The application of latent class analysis for diagnostic test validation of chronic Trypanosoma cruzi infection in blood donors
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Infectious Diseases |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000400005 |
Resumo: | The main strategy to prevent transfusion-associated Chagas' disease is the identification of T. Cruzi-infected blood donors by serological screening tests, however there is no perfect serological gold standard. We evaluated an enzyme immunoassay (EIA), an indirect hemaglutination (IHA), and an indirect immunofluorescence (IIF) test for detecting T. Cruzi antibodies in Brazilian blood donors. The results were submitted to latent class analysis, and a radioimmunopreciptation (RIPA) test was performed on repeatedly positive samples. Among 1951 donors, 11 (0.56%) were positive by EIA, 6 (0.31%) by IHA and 16 (0.82%) by IIF. Six samples were positive with all tests, while 4 reacted with EIA and IIF. The RIPA was positive in 6 (75.0%), 7 (66.6%), and 4 (54.0%) samples reacting by the EIA, IHA and IIF tests, respectively. The latent class model detected a high sensitivity rate (100%) for the EIA and IIF, and a specificity rate of 99.95% and 99.69% for the EIA and IIF tests, respectively. The probability of being case according to the model was 99.92% when both EIA and IIF were positive, and 100% for the association of EIA, IIF, and IHA. |
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Brazilian Journal of Infectious Diseases |
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The application of latent class analysis for diagnostic test validation of chronic Trypanosoma cruzi infection in blood donorsChaga's diseaseblood donorsdiagnostic testlatent class analysisThe main strategy to prevent transfusion-associated Chagas' disease is the identification of T. Cruzi-infected blood donors by serological screening tests, however there is no perfect serological gold standard. We evaluated an enzyme immunoassay (EIA), an indirect hemaglutination (IHA), and an indirect immunofluorescence (IIF) test for detecting T. Cruzi antibodies in Brazilian blood donors. The results were submitted to latent class analysis, and a radioimmunopreciptation (RIPA) test was performed on repeatedly positive samples. Among 1951 donors, 11 (0.56%) were positive by EIA, 6 (0.31%) by IHA and 16 (0.82%) by IIF. Six samples were positive with all tests, while 4 reacted with EIA and IIF. The RIPA was positive in 6 (75.0%), 7 (66.6%), and 4 (54.0%) samples reacting by the EIA, IHA and IIF tests, respectively. The latent class model detected a high sensitivity rate (100%) for the EIA and IIF, and a specificity rate of 99.95% and 99.69% for the EIA and IIF tests, respectively. The probability of being case according to the model was 99.92% when both EIA and IIF were positive, and 100% for the association of EIA, IIF, and IHA.Brazilian Society of Infectious Diseases2002-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000400005Brazilian Journal of Infectious Diseases v.6 n.4 2002reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1590/S1413-86702002000400005info:eu-repo/semantics/openAccessLanghi Junior,Dante M.Bordin,José O.Castelo,AdautoWalter,Stephen D.Moraes-Souza,HélioStumpf,Robert J.eng2003-04-01T00:00:00Zoai:scielo:S1413-86702002000400005Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2003-04-01T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false |
dc.title.none.fl_str_mv |
The application of latent class analysis for diagnostic test validation of chronic Trypanosoma cruzi infection in blood donors |
title |
The application of latent class analysis for diagnostic test validation of chronic Trypanosoma cruzi infection in blood donors |
spellingShingle |
The application of latent class analysis for diagnostic test validation of chronic Trypanosoma cruzi infection in blood donors Langhi Junior,Dante M. Chaga's disease blood donors diagnostic test latent class analysis |
title_short |
The application of latent class analysis for diagnostic test validation of chronic Trypanosoma cruzi infection in blood donors |
title_full |
The application of latent class analysis for diagnostic test validation of chronic Trypanosoma cruzi infection in blood donors |
title_fullStr |
The application of latent class analysis for diagnostic test validation of chronic Trypanosoma cruzi infection in blood donors |
title_full_unstemmed |
The application of latent class analysis for diagnostic test validation of chronic Trypanosoma cruzi infection in blood donors |
title_sort |
The application of latent class analysis for diagnostic test validation of chronic Trypanosoma cruzi infection in blood donors |
author |
Langhi Junior,Dante M. |
author_facet |
Langhi Junior,Dante M. Bordin,José O. Castelo,Adauto Walter,Stephen D. Moraes-Souza,Hélio Stumpf,Robert J. |
author_role |
author |
author2 |
Bordin,José O. Castelo,Adauto Walter,Stephen D. Moraes-Souza,Hélio Stumpf,Robert J. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Langhi Junior,Dante M. Bordin,José O. Castelo,Adauto Walter,Stephen D. Moraes-Souza,Hélio Stumpf,Robert J. |
dc.subject.por.fl_str_mv |
Chaga's disease blood donors diagnostic test latent class analysis |
topic |
Chaga's disease blood donors diagnostic test latent class analysis |
description |
The main strategy to prevent transfusion-associated Chagas' disease is the identification of T. Cruzi-infected blood donors by serological screening tests, however there is no perfect serological gold standard. We evaluated an enzyme immunoassay (EIA), an indirect hemaglutination (IHA), and an indirect immunofluorescence (IIF) test for detecting T. Cruzi antibodies in Brazilian blood donors. The results were submitted to latent class analysis, and a radioimmunopreciptation (RIPA) test was performed on repeatedly positive samples. Among 1951 donors, 11 (0.56%) were positive by EIA, 6 (0.31%) by IHA and 16 (0.82%) by IIF. Six samples were positive with all tests, while 4 reacted with EIA and IIF. The RIPA was positive in 6 (75.0%), 7 (66.6%), and 4 (54.0%) samples reacting by the EIA, IHA and IIF tests, respectively. The latent class model detected a high sensitivity rate (100%) for the EIA and IIF, and a specificity rate of 99.95% and 99.69% for the EIA and IIF tests, respectively. The probability of being case according to the model was 99.92% when both EIA and IIF were positive, and 100% for the association of EIA, IIF, and IHA. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000400005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000400005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1413-86702002000400005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
dc.source.none.fl_str_mv |
Brazilian Journal of Infectious Diseases v.6 n.4 2002 reponame:Brazilian Journal of Infectious Diseases instname:Brazilian Society of Infectious Diseases (BSID) instacron:BSID |
instname_str |
Brazilian Society of Infectious Diseases (BSID) |
instacron_str |
BSID |
institution |
BSID |
reponame_str |
Brazilian Journal of Infectious Diseases |
collection |
Brazilian Journal of Infectious Diseases |
repository.name.fl_str_mv |
Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID) |
repository.mail.fl_str_mv |
bjid@bjid.org.br||lgoldani@ufrgs.br |
_version_ |
1754209238289219584 |