The Effect of Processing Parameters and Solid Concentration on the Microstructure and Pore Architecture of Gelatin-Chitosan Scaffolds Produced by Freeze-Drying
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Materials research (São Carlos. Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392016000400839 |
Resumo: | One of the main components for being successful in tissue engineering is developing a scaffold with an appropriate architecture for allow migration, cell proliferation, and differentiation. A gelatin-chitosan scaffold by vacuum freeze-drying has been developed for tissue engineering applications. The effects of solid concentration and freezing processing on the scaffold morphology and porous size were investigated. As the chitosan content was increased the viscoelastic properties of pigskin gelatin was modified, the maximum G' values were lower than the values for pure gelatin solution, and the thermal transition points also occurred at lower temperatures, as well as a decrease of pore size tendency was observed and the scaffold visibly increased porosity, the structure scaffold was observed with an interconnected and more homogeneous pore matrix. The pore sizes become smaller and pore walls thinner, while interconnectivity increases along with declining pre-freezing temperature. The chitosan-gelatin scaffold will be a promising candidate in tissue engineering. |
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Materials research (São Carlos. Online) |
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The Effect of Processing Parameters and Solid Concentration on the Microstructure and Pore Architecture of Gelatin-Chitosan Scaffolds Produced by Freeze-Dryingtissue engineeringcell proliferationinterconnected pore matrixOne of the main components for being successful in tissue engineering is developing a scaffold with an appropriate architecture for allow migration, cell proliferation, and differentiation. A gelatin-chitosan scaffold by vacuum freeze-drying has been developed for tissue engineering applications. The effects of solid concentration and freezing processing on the scaffold morphology and porous size were investigated. As the chitosan content was increased the viscoelastic properties of pigskin gelatin was modified, the maximum G' values were lower than the values for pure gelatin solution, and the thermal transition points also occurred at lower temperatures, as well as a decrease of pore size tendency was observed and the scaffold visibly increased porosity, the structure scaffold was observed with an interconnected and more homogeneous pore matrix. The pore sizes become smaller and pore walls thinner, while interconnectivity increases along with declining pre-freezing temperature. The chitosan-gelatin scaffold will be a promising candidate in tissue engineering.ABM, ABC, ABPol2016-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392016000400839Materials Research v.19 n.4 2016reponame:Materials research (São Carlos. Online)instname:Universidade Federal de São Carlos (UFSCAR)instacron:ABM ABC ABPOL10.1590/1980-5373-MR-2015-0793info:eu-repo/semantics/openAccessAngulo,Daniel Enrique LópezSobral,Paulo José do Amaraleng2016-09-16T00:00:00Zoai:scielo:S1516-14392016000400839Revistahttp://www.scielo.br/mrPUBhttps://old.scielo.br/oai/scielo-oai.phpdedz@power.ufscar.br1980-53731516-1439opendoar:2016-09-16T00:00Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.none.fl_str_mv |
The Effect of Processing Parameters and Solid Concentration on the Microstructure and Pore Architecture of Gelatin-Chitosan Scaffolds Produced by Freeze-Drying |
title |
The Effect of Processing Parameters and Solid Concentration on the Microstructure and Pore Architecture of Gelatin-Chitosan Scaffolds Produced by Freeze-Drying |
spellingShingle |
The Effect of Processing Parameters and Solid Concentration on the Microstructure and Pore Architecture of Gelatin-Chitosan Scaffolds Produced by Freeze-Drying Angulo,Daniel Enrique López tissue engineering cell proliferation interconnected pore matrix |
title_short |
The Effect of Processing Parameters and Solid Concentration on the Microstructure and Pore Architecture of Gelatin-Chitosan Scaffolds Produced by Freeze-Drying |
title_full |
The Effect of Processing Parameters and Solid Concentration on the Microstructure and Pore Architecture of Gelatin-Chitosan Scaffolds Produced by Freeze-Drying |
title_fullStr |
The Effect of Processing Parameters and Solid Concentration on the Microstructure and Pore Architecture of Gelatin-Chitosan Scaffolds Produced by Freeze-Drying |
title_full_unstemmed |
The Effect of Processing Parameters and Solid Concentration on the Microstructure and Pore Architecture of Gelatin-Chitosan Scaffolds Produced by Freeze-Drying |
title_sort |
The Effect of Processing Parameters and Solid Concentration on the Microstructure and Pore Architecture of Gelatin-Chitosan Scaffolds Produced by Freeze-Drying |
author |
Angulo,Daniel Enrique López |
author_facet |
Angulo,Daniel Enrique López Sobral,Paulo José do Amaral |
author_role |
author |
author2 |
Sobral,Paulo José do Amaral |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Angulo,Daniel Enrique López Sobral,Paulo José do Amaral |
dc.subject.por.fl_str_mv |
tissue engineering cell proliferation interconnected pore matrix |
topic |
tissue engineering cell proliferation interconnected pore matrix |
description |
One of the main components for being successful in tissue engineering is developing a scaffold with an appropriate architecture for allow migration, cell proliferation, and differentiation. A gelatin-chitosan scaffold by vacuum freeze-drying has been developed for tissue engineering applications. The effects of solid concentration and freezing processing on the scaffold morphology and porous size were investigated. As the chitosan content was increased the viscoelastic properties of pigskin gelatin was modified, the maximum G' values were lower than the values for pure gelatin solution, and the thermal transition points also occurred at lower temperatures, as well as a decrease of pore size tendency was observed and the scaffold visibly increased porosity, the structure scaffold was observed with an interconnected and more homogeneous pore matrix. The pore sizes become smaller and pore walls thinner, while interconnectivity increases along with declining pre-freezing temperature. The chitosan-gelatin scaffold will be a promising candidate in tissue engineering. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392016000400839 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392016000400839 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1980-5373-MR-2015-0793 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
ABM, ABC, ABPol |
publisher.none.fl_str_mv |
ABM, ABC, ABPol |
dc.source.none.fl_str_mv |
Materials Research v.19 n.4 2016 reponame:Materials research (São Carlos. Online) instname:Universidade Federal de São Carlos (UFSCAR) instacron:ABM ABC ABPOL |
instname_str |
Universidade Federal de São Carlos (UFSCAR) |
instacron_str |
ABM ABC ABPOL |
institution |
ABM ABC ABPOL |
reponame_str |
Materials research (São Carlos. Online) |
collection |
Materials research (São Carlos. Online) |
repository.name.fl_str_mv |
Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR) |
repository.mail.fl_str_mv |
dedz@power.ufscar.br |
_version_ |
1754212667983134720 |