Preparation of berbamine loaded chitosan-agarose microspheres and in vitro release study

Detalhes bibliográficos
Autor(a) principal: Hu,Zhang
Data de Publicação: 2012
Outros Autores: Li,Sidong, Yang,Lei
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Polímeros (São Carlos. Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282012000500004
Resumo: Berbamine loaded chitosan-agarose microspheres were prepared using a water-in-oil emulsion technique. Optimum preparing parameters were determined by orthogonal experiments as follows: ratio of berbamine to chitosan (w/w) is 1:10; percentage of emulsifier (span 80, v/v) is 6%; volume of glutaraldehyde is 2 mL; and reaction temperature is 70 ºC. Under these optimal conditions, the encapsulation efficiency and loading capacity of microspheres are 84.57% and 8.44%, respectively. The swelling tests showed that the microspheres possessed higher swelling ratio at pH 7.4 than at pH 1.2. FTIR indicated that berbamine had been successfully loaded in the chitosan-agarose microspheres by physical entrapment. In vitro release studies showed that berbamine was released from microspheres in a significantly sustained fashion.
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spelling Preparation of berbamine loaded chitosan-agarose microspheres and in vitro release studyChitosanagarosemicrospheresberbamineBerbamine loaded chitosan-agarose microspheres were prepared using a water-in-oil emulsion technique. Optimum preparing parameters were determined by orthogonal experiments as follows: ratio of berbamine to chitosan (w/w) is 1:10; percentage of emulsifier (span 80, v/v) is 6%; volume of glutaraldehyde is 2 mL; and reaction temperature is 70 ºC. Under these optimal conditions, the encapsulation efficiency and loading capacity of microspheres are 84.57% and 8.44%, respectively. The swelling tests showed that the microspheres possessed higher swelling ratio at pH 7.4 than at pH 1.2. FTIR indicated that berbamine had been successfully loaded in the chitosan-agarose microspheres by physical entrapment. In vitro release studies showed that berbamine was released from microspheres in a significantly sustained fashion.Associação Brasileira de Polímeros2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282012000500004Polímeros v.22 n.5 2012reponame:Polímeros (São Carlos. Online)instname:Associação Brasileira de Polímeros (ABPol)instacron:ABPO10.1590/S0104-14282012005000073info:eu-repo/semantics/openAccessHu,ZhangLi,SidongYang,Leieng2013-01-17T00:00:00Zoai:scielo:S0104-14282012000500004Revistahttp://www.scielo.br/pohttps://old.scielo.br/oai/scielo-oai.php||revista@abpol.org.br1678-51690104-1428opendoar:2013-01-17T00:00Polímeros (São Carlos. Online) - Associação Brasileira de Polímeros (ABPol)false
dc.title.none.fl_str_mv Preparation of berbamine loaded chitosan-agarose microspheres and in vitro release study
title Preparation of berbamine loaded chitosan-agarose microspheres and in vitro release study
spellingShingle Preparation of berbamine loaded chitosan-agarose microspheres and in vitro release study
Hu,Zhang
Chitosan
agarose
microspheres
berbamine
title_short Preparation of berbamine loaded chitosan-agarose microspheres and in vitro release study
title_full Preparation of berbamine loaded chitosan-agarose microspheres and in vitro release study
title_fullStr Preparation of berbamine loaded chitosan-agarose microspheres and in vitro release study
title_full_unstemmed Preparation of berbamine loaded chitosan-agarose microspheres and in vitro release study
title_sort Preparation of berbamine loaded chitosan-agarose microspheres and in vitro release study
author Hu,Zhang
author_facet Hu,Zhang
Li,Sidong
Yang,Lei
author_role author
author2 Li,Sidong
Yang,Lei
author2_role author
author
dc.contributor.author.fl_str_mv Hu,Zhang
Li,Sidong
Yang,Lei
dc.subject.por.fl_str_mv Chitosan
agarose
microspheres
berbamine
topic Chitosan
agarose
microspheres
berbamine
description Berbamine loaded chitosan-agarose microspheres were prepared using a water-in-oil emulsion technique. Optimum preparing parameters were determined by orthogonal experiments as follows: ratio of berbamine to chitosan (w/w) is 1:10; percentage of emulsifier (span 80, v/v) is 6%; volume of glutaraldehyde is 2 mL; and reaction temperature is 70 ºC. Under these optimal conditions, the encapsulation efficiency and loading capacity of microspheres are 84.57% and 8.44%, respectively. The swelling tests showed that the microspheres possessed higher swelling ratio at pH 7.4 than at pH 1.2. FTIR indicated that berbamine had been successfully loaded in the chitosan-agarose microspheres by physical entrapment. In vitro release studies showed that berbamine was released from microspheres in a significantly sustained fashion.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282012000500004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282012000500004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0104-14282012005000073
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Polímeros
publisher.none.fl_str_mv Associação Brasileira de Polímeros
dc.source.none.fl_str_mv Polímeros v.22 n.5 2012
reponame:Polímeros (São Carlos. Online)
instname:Associação Brasileira de Polímeros (ABPol)
instacron:ABPO
instname_str Associação Brasileira de Polímeros (ABPol)
instacron_str ABPO
institution ABPO
reponame_str Polímeros (São Carlos. Online)
collection Polímeros (São Carlos. Online)
repository.name.fl_str_mv Polímeros (São Carlos. Online) - Associação Brasileira de Polímeros (ABPol)
repository.mail.fl_str_mv ||revista@abpol.org.br
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