Molecular epidemiology of measles virus infection in Shanghai in 2000–2012: the first appearance of genotype D8
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Infectious Diseases |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000600581 |
Resumo: | Purpose:The purpose of this study was to identify measles virus in Shanghai in 2012 and study the genotype trend of measles virus epidemic strains during 2000–2012.Methods:Nose and throat swab specimens were collected from 34 suspected measles cases in Shanghai. Measles virus was isolated using Vero-SLAM cells (African green monkey kidney cells/lymphoid signal activating factor-transfected African green monkey kidney cells). The 450 bp of C terminus of the N gene and the entire hemagglutinin gene sequence was amplified using RT-PCR. Phylogenetic analysis was performed by comparing the seven measles strains in Shanghai with the reference strains for H1a, H1b and D8 genotypes, as well as the Chinese measles virus vaccine strain.Results:Seven measles viruses strains were isolated from the 34 throat swap specimens. Six strains were genotype H1a, which is the predominant strain in China and one strain was genotype D8, which is the first imported strain since 2000. All these seven strains maintained most of the glycosylation sites except subtype H1a, which lost one glycosylation site.Conclusion:Since 2000, measles virus strains in Shanghai are consistent with measles virus from other provinces in China with H1a being the predominant genotype. This study is also the first report of genotype D8 strain in Shanghai. All strains maintained their glycosylation sites except H1a that lost one glycosylation site. These strains could still be neutralized by the Chinese measles vaccine. We suggest that Shanghai Center for Disease Control laboratories should strengthen their approaches to monitor measles cases to prevent further spread of imported strains. |
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Molecular epidemiology of measles virus infection in Shanghai in 2000–2012: the first appearance of genotype D8D8 genotypeImportationMeasles virusMolecular epidemiologyaPurpose:The purpose of this study was to identify measles virus in Shanghai in 2012 and study the genotype trend of measles virus epidemic strains during 2000–2012.Methods:Nose and throat swab specimens were collected from 34 suspected measles cases in Shanghai. Measles virus was isolated using Vero-SLAM cells (African green monkey kidney cells/lymphoid signal activating factor-transfected African green monkey kidney cells). The 450 bp of C terminus of the N gene and the entire hemagglutinin gene sequence was amplified using RT-PCR. Phylogenetic analysis was performed by comparing the seven measles strains in Shanghai with the reference strains for H1a, H1b and D8 genotypes, as well as the Chinese measles virus vaccine strain.Results:Seven measles viruses strains were isolated from the 34 throat swap specimens. Six strains were genotype H1a, which is the predominant strain in China and one strain was genotype D8, which is the first imported strain since 2000. All these seven strains maintained most of the glycosylation sites except subtype H1a, which lost one glycosylation site.Conclusion:Since 2000, measles virus strains in Shanghai are consistent with measles virus from other provinces in China with H1a being the predominant genotype. This study is also the first report of genotype D8 strain in Shanghai. All strains maintained their glycosylation sites except H1a that lost one glycosylation site. These strains could still be neutralized by the Chinese measles vaccine. We suggest that Shanghai Center for Disease Control laboratories should strengthen their approaches to monitor measles cases to prevent further spread of imported strains.Brazilian Society of Infectious Diseases2014-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000600581Brazilian Journal of Infectious Diseases v.18 n.6 2014reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1016/j.bjid.2014.05.018info:eu-repo/semantics/openAccessLi,ShuhuaQian,XiaohuaYuan,ZhenganSun,XiaodongLi,ChongshanTang,XianYang,YanjiGong,XiangzhenCao,Guangweneng2015-09-01T00:00:00Zoai:scielo:S1413-86702014000600581Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2015-09-01T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false |
