Low potential stable glucose detection at dendrimers modified polyaniline nanotubes
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Materials research (São Carlos. Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392010000100003 |
Resumo: | The utilization of nanostructured materials for development of biosensors is a growing field in medical diagnostics. In this work a glucose biosensor based on bioactive polyglycerol (PGLD) and chitosan dendrimers (CHD) was developed. PGLD and CHD were bioconjugated with the enzyme glucose oxidase (GOx) to obtain dendrimers with glucose sensing properties. Polyaniline nanotubes (PANINT´s) were used as electron mediator due to their high ability to promote electron-transfer reactions involving GOx. The PGLD-GOx and CHD-GOx were entrapped in PANINT´s during template electrochemical polymerization of aniline. The prepared PGLD-GOx/PANINT´s and CHD-GOx/PANINT´s biosensors exhibit a strong and stable amperometric response to glucose even at a low potential of +100 mV. The based PGLD-GOx/PANINT´s and CHD-GOx/PANINT´s biosensors showed a good performance in glucose concentrations range in human blood. A comparison of the sensitivities to glucose showed that both biosensors have a linearity range between 0.02 and 10 mM, though PGLD-GOx/PANINT´s is more sensitive (10.41 vs. 7.04 nA.mM-1). The difference in the biosensor behavior and the high sensitivity of the PGLD-GOx/PANINT´s may be due to the specific organization of GOx layer at surface of the modifier macromolecule PGLD and their distribution in PANINT´s. The enzyme affinity for the substrate, K Mapp remains quite good after GOx immobilization on PGLD and CHD dendrimers and entrapment of the bioconjugates in PANINT´s. |
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Materials research (São Carlos. Online) |
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Low potential stable glucose detection at dendrimers modified polyaniline nanotubespolyglycerol dendrimerschitosan dendrimerspolyaniline nanotubesglucose biosensorThe utilization of nanostructured materials for development of biosensors is a growing field in medical diagnostics. In this work a glucose biosensor based on bioactive polyglycerol (PGLD) and chitosan dendrimers (CHD) was developed. PGLD and CHD were bioconjugated with the enzyme glucose oxidase (GOx) to obtain dendrimers with glucose sensing properties. Polyaniline nanotubes (PANINT´s) were used as electron mediator due to their high ability to promote electron-transfer reactions involving GOx. The PGLD-GOx and CHD-GOx were entrapped in PANINT´s during template electrochemical polymerization of aniline. The prepared PGLD-GOx/PANINT´s and CHD-GOx/PANINT´s biosensors exhibit a strong and stable amperometric response to glucose even at a low potential of +100 mV. The based PGLD-GOx/PANINT´s and CHD-GOx/PANINT´s biosensors showed a good performance in glucose concentrations range in human blood. A comparison of the sensitivities to glucose showed that both biosensors have a linearity range between 0.02 and 10 mM, though PGLD-GOx/PANINT´s is more sensitive (10.41 vs. 7.04 nA.mM-1). The difference in the biosensor behavior and the high sensitivity of the PGLD-GOx/PANINT´s may be due to the specific organization of GOx layer at surface of the modifier macromolecule PGLD and their distribution in PANINT´s. The enzyme affinity for the substrate, K Mapp remains quite good after GOx immobilization on PGLD and CHD dendrimers and entrapment of the bioconjugates in PANINT´s.ABM, ABC, ABPol2010-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392010000100003Materials Research v.13 n.1 2010reponame:Materials research (São Carlos. Online)instname:Universidade Federal de São Carlos (UFSCAR)instacron:ABM ABC ABPOL10.1590/S1516-14392010000100003info:eu-repo/semantics/openAccessSantos,Alessandra NogueiraSoares,Demétrio Artur WernerQueiroz,Alvaro Antonio Alencar deeng2010-04-13T00:00:00Zoai:scielo:S1516-14392010000100003Revistahttp://www.scielo.br/mrPUBhttps://old.scielo.br/oai/scielo-oai.phpdedz@power.ufscar.br1980-53731516-1439opendoar:2010-04-13T00:00Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.none.fl_str_mv |
