Inhibition of carrageenan-induced expression of tissue and plasma prekallikreins mRNA by low level laser therapy in a rat paw edema model
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Physical Therapy |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-35552011000100002 |
Resumo: | BACKGROND: Low level laser therapy (LLLT) has been used clinically in order to treat inflammation, where tissue and plasma prekallikrein have crucial importance. Plasma prekallikrein (PPK) is synthesized by the hepatocytes and secreted into the bloodstream, where it participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. Tissue prekallikrein is associated with important disease states (including cancer, inflammation, and neurodegeneration) and has been utilized or proposed as clinically important biomarker or therapeutic target of interest. OBJECTIVE: To evaluate if LLLT modulates tissue and plasma prekallikreins mRNA expression in the carrageenan-induced rat paw edema. METHODS: Experimental groups were assigned as followed: A1 (Control-saline), A2 (Carrageenan-only), A3 (laser 660nm only) and A4 (Carrageenan + laser 660nm). Edema was measured by a plethysmometer. Subplantar tissue was collected for the quantification of prekallikreins mRNA by Real time-Polymerase Chain Reaction. RESULTS: A significantly decrease in the edema was observed after laser irradiation. Expression of prekallikreins increased after carrageenan injection. Tissue and plasma prekallikrein mRNA expression significantly decreased after LLLT's 660nm wavelength. CONCLUSION: These results suggest that expression of tissue and plasma prekallikreins is modulated by LLLT, which can be used in clinical practice due to its anti-inflammatory effects. |
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Brazilian Journal of Physical Therapy |
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Inhibition of carrageenan-induced expression of tissue and plasma prekallikreins mRNA by low level laser therapy in a rat paw edema modelPlasma kallikreintissue kallikreinlow-level laser therapygene expressioninflammationBACKGROND: Low level laser therapy (LLLT) has been used clinically in order to treat inflammation, where tissue and plasma prekallikrein have crucial importance. Plasma prekallikrein (PPK) is synthesized by the hepatocytes and secreted into the bloodstream, where it participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. Tissue prekallikrein is associated with important disease states (including cancer, inflammation, and neurodegeneration) and has been utilized or proposed as clinically important biomarker or therapeutic target of interest. OBJECTIVE: To evaluate if LLLT modulates tissue and plasma prekallikreins mRNA expression in the carrageenan-induced rat paw edema. METHODS: Experimental groups were assigned as followed: A1 (Control-saline), A2 (Carrageenan-only), A3 (laser 660nm only) and A4 (Carrageenan + laser 660nm). Edema was measured by a plethysmometer. Subplantar tissue was collected for the quantification of prekallikreins mRNA by Real time-Polymerase Chain Reaction. RESULTS: A significantly decrease in the edema was observed after laser irradiation. Expression of prekallikreins increased after carrageenan injection. Tissue and plasma prekallikrein mRNA expression significantly decreased after LLLT's 660nm wavelength. CONCLUSION: These results suggest that expression of tissue and plasma prekallikreins is modulated by LLLT, which can be used in clinical practice due to its anti-inflammatory effects.Associação Brasileira de Pesquisa e Pós-Graduação em Fisioterapia 2011-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-35552011000100002Brazilian Journal of Physical Therapy v.15 n.1 2011reponame:Brazilian Journal of Physical Therapyinstname:Associação Brasileira de Pesquisa e Pós-Graduação em Fisioterapia (ABRAPG-FT)instacron:ABRAPG-FT10.1590/S1413-35552011005000005info:eu-repo/semantics/openAccessSilva,Moisés P.Bortone,FláviaSilva,Marcelo P.Araújo,Thiago R.Costa,Maricília S.Silva Júnior,José A.eng2011-05-03T00:00:00Zoai:scielo:S1413-35552011000100002Revistahttps://www.scielo.br/j/rbfis/https://old.scielo.br/oai/scielo-oai.phpcontato@rbf-bjpt.org.br||contato@rbf-bjpt.org.br1809-92461413-3555opendoar:2011-05-03T00:00Brazilian Journal of Physical Therapy - Associação Brasileira de Pesquisa e Pós-Graduação em Fisioterapia (ABRAPG-FT)false |
dc.title.none.fl_str_mv |
Inhibition of carrageenan-induced expression of tissue and plasma prekallikreins mRNA by low level laser therapy in a rat paw edema model |
