscopy (SEM) analysis of samples chemically treated with TEOS revealed the formation of a hydroxyapatite (HA) coating layer after 5 days into SBF solution. Fourier transform infrared spectroscopic (FTIR) analyses confirmed that the coating layer has P-O vibration bands characteristic of HA. The in vitro cytotoxicity was evaluated using a direct contact test, minimum essential medium elution test (ISO 10993-5) and MTT assay. Fibers immersed in SBF and their extracts exhibited lower cytotoxicity than the controls not subjected to immersion, suggesting that SBF treatment improves the biocompatibility of the fiber.
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spelling In vitro bioactivity and cytotoxicity of chemically treated glass fibersglass fiberchemical treatmentbioactivitycytotoxicitySamples of a commercial glass fiber FM® (Fiber Max) were used to test the efficacy of a chemical sol-gel surface treatment to enhance their bioactivity. After treatment with tetraethoxysilane (TEOS), individual fiber samples were soaked into a simulated body fluid (SBF) solution, from which they were removed at intervals of 5 and 10 days. Micrographs obtained by scanning electron microscopy (SEM) analysis of samples chemically treated with TEOS revealed the formation of a hydroxyapatite (HA) coating layer after 5 days into SBF solution. Fourier transform infrared spectroscopic (FTIR) analyses confirmed that the coating layer has P-O vibration bands characteristic of HA. The in vitro cytotoxicity was evaluated using a direct contact test, minimum essential medium elution test (ISO 10993-5) and MTT assay. Fibers immersed in SBF and their extracts exhibited lower cytotoxicity than the controls not subjected to immersion, suggesting that SBF treatment improves the biocompatibility of the fiber.ABM, ABC, ABPol2004-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392004000400019Materials Research v.7 n.4 2004reponame:Materials research (São Carlos. Online)instname:Universidade Federal de São Carlos (UFSCAR)instacron:ABM ABC ABPOL10.1590/S1516-14392004000400019info:eu-repo/semantics/openAccessAndrade,Ângela LeãoTurchetti-Maia,Regina Maria de MarcoLopes,Míriam Teresa PazSalas,Carlos EdmundoDomingues,Rosana Zacariaseng2005-11-08T00:00:00Zoai:scielo:S1516-14392004000400019Revistahttp://www.scielo.br/mrPUBhttps://old.scielo.br/oai/scielo-oai.phpdedz@power.ufscar.br1980-53731516-1439opendoar:2005-11-08T00:00Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR)false
dc.title.none.fl_str_mv In vitro bioactivity and cytotoxicity of chemically treated glass fibers
title In vitro bioactivity and cytotoxicity of chemically treated glass fibers
spellingShingle In vitro bioactivity and cytotoxicity of chemically treated glass fibers
Andrade,Ângela Leão
glass fiber
chemical treatment
bioactivity
cytotoxicity
title_short In vitro bioactivity and cytotoxicity of chemically treated glass fibers
title_full In vitro bioactivity and cytotoxicity of chemically treated glass fibers
title_fullStr In vitro bioactivity and cytotoxicity of chemically treated glass fibers
title_full_unstemmed In vitro bioactivity and cytotoxicity of chemically treated glass fibers
title_sort In vitro bioactivity and cytotoxicity of chemically treated glass fibers
author Andrade,Ângela Leão
author_facet Andrade,Ângela Leão
Turchetti-Maia,Regina Maria de Marco
Lopes,Míriam Teresa Paz
Salas,Carlos Edmundo
Domingues,Rosana Zacarias
author_role author
author2 Turchetti-Maia,Regina Maria de Marco
Lopes,Míriam Teresa Paz
Salas,Carlos Edmundo
Domingues,Rosana Zacarias
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Andrade,Ângela Leão
Turchetti-Maia,Regina Maria de Marco
Lopes,Míriam Teresa Paz
Salas,Carlos Edmundo
Domingues,Rosana Zacarias
dc.subject.por.fl_str_mv glass fiber
chemical treatment
bioactivity
cytotoxicity
topic glass fiber
chemical treatment
bioactivity
cytotoxicity
description Samples of a commercial glass fiber FM® (Fiber Max) were used to test the efficacy of a chemical sol-gel surface treatment to enhance their bioactivity. After treatment with tetraethoxysilane (TEOS), individual fiber samples were soaked into a simulated body fluid (SBF) solution, from which they were removed at intervals of 5 and 10 days. Micrographs obtained by scanning electron microscopy (SEM) analysis of samples chemically treated with TEOS revealed the formation of a hydroxyapatite (HA) coating layer after 5 days into SBF solution. Fourier transform infrared spectroscopic (FTIR) analyses confirmed that the coating layer has P-O vibration bands characteristic of HA. The in vitro cytotoxicity was evaluated using a direct contact test, minimum essential medium elution test (ISO 10993-5) and MTT assay. Fibers immersed in SBF and their extracts exhibited lower cytotoxicity than the controls not subjected to immersion, suggesting that SBF treatment improves the biocompatibility of the fiber.
publishDate 2004
dc.date.none.fl_str_mv 2004-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392004000400019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392004000400019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-14392004000400019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv ABM, ABC, ABPol
publisher.none.fl_str_mv ABM, ABC, ABPol
dc.source.none.fl_str_mv Materials Research v.7 n.4 2004
reponame:Materials research (São Carlos. Online)
instname:Universidade Federal de São Carlos (UFSCAR)
instacron:ABM ABC ABPOL
instname_str Universidade Federal de São Carlos (UFSCAR)
instacron_str ABM ABC ABPOL
institution ABM ABC ABPOL
reponame_str Materials research (São Carlos. Online)
collection Materials research (São Carlos. Online)
repository.name.fl_str_mv Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR)
repository.mail.fl_str_mv dedz@power.ufscar.br
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