p53 gene analysis in childhood B non - Hodgkin's lymphoma
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | São Paulo medical journal (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802001000600006 |
Resumo: | CONTEXT: Mutations or deletions in the tumor-suppressor gene p53 are among the commonest genetic changes found in human neoplasms including breast, lung and bowel cancers. In hematological malignancies, p53 is most often mutated in Burkitt's lymphoma, with p53 mutations present in 30 to 40% of tumor samples and in 70% of cell lines. OBJECTIVE: To analyze the p53 gene alterations in child patients with B non-Hodgkin's lymphoma. DESIGN: Descriptive study. SETTING: Tertiary oncology care center. PARTICIPANTS: The study investigated 12 patients with childhood B non-Hodgkin's lymphoma (Burkitt's lymphoma). Screening for p53 mutations was done by polymerase chain reaction - single strand conformational polymorphism (PCR-SSCP) analysis of exon 5 to 8/9 of the gene. RESULTS: Abnormal polymerase chain reaction - single strand conformational polymorphism migration pattern was observed in 4 patients (33.3%), one on exon 6 and three on exon 7. Positive cases included 2 patients who died from disease. CONCLUSION: These preliminary results suggest that p53 mutations are quite frequent in children with Burkitt's lymphoma and may play a role in lymphoma genesis or disease progression. |
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São Paulo medical journal (Online) |
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p53 gene analysis in childhood B non - Hodgkin's lymphomap53 mutationB non-Hodgkin's lymphomaBurkitt's lymphomaCONTEXT: Mutations or deletions in the tumor-suppressor gene p53 are among the commonest genetic changes found in human neoplasms including breast, lung and bowel cancers. In hematological malignancies, p53 is most often mutated in Burkitt's lymphoma, with p53 mutations present in 30 to 40% of tumor samples and in 70% of cell lines. OBJECTIVE: To analyze the p53 gene alterations in child patients with B non-Hodgkin's lymphoma. DESIGN: Descriptive study. SETTING: Tertiary oncology care center. PARTICIPANTS: The study investigated 12 patients with childhood B non-Hodgkin's lymphoma (Burkitt's lymphoma). Screening for p53 mutations was done by polymerase chain reaction - single strand conformational polymorphism (PCR-SSCP) analysis of exon 5 to 8/9 of the gene. RESULTS: Abnormal polymerase chain reaction - single strand conformational polymorphism migration pattern was observed in 4 patients (33.3%), one on exon 6 and three on exon 7. Positive cases included 2 patients who died from disease. CONCLUSION: These preliminary results suggest that p53 mutations are quite frequent in children with Burkitt's lymphoma and may play a role in lymphoma genesis or disease progression.Associação Paulista de Medicina - APM2001-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802001000600006Sao Paulo Medical Journal v.119 n.6 2001reponame:São Paulo medical journal (Online)instname:Associação Paulista de Medicinainstacron:APM10.1590/S1516-31802001000600006info:eu-repo/semantics/openAccessKlumb,Claudete Esteves Nogueira PintoResende,Lídia Maria Magalhães deTajara,Eloísa HelenaBertelli,Erika Cristina PavarinoRumjanek,Vivian MaryMaia,Raquel Ciuvalschieng2001-11-27T00:00:00Zoai:scielo:S1516-31802001000600006Revistahttp://www.scielo.br/spmjhttps://old.scielo.br/oai/scielo-oai.phprevistas@apm.org.br1806-94601516-3180opendoar:2001-11-27T00:00São Paulo medical journal (Online) - Associação Paulista de Medicinafalse |
dc.title.none.fl_str_mv |
p53 gene analysis in childhood B non - Hodgkin's lymphoma |
title |
p53 gene analysis in childhood B non - Hodgkin's lymphoma |
spellingShingle |
p53 gene analysis in childhood B non - Hodgkin's lymphoma Klumb,Claudete Esteves Nogueira Pinto p53 mutation B non-Hodgkin's lymphoma Burkitt's lymphoma |
title_short |
p53 gene analysis in childhood B non - Hodgkin's lymphoma |
title_full |
p53 gene analysis in childhood B non - Hodgkin's lymphoma |
title_fullStr |
p53 gene analysis in childhood B non - Hodgkin's lymphoma |
title_full_unstemmed |
p53 gene analysis in childhood B non - Hodgkin's lymphoma |
title_sort |
p53 gene analysis in childhood B non - Hodgkin's lymphoma |
author |
Klumb,Claudete Esteves Nogueira Pinto |
author_facet |
Klumb,Claudete Esteves Nogueira Pinto Resende,Lídia Maria Magalhães de Tajara,Eloísa Helena Bertelli,Erika Cristina Pavarino Rumjanek,Vivian Mary Maia,Raquel Ciuvalschi |
author_role |
author |
author2 |
Resende,Lídia Maria Magalhães de Tajara,Eloísa Helena Bertelli,Erika Cristina Pavarino Rumjanek,Vivian Mary Maia,Raquel Ciuvalschi |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Klumb,Claudete Esteves Nogueira Pinto Resende,Lídia Maria Magalhães de Tajara,Eloísa Helena Bertelli,Erika Cristina Pavarino Rumjanek,Vivian Mary Maia,Raquel Ciuvalschi |
dc.subject.por.fl_str_mv |
p53 mutation B non-Hodgkin's lymphoma Burkitt's lymphoma |
topic |
p53 mutation B non-Hodgkin's lymphoma Burkitt's lymphoma |
description |
CONTEXT: Mutations or deletions in the tumor-suppressor gene p53 are among the commonest genetic changes found in human neoplasms including breast, lung and bowel cancers. In hematological malignancies, p53 is most often mutated in Burkitt's lymphoma, with p53 mutations present in 30 to 40% of tumor samples and in 70% of cell lines. OBJECTIVE: To analyze the p53 gene alterations in child patients with B non-Hodgkin's lymphoma. DESIGN: Descriptive study. SETTING: Tertiary oncology care center. PARTICIPANTS: The study investigated 12 patients with childhood B non-Hodgkin's lymphoma (Burkitt's lymphoma). Screening for p53 mutations was done by polymerase chain reaction - single strand conformational polymorphism (PCR-SSCP) analysis of exon 5 to 8/9 of the gene. RESULTS: Abnormal polymerase chain reaction - single strand conformational polymorphism migration pattern was observed in 4 patients (33.3%), one on exon 6 and three on exon 7. Positive cases included 2 patients who died from disease. CONCLUSION: These preliminary results suggest that p53 mutations are quite frequent in children with Burkitt's lymphoma and may play a role in lymphoma genesis or disease progression. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802001000600006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802001000600006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-31802001000600006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Paulista de Medicina - APM |
publisher.none.fl_str_mv |
Associação Paulista de Medicina - APM |
dc.source.none.fl_str_mv |
Sao Paulo Medical Journal v.119 n.6 2001 reponame:São Paulo medical journal (Online) instname:Associação Paulista de Medicina instacron:APM |
instname_str |
Associação Paulista de Medicina |
instacron_str |
APM |
institution |
APM |
reponame_str |
São Paulo medical journal (Online) |
collection |
São Paulo medical journal (Online) |
repository.name.fl_str_mv |
São Paulo medical journal (Online) - Associação Paulista de Medicina |
repository.mail.fl_str_mv |
revistas@apm.org.br |
_version_ |
1754209260411027456 |