dc.title.none.fl_str_mv |
Molecular epidemiology of measles virus infection in Shanghai in 2000–2012: the first appearance of genotype D8 |
title |
Molecular epidemiology of measles virus infection in Shanghai in 2000–2012: the first appearance of genotype D8 |
spellingShingle |
Molecular epidemiology of measles virus infection in Shanghai in 2000–2012: the first appearance of genotype D8 Li,Shuhua D8 genotype Importation Measles virus Molecular epidemiologya |
title_short |
Molecular epidemiology of measles virus infection in Shanghai in 2000–2012: the first appearance of genotype D8 |
title_full |
Molecular epidemiology of measles virus infection in Shanghai in 2000–2012: the first appearance of genotype D8 |
title_fullStr |
Molecular epidemiology of measles virus infection in Shanghai in 2000–2012: the first appearance of genotype D8 |
title_full_unstemmed |
Molecular epidemiology of measles virus infection in Shanghai in 2000–2012: the first appearance of genotype D8 |
title_sort |
Molecular epidemiology of measles virus infection in Shanghai in 2000–2012: the first appearance of genotype D8 |
author |
Li,Shuhua |
author_facet |
Li,Shuhua Qian,Xiaohua Yuan,Zhengan Sun,Xiaodong Li,Chongshan Tang,Xian Yang,Yanji Gong,Xiangzhen Cao,Guangwen |
author_role |
author |
author2 |
Qian,Xiaohua Yuan,Zhengan Sun,Xiaodong Li,Chongshan Tang,Xian Yang,Yanji Gong,Xiangzhen Cao,Guangwen |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Li,Shuhua Qian,Xiaohua Yuan,Zhengan Sun,Xiaodong Li,Chongshan Tang,Xian Yang,Yanji Gong,Xiangzhen Cao,Guangwen |
dc.subject.por.fl_str_mv |
D8 genotype Importation Measles virus Molecular epidemiologya |
topic |
D8 genotype Importation Measles virus Molecular epidemiologya |
description |
Purpose:The purpose of this study was to identify measles virus in Shanghai in 2012 and study the genotype trend of measles virus epidemic strains during 2000–2012.Methods:Nose and throat swab specimens were collected from 34 suspected measles cases in Shanghai. Measles virus was isolated using Vero-SLAM cells (African green monkey kidney cells/lymphoid signal activating factor-transfected African green monkey kidney cells). The 450 bp of C terminus of the N gene and the entire hemagglutinin gene sequence was amplified using RT-PCR. Phylogenetic analysis was performed by comparing the seven measles strains in Shanghai with the reference strains for H1a, H1b and D8 genotypes, as well as the Chinese measles virus vaccine strain.Results:Seven measles viruses strains were isolated from the 34 throat swap specimens. Six strains were genotype H1a, which is the predominant strain in China and one strain was genotype D8, which is the first imported strain since 2000. All these seven strains maintained most of the glycosylation sites except subtype H1a, which lost one glycosylation site.Conclusion:Since 2000, measles virus strains in Shanghai are consistent with measles virus from other provinces in China with H1a being the predominant genotype. This study is also the first report of genotype D8 strain in Shanghai. All strains maintained their glycosylation sites except H1a that lost one glycosylation site. These strains could still be neutralized by the Chinese measles vaccine. We suggest that Shanghai Center for Disease Control laboratories should strengthen their approaches to monitor measles cases to prevent further spread of imported strains. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000600581 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000600581 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjid.2014.05.018 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
dc.source.none.fl_str_mv |
Brazilian Journal of Infectious Diseases v.18 n.6 2014 reponame:Brazilian Journal of Infectious Diseases instname:Brazilian Society of Infectious Diseases (BSID) instacron:BSID |
instname_str |
Brazilian Society of Infectious Diseases (BSID) |
instacron_str |
BSID |
institution |
BSID |
reponame_str |
Brazilian Journal of Infectious Diseases |
collection |
Brazilian Journal of Infectious Diseases |
repository.name.fl_str_mv |
Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID) |
repository.mail.fl_str_mv |
bjid@bjid.org.br||lgoldani@ufrgs.br |
_version_ |
1754209243214381056 |