Low potential stable glucose detection at dendrimers modified polyaniline nanotubes |
title |
Low potential stable glucose detection at dendrimers modified polyaniline nanotubes |
spellingShingle |
Low potential stable glucose detection at dendrimers modified polyaniline nanotubes Santos,Alessandra Nogueira polyglycerol dendrimers chitosan dendrimers polyaniline nanotubes glucose biosensor |
title_short |
Low potential stable glucose detection at dendrimers modified polyaniline nanotubes |
title_full |
Low potential stable glucose detection at dendrimers modified polyaniline nanotubes |
title_fullStr |
Low potential stable glucose detection at dendrimers modified polyaniline nanotubes |
title_full_unstemmed |
Low potential stable glucose detection at dendrimers modified polyaniline nanotubes |
title_sort |
Low potential stable glucose detection at dendrimers modified polyaniline nanotubes |
author |
Santos,Alessandra Nogueira |
author_facet |
Santos,Alessandra Nogueira Soares,Demétrio Artur Werner Queiroz,Alvaro Antonio Alencar de |
author_role |
author |
author2 |
Soares,Demétrio Artur Werner Queiroz,Alvaro Antonio Alencar de |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Santos,Alessandra Nogueira Soares,Demétrio Artur Werner Queiroz,Alvaro Antonio Alencar de |
dc.subject.por.fl_str_mv |
polyglycerol dendrimers chitosan dendrimers polyaniline nanotubes glucose biosensor |
topic |
polyglycerol dendrimers chitosan dendrimers polyaniline nanotubes glucose biosensor |
description |
The utilization of nanostructured materials for development of biosensors is a growing field in medical diagnostics. In this work a glucose biosensor based on bioactive polyglycerol (PGLD) and chitosan dendrimers (CHD) was developed. PGLD and CHD were bioconjugated with the enzyme glucose oxidase (GOx) to obtain dendrimers with glucose sensing properties. Polyaniline nanotubes (PANINT´s) were used as electron mediator due to their high ability to promote electron-transfer reactions involving GOx. The PGLD-GOx and CHD-GOx were entrapped in PANINT´s during template electrochemical polymerization of aniline. The prepared PGLD-GOx/PANINT´s and CHD-GOx/PANINT´s biosensors exhibit a strong and stable amperometric response to glucose even at a low potential of +100 mV. The based PGLD-GOx/PANINT´s and CHD-GOx/PANINT´s biosensors showed a good performance in glucose concentrations range in human blood. A comparison of the sensitivities to glucose showed that both biosensors have a linearity range between 0.02 and 10 mM, though PGLD-GOx/PANINT´s is more sensitive (10.41 vs. 7.04 nA.mM-1). The difference in the biosensor behavior and the high sensitivity of the PGLD-GOx/PANINT´s may be due to the specific organization of GOx layer at surface of the modifier macromolecule PGLD and their distribution in PANINT´s. The enzyme affinity for the substrate, K Mapp remains quite good after GOx immobilization on PGLD and CHD dendrimers and entrapment of the bioconjugates in PANINT´s. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392010000100003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392010000100003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-14392010000100003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
ABM, ABC, ABPol |
publisher.none.fl_str_mv |
ABM, ABC, ABPol |
dc.source.none.fl_str_mv |
Materials Research v.13 n.1 2010 reponame:Materials research (São Carlos. Online) instname:Universidade Federal de São Carlos (UFSCAR) instacron:ABM ABC ABPOL |
instname_str |
Universidade Federal de São Carlos (UFSCAR) |
instacron_str |
ABM ABC ABPOL |
institution |
ABM ABC ABPOL |
reponame_str |
Materials research (São Carlos. Online) |
collection |
Materials research (São Carlos. Online) |
repository.name.fl_str_mv |
Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR) |
repository.mail.fl_str_mv |
dedz@power.ufscar.br |
_version_ |
1754212659349159936 |