title |
Inhibition of carrageenan-induced expression of tissue and plasma prekallikreins mRNA by low level laser therapy in a rat paw edema model |
spellingShingle |
Inhibition of carrageenan-induced expression of tissue and plasma prekallikreins mRNA by low level laser therapy in a rat paw edema model Silva,Moisés P. Plasma kallikrein tissue kallikrein low-level laser therapy gene expression inflammation |
title_short |
Inhibition of carrageenan-induced expression of tissue and plasma prekallikreins mRNA by low level laser therapy in a rat paw edema model |
title_full |
Inhibition of carrageenan-induced expression of tissue and plasma prekallikreins mRNA by low level laser therapy in a rat paw edema model |
title_fullStr |
Inhibition of carrageenan-induced expression of tissue and plasma prekallikreins mRNA by low level laser therapy in a rat paw edema model |
title_full_unstemmed |
Inhibition of carrageenan-induced expression of tissue and plasma prekallikreins mRNA by low level laser therapy in a rat paw edema model |
title_sort |
Inhibition of carrageenan-induced expression of tissue and plasma prekallikreins mRNA by low level laser therapy in a rat paw edema model |
author |
Silva,Moisés P. |
author_facet |
Silva,Moisés P. Bortone,Flávia Silva,Marcelo P. Araújo,Thiago R. Costa,Maricília S. Silva Júnior,José A. |
author_role |
author |
author2 |
Bortone,Flávia Silva,Marcelo P. Araújo,Thiago R. Costa,Maricília S. Silva Júnior,José A. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Silva,Moisés P. Bortone,Flávia Silva,Marcelo P. Araújo,Thiago R. Costa,Maricília S. Silva Júnior,José A. |
dc.subject.por.fl_str_mv |
Plasma kallikrein tissue kallikrein low-level laser therapy gene expression inflammation |
topic |
Plasma kallikrein tissue kallikrein low-level laser therapy gene expression inflammation |
description |
BACKGROND: Low level laser therapy (LLLT) has been used clinically in order to treat inflammation, where tissue and plasma prekallikrein have crucial importance. Plasma prekallikrein (PPK) is synthesized by the hepatocytes and secreted into the bloodstream, where it participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. Tissue prekallikrein is associated with important disease states (including cancer, inflammation, and neurodegeneration) and has been utilized or proposed as clinically important biomarker or therapeutic target of interest. OBJECTIVE: To evaluate if LLLT modulates tissue and plasma prekallikreins mRNA expression in the carrageenan-induced rat paw edema. METHODS: Experimental groups were assigned as followed: A1 (Control-saline), A2 (Carrageenan-only), A3 (laser 660nm only) and A4 (Carrageenan + laser 660nm). Edema was measured by a plethysmometer. Subplantar tissue was collected for the quantification of prekallikreins mRNA by Real time-Polymerase Chain Reaction. RESULTS: A significantly decrease in the edema was observed after laser irradiation. Expression of prekallikreins increased after carrageenan injection. Tissue and plasma prekallikrein mRNA expression significantly decreased after LLLT's 660nm wavelength. CONCLUSION: These results suggest that expression of tissue and plasma prekallikreins is modulated by LLLT, which can be used in clinical practice due to its anti-inflammatory effects. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-35552011000100002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-35552011000100002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1413-35552011005000005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Pesquisa e Pós-Graduação em Fisioterapia |
publisher.none.fl_str_mv |
Associação Brasileira de Pesquisa e Pós-Graduação em Fisioterapia |
dc.source.none.fl_str_mv |
Brazilian Journal of Physical Therapy v.15 n.1 2011 reponame:Brazilian Journal of Physical Therapy instname:Associação Brasileira de Pesquisa e Pós-Graduação em Fisioterapia (ABRAPG-FT) instacron:ABRAPG-FT |
instname_str |
Associação Brasileira de Pesquisa e Pós-Graduação em Fisioterapia (ABRAPG-FT) |
instacron_str |
ABRAPG-FT |
institution |
ABRAPG-FT |
reponame_str |
Brazilian Journal of Physical Therapy |
collection |
Brazilian Journal of Physical Therapy |
repository.name.fl_str_mv |
Brazilian Journal of Physical Therapy - Associação Brasileira de Pesquisa e Pós-Graduação em Fisioterapia (ABRAPG-FT) |
repository.mail.fl_str_mv |
contato@rbf-bjpt.org.br||contato@rbf-bjpt.org.br |
_version_ |
1754575948702810112 |